Мультиплексная микрочиповая система с иммобилизованными реактивами для молекулярно-генетического анализа
Moller E.M. A simple and efficient protocol for isolation of highmolecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. / G. Bahnweg, H. Sandermann, H.H. Geiger//Nucleic Acids Research.-1992.-V.20. P. 6115−6116. Chen B.-L. Strategies to suppress aggregation of recombinant keratinocyte growth factor during liquid formulation development /T. Arakawa, E. Hsu, L… Читать ещё >
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1. Разработана схема анализа методом мультиплексной ПЦР-РВ с использованием микрочипов с иммобилизованными реактивами. Разработана аналитическая система, обеспечивающая детектирование флуоресценции двух красителей, в которой для термоциклирования используется элемент Пельтье с управлением по ПИД-алгоритму и корректировкой температуры по АЮС-модели. Разработанная система легла в основу серийного ПЦР-анализатора.
2. В качестве материалов для изготовления микрочипов выбраны кремний и алюминий, обладающие высокой теплопроводностью. Из этих материалов разработаны микрочипы с микрореакторами открытого типа, позволяющие проводить одновременно 16—48 мультиплексных ПЦР-РВ. Стоимость разработанного микрочипа по сравнению с микрофлюидным чипом снижена в 12 раз в случае применения кремния, и в 80 раз — в случае алюминия.
3. Разработаны способы модификации поверхности кремния и алюминия, заключающиеся в создании двумерного распределения гидрофильных и гидрофобных зон путем последовательной обработки поверхности 81 пластины [3-(2,3-эпоксипропокси)-пропил]-триметоксисиланом и этиленгликоль-диметиловым эфиром, и путем обработки А1 пластины в смеси Н3РО4 и ЬШОз и в растворе ПВС для создания гидрофильного покрытия, с нанесением слоя ПММС для создания гидрофобного покрытия. Такая обработка обеспечила высокую эффективность ПНР (94±6% - для 81, 94±5% - для А1), позволила предотвратить ингибирование ПЦР, исключить взаимное смешение образцов.
4. Разработан способ иммобилизации ПЦР-реактивов путем высушивания растворов в микрореакторах и оптимизирован состав стабилизирующих добавок (ЮОтМ трегалоза, 1% Туееп20, 2% ПВП), что позволило достигнуть стабильности при хранении микрочипов при комнатной температуре в течение 4 мес.
5. Показано, что для достижения высокого быстродействия и селективности ПЦР-РВ анализа оптимальная длина амплифицируемого участка ДНК должна находиться в диапазоне от 80 до 150 п.о.
6. Разработаны условия качественного и количественного анализа суспензий микроорганизмов в диапазоне концентраций 102-Ч07 КОЕ/мл методом мультиплексной ПЦР-РВ. Достигнута близкая к максимальной эффективность ПЦР (94+100%). Разработана методика скрининга и количественного определения ГМО с использованием микрочипов с иммобилизованными реактивами. Время анализа составило 23 мин, а предел обнаружения — 0,05% от ГМ растения.
Мультиплексная микрочиповая система с иммобилизованными реактивами для молекулярно-генетического анализа (реферат, курсовая, диплом, контрольная)
1. Meitzer J.S. PCR in Bioanalysis //Humana Press.-1998. pp. 274.
2. Lo Y. M. Clinical applications of PCR. //Humana Press.-1998. pp. 353.
3. Heller, K.J. Genetically Engineered Food. Methods and Detection. //Wiley-VCH.-2003. pp. 304.
4. Mauer J. PCR Methods in food. //Springer Science.-2006. pp. 1485. van Pelt-Verkuil E. Principles and Technical Aspects of PCR Amplification. / A. van Belkum, J.P. Hays //Springer Science.-2008. pp. 332.
5. Abu Al-Soud «W. Capacity of nine thermostable DNA polymerases to mediate DNA amplification in the presence of PCR-inhibiting sample / P. Radstrom // Applied and Environment Microbiology.-1998.-V.64. P. 3748−3753.
6. Chien A. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus / D.B. Edgar, J.M. Trela // Journal of Bacteriology.-1976.-V. 127. P. 1550−1557.
7. Cline J. PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases / J. C Braman, H.H. Hogrefe // Nucleic Acids Research.-1996.-V.24. P. 34 563 451.
