Изучение механосенситивных фибробластов желудочков сердца здоровых крыс и крыс после экспериментального инфаркта миокарда
Диссертация
Мембранный потенциал и МЗР фибробластов ткани сердца чувствительны к гипоксии и последующей реоксигенацией. В условиях гипоксии и в начальный период реоксигенации, когда active force препарата уменьшалась и resting force увеличивалась, мембранный потенциал уменьшался до -5 ± 1 mV. Эти изменения наблюдались одновременно с уменьшением амплитуды МЗР вплоть до полного его исчезновения. В этих… Читать ещё >
Содержание
- ПЕРЕЧЕНЬ УСЛОВНЫХ ОБОЗНАЧЕНИЙ И ТЕРМИНОВ
- ГЛАВА I. ОБЗОР ЛИТЕРАТУРЫ
- 1. Характеристики сердечных фибробластов
- 2. Общие представления о механосенситивности и механосенситивном канале
- 3. Роль механосенситивных каналов в механизмах генерации механоиндуцированного потенциала
- 4. Функциональная классификация МСК
- 5. Роль внутриклеточного Са2+ в генерации механоиндуцированного потенциала
- 6. Роль механосенситивных каналов в формировании электрического ответа клетки
- 7. Роль цитоскелета в регуляции воротного механизма МСК и в механизмах генерации механоиндуцированного потенциала
- 8. Зависимость механоиндуцированных потенциалов фибробластами сердца от возраста животных
- 9. Характеристика ишемии миокарда
- 10. Фибробласты предсердий животных, перенесших инфаркт миокарда, как субстрат механоэлектрической обратной связи
- ГЛАВА II. МАТЕРИАЛЫ И МЕТОДЫ ИССЛЕДОВАНИЙ
- 1. Объект исследований
- 2. Метод получения инфаркта левого желудочка
- 3. Метод электрокардиографии
- 4. Метод получения препарата правого предсердия
- 5. Метод получения препарата из околорубцовой зоны левого желудочка крыс, перенесших инфаркт миокарда
- 6. Получение изолированных фибробластов
- 7. Перфузионная среда для микроэлектродных исследований ткани
- 8. Растворы для исследований изолированных фибробластов методом patch-clamp
- 9. Микроэлектроды
- 10. Patch-пипетки
- 11. Экспериментальная установка для микроэлектродных исследований клеток в ткани
- 11. 1. Электронно-измерительная система
- 11. 2. Система жизнеобеспечения изолированного препарата сердца
- 11. 3. Электронно-измерительная аппаратура для регистрации сократительной активности фрагментов сердца и их механической стимуляции
- 12. Факторы воздействия на клетки при микроэлектодных исследованиях
- 12. 1. Электрические стимулы
- 12. 2. Механические раздражители
- 12. 3. Создание гипоксии
- 13. Экспериментальная установка для исследований ионных токов фибробластов методом patch-clamp в конфигурации whole-cell
- 13. 1. Электронно-измерительная система
- 13. 2. Система жизнеобеспечения изолированных фибробластов
- 14. Воздействия на клетки при исследованиях методом patch-clamp
- 14. 1. Электрические стимулы
- 14. 2. Химические соединения
- 14. 3. Механические раздражители
- 15. Характеристика регистрируемых параметров при микроэлектродых исследованиях
- 15. 1. Характеристика регистрируемых параметров фибробластов
- 15. 2. Характеристика регистрируемых параметров кардиомиоцитов
- 16. Характеристика регистрируемых параметров при исследованиях методом patch-clamp
- 17. Методы обработки результатов экспериментов
- ГЛАВА III. РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЕ
- ЧАСТЬ 1. ИССЛЕДОВАНИЕ ИОННЫХ ТОКОВ ИЗОЛИРОВАННЫХ ФИБРОБЛАСТОВ ПРИ ИХ СЖАТИИ И РАСТЯЖЕНИИ
- 1. Электрофизиологическая идентификация недеформированных сердечных фибробластов
- 2. Сжатие и растяжение фибробластов
- 2. 1. Сжатие фибробластов увеличивает мембранную проводимость
- 2. 2. Растяжение уменьшает проводимость мембраны фибробластов
- 2. 3. Исследование вольт-амперных характеристик на фоне сжатия и растяжения клетки
- 3. Обсуждение
- ЧАСТЬ 2. ИССЛЕДОВАНИЕ ВЛИЯНИЯ ГИПОКСИИ/РЕОКСИГИНАЦИИ НА
- ЭЛЕКТРОФИЗИОЛОГИЧЕСКИЕ СВОЙСТВА ФИБРОБЛАСТОВ В ТКАНИ СЕРДЦА
- 1. Электрофизиологические свойства фибробластов при высоком напряжении кислорода (80 кРа)
- 2. Электрический ответ сердечных фибробластов на гипоксиюреоксигенацию и его корреляция с resting force и active force
- 3. Влияние растяжения на электрофизиологические параметры сердечных фибробластов в условиях высокого напряжения кислорода
- 4. Изменения потенциала покоя фибробластов предсердия и частоты спонтанных сокращений препарата в период гипоксиии/реоксигенации
- 5. Обсуждение
- ЧАСТЬ 3. ИЗУЧЕНИЕ РОЛЬ ФИБРОБЛАСТОВ ЖЕЛУДОЧКА В МЕХАНО-ЭЛЕКТРИЧЕСКОЙ ОБРАТНОЙ СВЯЗИ НА ФОНЕ ПОСТИНФАРКТНОГО РЕМОДЕЛИНГА
- 1. Характеристика сердца в процессе постинфарктного ремоделинга
- 1. 1. Идентификация зоны инфаркта
- 1. 2. Характеристики кардиомиоцитов из зоны прилегающей к рубцу
- 1. 3. Основные электрофизиологические характеристики фибробластов в процессе ремоделинга
- 1. 4. Обсуждение
- 1. 4. 1. Электрофизиологические свойства сердечных фибробластов после инфаркта миокарда
- 1. 4. 2. Возможная роль фибробластов желудочков после инфаркта миокарда как субстрата механоэлектрической обратной связи
- 1. Характеристика сердца в процессе постинфарктного ремоделинга
- 2. Фибробласты левого желудочка как субстрат механоэлектрической обратной связи в сердце после инфаркта миокарда левого желудочка
- 2. 1. Характеристики механоэлектрической обратной связи у фибробластов
- 2. 2. Обсуждение
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