Исследование метаболизма инсулина в эритроцитах крыс в норме и при экспериментальном диабете
Диссертация
Для организации диабетологической службы создан Федеральный диабетологический центр, а также будут организованы диабетологические центры (отделения) субъектов Российской Федерации на базе имеющихся эндокринологических диспансеров или вновь организуемых отделений больниц с обязательным выделением профильных служб по лечению осложнений сахарного диабета (ретинопатии, нефропатии, ИБС, диабетической… Читать ещё >
Содержание
- ГЛАВА I. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Роль инсулина в обмене веществ. Молекулярные механизмы действия инсулина на клетки
- 1. 2. 1. Структура рецепторов инсулина
- 1. 2. 2, Аутофосфорилирование рецепторов инсулина
- 1. 3. Роль специфического связывания инсулина плазматическими мембранами клеток в его расщеплении
- 1. 4. Методы исследования инсулин деградирующей системы клеток
- 1. 5. 1. Ферменты, расщепляющие инсулин
- 1. 5. 2. Инсулиназа и ее свойства.'
- 1. 5. 3. Роль инсулиназы в клетке
- 1. 5. 4. Мембранная инсулиназа. 40 1.6.1. Ингибиторы инсулиназы. 41 1.7. Продукты деградации инсулина инсулиназой
- 1. 8. 1. Деградация инсулина в эритроцитах
- 1. 8. 2. Связывание инсулина рецепторами эритроцитов крыс в норме и при патологии. 48 1.8.3 Связывание инсулина рецепторами эритроцитов человека в норме и при инсулин зависимом диабете
- 1. 8. 4. Инсулин деградирующая активность эритроцитов при диабете
- 1. 9. Патогенез развития сахарного диабета
- 1. 10. Методы лабораторной диагностики сахарного диабета
- 1. 11. Вклад в изучение метаболизма инсулина кафедры
- 1. 1. Роль инсулина в обмене веществ. Молекулярные механизмы действия инсулина на клетки
- Ленинградского химико-фармацевтического института
- ГЛАВА II. МАТЕРИАЛЫ И МЕТОДЫ. 2.1. Объекты исследования
- 2. 2. Определение количества эритроцитов в эритроцитарной массе
- 2. 3. Создание экспериментального диабета
- 2. 4. Выделение и очистка инсулиназы из лизата эритроцитов
- 2. 4. 1. Получение лизата эритроцитов
- 2. 4. 2. Ионообменная хроматография на DEAE сефадексе
- 2. 4. 3. Аффинная хроматография
- 2. 5. Выделение инсулиназы из цитозоля гепатоцитов
- 2. 6. Получение препарата инсулина, меченного I
- 2. 7. Методы определения активности инсулиназы
- 2. 8. Изучение влияния ионов металлов на активность инсулиназы
- 2. 9. Определение оптимума действия инсулиназы
- 2. 10. Получение термо-кислото устойчивого ингибитора из лизата эритроцитов
- 2. 11. Выделение ингибитора инсулиназы из гепатоцитов
- 2. 12. Изучение обмена инсулиназы из цитозоля гепатоцитов
- 2. 13. Определение концентрации белка
- 2. 13. 1. Определение наличия гликозидной части в составе ингибитора инсулиназы
- 2. 13. 2. Определение наличия липидной части в составе ингибитора инсулиназы
- 2. 14. Определение связывания инсулина рецепторами мембраны эритроцитов
- 2. 14. 1. Определение процента специфического связывания инсулина рецепторами мембраны эритроцитов
- 2. 14. 2. Определение количества мест связывания инсулина на эритроцит
- 2. 15. Электрофоретические методы анализа фермента
- 2. 15. 1. Электрофорез в пластинах полиакриламидного геля
- 2. 15. 2. Диск — электрофорез в градиентном ПААГ
- 2. 15. 3. Электрофорез с добавлением ДС натрия
- 2. 15. 4. Окраска полиакриламидных гелей для выявления белковых полос
- 2. 16. Статистическая обработка результатов
- ГЛАВА III. РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЯ
- 3. 1. Определение процента специфического связывания инсулина рецепторами мембраны эритроцитов
- 3. 2. Изучение сродства инсулиновых рецепторов мембраны эритроцита крысы в норме и при экспериментальном диабете
- 3. 3. Определение количества инсулиновых рецепторов на мембране эритроцита крысы в норме и при экспериментальном диабете
- 3. 4. 1. Выделение и очистка инсулиназы из эритроцитов
- 3. 5. Характеристика инсулиназы из лизата эритроцитов
- 3. 5. 1. Определение оптимума рН для инсулиназы лизата эритроцитов
- 3. 5. 2. Изучение влияния ингибиторов и активаторов на активность инсулиназы из лизата эритроцитов
- 3. 5. 3. Определение кинетических параметров инсулиназы из лизата эритроцитов
- 3. 5. 4. Изучение инсулин деградирующей активности эритроцитов в норме и при экспериментальном диабете
- 3. 6. Изучение активности эндогенного ингибитора инсулиназы из лизата эритроцита
- 3. 6. 1. Изучение ингибитора инсулиназы из плазмы крови
- 3. 7. Изучение мембранной инсулиназы эритроцитов
- 3. 8. Изучение деградации инсулина в гепатоцитах
- 3. 9. Характеристика инсулиназы из цитозоля гепатоцитов
- 3. 9. 1. Изучение субстратной специфичности инсулиназы из гепатоцитов
- 3. 9. 2. Изучение кинетических параметров инсулиназы цитозоля гепатоцитов
- 3. 10. Характеристика термо-кислото устойчивого ингибитора из цитозоля гепатоцитов
- 3. 10. 1. Активность высокомолекулярного ингибитора при экспериментальном диабете
- 3. 11. Обмен инсулиназы из цитозоля гепатоцитов
- 3. 12. Исследование инсулин рецепторного и инсулин деградирующего комплекса эритроцитов человека
- 3. 12. 1. Определение процента специфического связывания инсулина рецепторами мембраны эритроцитов человека
- 3. 12. 2. Изучение сродства инсулиновых рецепторов мембраны эритроцита человека в норме и при инсулин зависимом диабете
- 3. 12. 3. Определение количества инсулиновых рецепторов на мембране эритроцита человека в норме и при инсулин зависимом диабете
- 3. 12. 4. Выделение и очистка инсулиназы из эритроцитов человека
- 3. 13. Характеристика инсулиназы из лизата эритроцитов человека
- 3. 13. 1. Определение оптимума рН для инсулиназы лизата эритроцитов человека
- 3. 13. 2. Изучение влияния ингибиторов и активаторов на активность инсулиназы из лизата эритроцитов человека
- 3. 13. 3. Определение кинетических параметров инсулиназы из лизата эритроцитов человека
- 3. 13. 4. Изучение инсулин деградирующей активности эритроцитов человека в норме и при инсулин зависимом диабете
- 3. 14. 1. Изучение активности эндогенного ингибитора инсулиназы из лизата эритроцита человека
- 3. 14. 2. Изучение ингибитора инсулиназы из плазмы крови человека
- IV. ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ
- ВЫВОДЫ
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