8. Belgrader P. Rapid pathogen detection using a microchip PCR array instrument / W. Benett, D. Hadley, G. Long, Jr. R. Mariella, F. Milanovich // Clinical Chemistry.-1998.-V.44.-P. 2191−2194.
9. Rychlik W. Optimization of the annealing temperature for DNA amplification in vitro / W. J. Spencer, R. E. Rhoads // Nucleic Acid Research.-1990.-V.l 8. P. 6409−6412.
10. Altshuler M.L. PCR troubleshooting: the essential guide //Horizon Scientific Press.-2006. pp. 80.
11. Robertson M.J. An introduction to PCR primer design and optimization of amplification reactions / J. Walsh-Weiler // Methods in Molecular Biology.-1998.-V.98. P. 121−154.
12. Kubista M. The real-time polymerase chain reaction // Molecular Aspects of.
13. Medicine.-2006.-V.27.-P. 95−125.
14. Mackay J. Real-Time PCR Fluorescent Chemistries / O. Landt // Methods in Molecular Biology.-2007.-V.353, — P. 237−261.
15. Jothikumar P. Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control / V. Hill, J. Narayanan // BioTechniques.-2009.-V.46. P. 519— 524.
16. Ahmad A.I. New FRET primers for quantitative real-time PCR / J.B. Ghasemi // Analytical and Bioanalytical Chemistry.-2007.-V.3 87. P. 2737−2743.
17. Logan J. Real-time PCR: current technology and applications / K. Edwards, N. Saunders //Horizon Scientific Press.-2009. pp. 284.
18. Duarte С. O. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus / H. de Lencastre //Antimicrobial Agents and Chemotherapy.-2002.-V.46. P. 2155−2161.
19. Rutledge R. G. Mathematics of quantitative kinetic PCR and the application of standard curves / C. Cote //Nucleic Acids Research.-2003.-V.31. P. e93.
20. Ребриков Д. В. ПЦР в реальном времени / Г. А. Саматов, Д. Трофимов //Бином.-2009. pp. 224.
21. Svenstrup H.F. Development of a Quantitative Real-Time PCR Assay for Detection of Mycoplasma genitalium / J.S. Jensen, E. Bjornelius, P. Lidbrink, S. Birkelund, G. Christiansen // Journal of Clinical Microbiology.-2005.-V.43. P. 3121−3128.
22. James D. Reliable detection and identification of genetically modified maize, soybean and canola by multiplex PCR analysis / A.M. Schmidt, E. Wall, M. Green, S. Masri //Journal of agricultural and food chemistry.-2003.-V.51. P. 5829−5834.
23. Mafra I. Food authentication by PCR-based methods / I. Ferreira, B. Oliveira // European Food Research and Technology.-2008.-V.227. P. 649−665.
24. Tianfu H. Validation of internal control for gene expression study in soybean by quantitative real-time PCR / B. Jian, B. Liu, Y. Bi, W. Hou, C. Wu // BMC Molecular Biology.-2008.-V.59. P.
25. Hoorfar J. Practical considerations in design of internal amplification controls for diagnostic PCR assays / B. Malorny, A. Abdulmawjood, N. Cook, M. Wagner, P. Fach // Journal of Clinical Microbiology.-2004.-V.42. P. 1863−1868.
26. Jarosova J. Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR / J.K. Kundu // BMC Plant Biology. -2010.-V.10.-P. 146.
27. Meng S. A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control / J. Li // Virology Journal.-2010.-V.7. P. 117.
28. Shen Y. Identification of suitable reference genes for measurement of gene expression in human cervical tissues / Y. Li, F. Ye, F. Wang, W. Lu, X. Xie // Analytical Biochemistry.-2010.-V.405. P. 224−229.
29. Vandesompele J. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes / K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, Anne De Paepe, F. Speleman // Genome Biology.-2002.-V.18, — P. 3.
30. Wu L.C. Primer design for multiplex PCR using a genetic algorithm / J.T. Horng // Soft Computing.-2007.-V.ll.- P. 855−863.
31. Henegariu O. Multiplex PCR: Critical Parameters and Step-by-Step Protocol / N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt//BioTechniques.-1997.-V.23. P. 504−511.
