Антирестрикционный белок ArdA, кодируемый трансмиссивной плазмидой IncI группы: мутационный анализ и изучение механизма действия
Диссертация
Показано, что белок ArdA значительно более эффективно ингибирует рестрикционную, чем модификационную активность системы рестрикции-модификации 1-го типа клетки-хозяина. Предполагается, что это свойство белка ArdA может иметь важное значение для успешной адаптации немодифицированной ДНК трансмиссивной плазмиды при вхождении в новую бактериальную клетку в процессе конъюгации. При помощи топкого… Читать ещё >
Содержание
- Список сокращений
- I. Введение
- II. Литературный обзор
- ПЛ. Трансмиссивные плазмиды
- II. 2. Системы рестрикции-модификации ДНК у бактерий
- 11. 2. 1. Три типа систем рестрикции-модификации
- 11. 2. 2. Системы рестрикции-модификации 1-го типа
- 11. 2. 2. 1. Субъединица узнавания
- 11. 2. 2. 2. Субъединица модификации
- 11. 2. 2. 3. Модифицирующий фермент
- 11. 2. 2. 4. Субъединица рестрикции
- 11. 2. 2. 5. Комплекс рестрикции-модификации 1-го типа
- 11. 2. 3. Системы бактерий, гидролизующие специфически модифицированную ДНК
- II. 2. Системы рестрикции-модификации ДНК у бактерий
- III. 1. Штаммы E. coli, использованные в работе
- 111. 2. Плазмиды и бактериофаги, использованные в работе
- 111. 3. Среды для выращивания бактерий и фагов
- 111. 3. 1. Жидкие среды
- 111. 3. 2. Твердые среды
- 111. 5. Выращивание культур бактерий
- 111. 6. Выращивание бактериофагов
- 111. 7. Высев бактерий и бактериофагов. Опыты по изучению эффективности рестрикции
- 111. 7. 1. Высев бактерий
- 111. 7. 2. Высев бактериофагов
- 111. 7. 3. Опыты по изучению эффективности рестрикции
- 111. 8. Методы работы с плазмидной ДНК
- 111. 8. 3. Электрофорез ДНК в агарозном геле
- 111. 8. 4. Электроэлюция фрагментов ДНК из агарозного геля
- 111. 8. 5. Рестрикционный аиализ плазмид
- 111. 8. 6. Протокол клонирования фрагментов ДНК в вектор
- 111. 9. Получение однотяжевых ДНК-матриц
- 111. 11. Сайт-направленный мутагенез
- II. 12. Определение нуклеотидной последовательности ДНК
- III. 13. Получение делеций с помощью Bal 31 нуклеазы
- III. 15. Мечение фрагмента ДНК при помощи реакции ПЦР
- 111. 16. Идентификация белков в системе экспрессии на основе Т7 РНК полимеразы
- 111. 19. Электрофорез белков в денатурирующих условиях
- 111. 20. Замедление подвижности ДНК в геле
- 111. 21. Измерение промоторной активности с использованием системы вектора рКК
- 111. 22. Очистка белков при помощи аффинной хроматографии на Ni-NTA колонке
- 111. 23. Связывание белков in vitro
- 111. 24. Определение агрегатного состояния белка ArdA, с использованием метода гель-фильтрации
- IV. 1. Картирование области белка ArdA, существенной для его функционирования с помощью тонкого делеционного анализа
- IV. 2. Идентификация аминокислотных остатков белка ArdA, которые существенны для его функционирования
- IV. 3. Изучение взаимодействия [35Б]-меченных белка ArdA и
- IV. 4. Определение агрегатного состояния белка ArdA в растворе
- IV. 5. Белок ArdA блокирует способность R-M системы
- IV. 7. Белок ArdA более эффективно ингибирует рестрикциониую, чем модификационную активность ЕсоК1-системы in vivo
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