Иммуноцитохимический анализ ядерных антигенов лимфоцитов человека, выявляемых с помощью моноклональных антител
На основании клинико-лабораторного анализа обследуемых лиц (30 здоровых и 70 больных с различными лимфопролиферативными заболеваниями) показана положительная корреляция между экспрессией антигенов В23 и Ki-67 (г=0,2- р<0,05). Выявлены статистически значимые различия (р<0,05) по степени экспрессии В23 в лимфоцитах здоровых лиц и больных всеми нозологическими формами лимфопролиферативных… Читать ещё >
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- Список используемых сокращений
ГЛАВА I. ОБЗОР ЛИТЕРАТУРЫ «Ядерные антигены, ассоциированные с пролиферацией клеток, и методы их выявления».
1.1. Понятие «клеточный цикл», его фазы, состояние 12 пролиферативного покоя («вне цикла»), принципы регуляции клеточного цикла.
1.2. Ядерные антигены пролиферирующих клеток (Ki-67, PCNA, 15 ДНК-полимераза альфа и т. д.).
1.3. Основной ядрышковый белок В23.
ГЛАВА II. МАТЕРИАЛЫ И МЕТОДЫ ИССЛЕДОВАНИЯ.
ГЛАВА III. РЕЗУЛЬТАТЫ СОБСТВЕННЫХ ИССЛЕДОВАНИЙ.
3.1. Подбор оптимальных иммуноцитохимических способов 60 выявления ядрышкового антигена с помощью моноклонального антитела ЗС9.
3.2. Идентификация антигена, выявляемого антителом ЗС9.
3.3. Изучение локализации антигена, выявляемого МКА ЗС9, в 70 динамике клеточного цикла.
3.4. Изменения в локализации В23 при стимуляции клеток к 72 пролиферации.
3.5. Исследование лимфоцитов доноров и больных с различными 82 формами лимфопролиферативных заболеваний с помощью непрямого иммунопероксидазного метода.
3.6. Получение новых гибридом, продуцирующих моноклональные 90 антитела к ядерным антигенам лейкоцитов крови человека.
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