32. Yuryev A. PCR Primer Design //Humana Press.-2001pp. 431.
33. Everett J.K. Primer Prim’er: A web based server for automated primer design / T.B. Acton, G.T. Montelione // ournal of Structural and Functional Genomics.-2004.-V.5. P. 13−21.
34. Grody W. W. Molecular Diagnostics: Techniques and Applications for the Clinical Laboratory / R.M. Nakamura, F.L. Kiechle //Academic Press.-2009. pp. 484.
35. O’Connell J. RT-PCR Protolols //Humana Press.-2003. pp. 400.
36. Zhang C. PCR microfluidic devices for DNA amplification / J. Xu, W. Ma, W. Zheng // Biotechnology Advances.-2006.-V.24. P. 243−28 441. lightCycler. Carousel-Based System / http://www.roche-applied-science.com.
37. Mathies R.A. Lab-on-a-Chip: miniaturized systems for (bio)chemical analysis and synthesis //Elsevier.-2003. pp. 394.
38. Herold K. Lab on a Chip Technology: Biomolecular Separation and Analysis / A. Rasooly //Horizon Scientific Press.-2009. pp. 300.
39. Fukuba T. Microfabricated flow-through device for DNA amplification / T. Yamamoto, T. Naganuma, T. Fujii // Chemical Engineering Journal.-2004.-V.101. P. 151 156.
40. Park N. Cylindrical Compact Thermal-Cycling Device for Continuous-Flow polymerase chain reaction / S. Kim, J.H. Hahn // Analytical Chemistry.-2003.-V.75. P. 6029−6033.
41. Schneega I. Flow-through polymerase chain reactions in chip thermocyclers / J.M.151.
42. Kohler // Reviews in Molecular Biotechnology.-2001.-V.82. P. 101−121.
43. Giordano B.C. Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds / J. Ferrance, S. Swedberg, F.R. Huhmer, J.P. Landers // Analytical Biochemistry.-2001 .-V.291. P. 124−132.
44. Neuzil P. Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes / C. Zhang, J. Pipper, S. Oh, L. Zhuo //Nucleic Acids Research.-2006.-V.34. P. e77.
45. Lodhi M.A. A simple and efficient method for DNA extraction from grapevine cultivars and vitis species. / G.N. Ye, N.F. Weeden, B.I. Reisch // Plant Molecular Biology Reporter.-1994.-V. 12.-P. 6−13.
46. Tian W.C. Microfluidics for Biological Applications / E. Finehout //Springer.-2008.-pp. 416.
47. Bilitewski U. Microchip Methods in Diagnostics //Humana press.-2009. pp. 183.
48. Shoffner M.A. Chip PCR II. Investigation of different PCR amplification systems in microbabricated silicon-glass chips / J. Cheng, J.E. Hvichia, L.J. Kricka, P. Wilding // Nucleic Acids Research.-1996.-V.24. P. 380−385.
49. Liu R.J. Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection / J. Yang, R. Lenigk, J. Bonanno, P. Grodzinski //Analytical Chemistry.-2004.-V.76. P. 1824−1831.
50. Northrup M.A. A miniature analytical instrument for nucleic acids based on micromachined silicon reaction chambers // Analytical Chemistry.-1998.-V.70. P. 918−922.
51. Chen J.H. A novel micro-well array chip for liquid phase biomaterial processing and detection / T.F. Chen, S.R. Huang, J. Gong, J.C. Li, W.C. Chen, T.H. Hseu, I.C. Hsu // Sensors and Actuators.-2003.-V.108. P. 193−200.
52. Matsubara Y. Application of a microchamber array for DNA amplification using a152novel dispensing method / M. Kobayasni, Y. Morita, E. Tamiya // Archives of Histology and Cytology.-2002.-V.65. P. 481−488.
53. Nagai H. Development of a microchamber array for picoliter PCR / Y. Murakami, Y. Morita, K. Yokoyama, E. Tamiya //Analytical Chemistry.-2001.-V.73. P. 1043−1047.
54. Nagai H. High-throughput PCR in silicon based microchamber array / Y. Murakami, K. Yokoyama, E. Tamiya // Biosensors & Bioelectronics.-2001.-V.16. P. 1015- 1019.
55. Akagi Y. Optimization of fluorescent cell-based assays for high-throughput analysis / S. Ramachandra, Y. Morita, E. Tamiya // Science and Technology of Advanced Materials.-2004.-V.5.-P. 343−349.
56. Tamiya E. On-chip nanoliter-volume multiplex TaqMan polymerase chain reaction from a single copy based on counting fluorescence released // Anal. Chem.-2004.-V.76. P. 6434−6439.
57. Brenan C. High throughput, nanoliterquantitative PCR / T. Morrison // Drug Discovery Today: Technologies.-2005.-V.2. P. 247−253.
58. Liu J. Solving the «World-to-Chip» interface problem witha microfluidic matrix / C. Hansen, S.R. Quake //Analytical Chemistry.-2003.-V.75. P. 4718−4723.
59. Seeb J.E. SNP genotyping by the 5'-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms / C.E. Pascal, R. Ramakrishnan, L.W. Seeb // Methods in Molecular Biology.-2009.-V.583. P. 277−292.
60. Сляднев M.H. Разработка мультиреакторной микрофлюидной системы для ПЦР анализа в режиме реального времени / А. А. Танеев, В. А. Казаков, М. В. Лаврова, М. А. Еркин // Журнал аналитической химии.-2008.Л/.63. Р. 210−217.
61. Shoffner М.А. Chip PCR I. Surface passivation of microfabricated silicon-glasschips for PCR / J. Cheng, J.E. Hvichia, L.J. Kricka, P. Wilding // Nucleic Acids Research.1 531 996.-V.24.-P. 375−379.
62. Erill I. Bacteriophage surface display of an immunoglobulin-binding domain of Staphylococcus aureus protein A / S. Campoy, N. Erill, J. Barbe, J. Aguilo // Sensors and Actuators.-2003.-V.96.-P. 685−692.
63. Fukuba T. Microfabricated flow-through device for DNA amplification / T. Yamamoto, T. Naganuma, T. Fujii // Chemical Engineering Journal.-2004.-V.101. P. 151— 156.
64. Krishnan M. Polymerase chain reaction in high surface-to-volume ratio Si02 microstructures / D.T. Burke, M.A. Burns // Analytical Chemistry.-2004.-V.76. P. 65 886 593.
65. Koh C.J. Integrating polymerase chain reaction, valving, and electrophoresis in a plastic device for bacterial detection / W. Tan, M. Zhao, A.J. Ricco, Z.H. Fan // Analytical Chemistry.-2003.-V.75. P. 4591−4598.
66. Grodzinski P. High sensitivity PCR assay in plastic micro reactors // Lab Chip.-2002.-V.2. P. 179−187.
67. Felbel J. Chemical surface management for micro PCR in silicon chip thermocyclers /1. Bieber, J.M. Kohler // Proceedings of SPIE.-2002.-V.49. P. 34−40.
68. Schmidt U. Low-volume amplification on chemically structured chips using the PowerPlexl6 DNA amplification kit // International Journal of Legal Medicine.-2006.-V.l20.-P. 42—48.
69. Brenan J.H. Nanoliter high throughput quantitative PCR // Nucleic Acids Research.-2006.-V.34. P. el23.
70. Kim J. A disposable, self-contained PCR chip / D. Byun, M.J. Mauka, H.H. Bau // Lab Chip.-2009.-V.9. P. 606−612.
71. Drobyshev A.L. The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format / TV. Nasedkina, N.V. Zakharova // Biomicrofluidics.-2009.-V.3. P. 44 112.
72. Mikhailovich V.M. An oligonucleotide microarray for multiplex real-time PCR identification of HIV-1, HBV and HCV / D.A. Khodakov, N.V. Zakharova, D.A. Gryadunov, F.P. Filatov, A.S. Zasedatelev // BioTechniques.-2008.-V.44, — P. 241−248.
73. Rey L. Freeze-Drying/Lyophilization of Pharmaceutical and Biological Products / J.C. May //Marcel Dekker.-1999. pp. 501.
74. Klatser P.R. Stabilized, freeze-dried PCR mix for detection of mycobacteria. / S. Kuijper, C.W. van Ingen, J.H. Kolk // Journal of Clinical Microbiology.-1998.-V.36. P. 1798−1800.
75. Carpenter J.F. Liophilization of protein pharmaceuticals / B.S. Chang // Biotechnology and biopharmaceutical manufacturing.-1996.-V.- P. 199−264.
76. Aracawa T. Factors affecting short-term and long-term stabilities of protein / J. Prestrelsky, W. Kinney, J.F. Carpenter //Advanced Drug Delivery.-1993.-V. 10. P. 1−28.
77. Prestrelsky J. Dehydration-induced conformational transitions in proteins and their inhibition by stabilizers / N. Tedeschi, T. Aracawa, J.F. Carpenter // Biophysics.-1993.-V.65.-P. 661−671.
78. Levine H. Principles of «criostabilization» technology from structure/propertiey relations / L. Slade // Cryo Letters.-1988.-V.9. P. 21−63.
79. Chilson O.P. Effect of freezing on enzymes / L.A. Costello, N.O. Kaplan // Fed Proc.-.-V.24. P. 55−65.
80. Chen B.-L. Strategies to suppress aggregation of recombinant keratinocyte growth factor during liquid formulation development /T. Arakawa, E. Hsu, L. Narhu, TJ. Tressel, S.L. Chen // Journal of Pharmaceutical Sciences.-.-V.83.~ P. 1657−1661.
81. Carpenter J.F. Stabilization of phosphofructorokinase with sugars during freeze-drying / L.M. Crowe, J.H. Crowe // Biochem Biophys Acta.-1987.-V.923. P. 109−115.
82. Izutsu K. Decreased protein-stabilizing effects of cryoprotectants due to crystallization / S. Yoshioka, T. Teora // Pharmaceutical Research.-.-V.1993. P. 1232−1237.
83. Carpenter J.F. An infrared spectroscopic study of the interactions of carbohydrates with dried proteins / J.H. Crowe // Biochemistry.-1989.-V.28. P. 3916−3922.
84. Izutsu K. Effect of mannitol crystallinity on the stabilization of enzymes / S. Yoshioka, T. Teora // Chemical & Pharmaceutical Bulletin.-1994.-V.42. P. 5−8.
85. Izutsu K. The effects of additives on the stability of freeze-dried (3-galactosidase. stored at elevated temperature / S. Yoshioka, Y. Takeda // International Journal of Pharmaceutics.-1991.-V.71. P. 137−146.
86. Chang B.S. Surface-induced denaturation of proteins during freezing and its inhibition by surfactants / B.S. Kendrick, J.F. Carpenter // Pharmaceutical Research.-1996.-V.85.-P. 1325−1333.
87. Патент. Dry amplification reagent mixure for PCR and the technique of PCR analysis // G.O. Shaikhaev.-2005. W02005/103 217.
88. DIANE Publishing Company. Biotechnology in a Global Economy //DIANE Publishing.-1992.-pp. 283.
89. Nelson G.C. Genetically modified organisms in agriculture: economics and politics //Academic Press.-2001. pp. 344.
90. Marmiroli N. Methods for detection of GMOs in food and feed / E. Maestri, M, Gulli, A, Malcevschi, C, Peano, R, Bordoni, G, De Bellis // Analytical and Bioanalytical Chemistry.-2008.-V.392. P. 369−384.
91. О надзоре за оборотом пищевых продуктов, содержащих ГМО / Федеральная служба по надзору в сфере защиты прав потребителей и благополучия человека. Постановление № 80 от 30.11.2007.
92. Определение генетически модифицированных микроорганизмов и микроорганизмов, имеющих генетически модифицированные аналоги, в пищевых продуктах методами полимеразной цепной реакции (ПЦР) в реальном времени /. МУК 4.2.2305−07.
93. Kempken F. Genetic Modification of Plants: Agriculture, Horticulture and Forestry / C. Jung //Springer Berlin Heidelberg.-2010. pp. 667.
94. Grohmann L. Detection of genetically modified plantsin seeds, food and feed // Biotechnology in Agriculture and Forestry.-2010.-V.64. P. 117−136.
95. Vollenhofer S. Genetically modified organisms in foods screening and specific detection by polymerase chain reaction / K. Burg, J. Schmidt, H. Kroath // Journal of agricultural and food chemistiy.-1999.-V.47. P. 5038−5043.
96. Kricka L.J. Microchip PCR / P. Wilding // Anal Bioanal Chem.-2003.-V.377. P. 820−825.
97. Reyes D.R. Micro Total Analysis Systems. 1. Introduction, Theory and Technology / D. Iossifidis, P. Auroux, A. Manz // Analytical Chemistry.-2002.-V.74. P. 2623−2636.
98. Oda R.P. Infrared-mediated thermocycling for ultrafast poly-merase chain reaction / M.A. Strausbauch, A.F.R. Huhmer, N. Borson, S.R. Jurrens, J. Craig //Analytical Chemistiy.-1998.-VP. 4361 4368.
99. Huhmer A.F.R. Noncontact infrared-mediated thermo-" cycling for effective polymerase / J.P. Landers //Analytical Chemistry.-2000.-V.72. P. 5507 5512.
100. Orrling K. An efficient method to perform milliliter-scale PCR utilizing highly controlled microwave thermocycling / P. Nilsson, M. Gullberg, M. Larhed // Chemical Communications.-2004.-V.7. P. 790−791.
101. Fermer C. Microwave-assisted high-speed PCR / P. Nilsson, M. Larhed //157.
102. European Journal of Pharmaceutical Sciences.-2003.-V.18. P. 129−32.
103. Arumuganathan K. Nuclear DNA content of some important plant species / E.D. Earle // Plant Molecular Biology Reporter.-1991.-V.9. P. 208−218.
104. Shenhar R. Self-assembly and self-organization / T. Norsten, V. Rotello // Nanostructure Science and Technology.-2004.-V.2. P. 41−74.
105. ИЗ. Патент. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety // H. Yang, S. Sundberg.-2005.-US6841193.
106. Кнунянц И.JI. Химическая энциклопедия //Советская энциклопедия.-1988.-рр.
107. Richards J.У. Aluminium: its history, occurrence, properties, metallurgy and applications //BiblioBazaar.-2009. pp. 346.
108. Патент. Reaction system for performing in the amplification of nucleic acids // M. Lee, H. Bird, D. Leslie, D. Squirrel, J. Shaw, D. Ween, D. Diacon -2007.-W0/2001/23 093.
109. Takahashi H. Anodic oxide films on aluminum: their significance for corrosion protectio / S. Masatoshi, T. Kikuchi // Modern Aspects of Electrochemistry.-2009.-V.46. P. 59−174.
110. Vargel C. Corrosion of aluminium //Elsevier.-2004. pp. 626.
111. Davis J.R. Corrosion of aluminum and aluminum alloys //ASM International.-1999. pp. 313.
112. Roper M.G. Advances in polymerase chain reaction on microfluidic chips / C.J. Easley, J.P. Landers //Analytical Chemystry.-2005.-V.77. P. 3887−3894.
113. Золотов Ю. А. Основы аналитической химии //Высшая школа.-2000. pp. 351.
114. Булатов М. И. Практическое руководство по фотометрическим методам анализа / И. П. Калинкин //Химия.-1986. pp. 432.
115. Ljung L. Modeling of dynamic systems / T. Glad //PTR Prentice Hall.-1994. pp. 361.
116. Chaudhari A.M. Transientliquid crystal thermometry of microfabricated PCRvessel arrays / T.M. Woudenberg, M. Albin, K.E. Goodson // Microelectromechanical158systems.-1998.-V.7. P. 345 55.
117. Curcio M. Continuous segmented-flow polymerase chainreaction for high-throughput miniaturized DNA amplification / J. Roeraade // Analytical Chemystry.-2003.-V.75. P. 1−7.
118. Wang Q. An integrated software solution for real-time PCR analysis based on microfluidic biochip / Gong H. // Bioengineered and Bioinspired Systems.-2003.-.-77 — 86.
119. Wang Q. An integrated system for real-time PCR analysis based on microfluidic biochip / Y. Tan, H. Gong // International Journal of Computational Engineering Science.-2003.-V.4. P. 285−288.
120. Кельнер P. Аналитическая химия. Проблемы и подходы. //Мир.-2004. pp. 608.
121. Lee Т. A miniaturized DNA amplifier: its application in traditional Chinese medicine / I.-M.Hsing, A. Lao, M.C. Carles //Analytical Chemistry.-2000.-V.72. P. 42 424 247.
122. Mackay I.M. Real-time PCR in virology / E.A. Katherine, A. Nitsche // Nucleic Acids Research.-2002.-V.30. P. 1292−1305.
123. Spadoro J.P. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance // Journal of Clinical Microbiology.-1998.-V.36. P. 191 197.
124. Siegmund V. Dry-reagent-based PCR as a novel tool for laboratory confirmation // Journal of Clinical Microbiology.-2005.-V.43. P. 271−276.
125. Skrabanja A.T. Lyophilization of biotechnology products / A.L. de Meere, R.A. de Ruiter, P.J. van den Oetelaar // Journal of Pharmaceutical Science and Technology.-1994.-V.48.-P. 311−317.
126. Marmiroli N. Methods for detection of GMOs in food and feed / E. Maestri, M. Gulli, A. Malcevschi, C. Peano, R. Bordoni, G. De Bellis // Analytical and Bioanalytical Chemistry.-2008.-V.392. P. 369−384.
127. Yeates C. Methods for microbial DNA extraction from soil for PCR amplification /.
128. M.R.Gillings, A.D.Davison, N. Altavilla, D.A.Veal // Biological Procedures Online.-1998.1591. V.l.-P.
129. Moller E.M. A simple and efficient protocol for isolation of highmolecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. / G. Bahnweg, H. Sandermann, H.H. Geiger//Nucleic Acids Research.-1992.-V.20. P. 6115−6116.
130. Lodhi M. A simple and efficient method for DNA extraction from grapevine sultivars and vitis species / G.-N. Ye, N. Weeden, B. Reisch // Plant Molecular Biology Reporter.-1994.-V. 12.-P. 6−13.
131. Pitcher D.G. Rapid extraction of bacterial genomic DNA with guanidiumthiocyanate / N.A. Saunders, R.J. Owen // Letters in Applied Microbiology.-1989.-V.8.-P. 151−156.
132. Berensmeier S. Magnetic particles for the separation and purificationof nucleic acids //Applied Microbiology and Biotechnology.-2006.-V.73. P. 495−504.
133. Choudary P.V. Adsorption of endogenous polyphenols relieves the inhibition byfruit juices and fresh produce of immuno-PCR detection of Escherichia coli 0157: H7 / A.A. Ogunjimi // FEMS Immunology and Medical Microbiology.-1999.-V.23. P. 213−220.
134. Koonjul P. Inclusion of polyvinylpyrrolidone in the polymerasechain reaction reverses the inhibitory effects ofpolyphenolic contamination of RNA / W. Brandt, J. Farrant, G. Lindsey // Nucleic Acids Research.-1999.-V.27. P. 915−916.
135. Tulpan D. Thermodynamically based DNA strand design / M. Andronescu, S.B. Chang, M.R. Shortreed, A. Condon, H. H. Hoos, L.M. Smith // Nucleic Acids Research.2005.-V.33.-P. 4951−4964.
136. Ho S. Thermogenomics: Thermodynamic-based approaches to genomicanalyses of DNA structure // Methods.-2009.-V.47. P. 159−167.
137. Tanaka E Specificity of Hybridization Between DNA Sequences Based on Free Energy. / A. Kameda, M. Yamamoto, A. Ohuchi // Lecture Notes in Computer Science.2006.-V.3892.-P. 371−379.
138. SantaLucia J.Jr. The thermodynamics of DNA structural motifs. / D. Hicks // Annu Rev Biophys Biomol Struct.-2004.-V.33. P. 415−440.
139. Hardegger M. Quantitative detection of the 35S promoter and the NOS terminatorusing quantitative competitive PCR / P. Brodmann, A. Herrmann // European Food Research and Technology.-1999.-V.209. P. 83−87.
140. Ishii K. Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR / M. Fukui // Applied and Environmental Microbiology. -2001.-V.67.-P. 3753−3755.
141. Moreira B.G. Effects of fluorescent dyes, quenchers, and dangling endson DNA duplex stability. / Y. You, M.A. Behlke, R. Owczarzy // Biochemical and Biophysical Research Communications.-2005.-V.327. P. 47384.