Разработка метода иммобилизации белков в микрочипах для иммуноферментного анализа
Диссертация
Завершение геномного проекта ставит следующую грандиозную задачу в биологических исследованиях — понимание функции открытых генов. Один из популярных подходов, названный «Протеомикой» по аналогии с «Геномикой», состоит в использовании комбинации двумерного электрофореза в полиакриламидном геле с последующим масс-спектрометрическим анализом первичной структуры разделенных денатурированных белков… Читать ещё >
Содержание
- СПИСОК ИСПОЛЬЗУЕМЫХ СОКРАЩЕНИЙ
- ГЛАВА 1. ВВЕДЕНИЕ
- ГЛАВА 2. ОБЗОР ЛИТЕРАТУРЫ
- I. Методы изготовления микрочипов
- 1. Синтез вещества на микроматрице
- 2. Методы нанесения вещества на микрочипы
- 2. 1. Последовательные методы
- 2. 2. Параллельные методы
- II. Электронапыление. Различные применения и подробное описание метода
- 1. Применение электронапыления для изготовления тонких источников радиоактивности
- 2. Применение электрораспыления в масс-спектрометрии
- 2. 1. Явление электрораспыления. Теория метода
- 3. Сохраняют ли молекулы нативное состояние при электрораспылении?
- 4. Применение электрораспыления для изучения третичной структуры белковых ионов в газовой фазе
- 5. Применение электронапыления в изготовлении образцов для туннельной микроскопии
- 6. Другие известные применения явления электрораспыления
- 7. Применение электронапыления для изготовления микрочипов
- III. Методы иммобилизации белков на микрочипах
- 1. Адсорбция
- 2. Химическое привязывание
- 2. 1. Пришивание глутаровым альдегидом
- 2. 2. Ковалентная пришивка через производные силана
- 2. 3. Пришивка белков к поверхности золота через тиоловые группы
- 2. 4. Декстрановые спейсоры для пришивки белков и способы их активации
- 2. 5. Пришивка белков через Ж^-концевые гистидины (Н1з
- 3. Иммобилизация в пористых средах
- 3. 1. Иммобилизация белков на фильтрах
- 3. 2. Иммобилизация белков в ПААГ
- IV. Анализ методов регистрации в иммунохимическом гетерогенном анализе
- 1. РИА
- 2. ФИА
- 3. ИФА
- V. Конкретные примеры изготовления и применения белковых микрочипов
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- Пичугин A.B., Персональное сообщение1. БЛАГОДАРНОСТИ.
- Благодарю моего мужа за любовь, терпение и помощь в компьютерной обработке результатов и верстке диссертационной работы. Благодарю моего сына за терпение и поддержку.
- Благодарю моих родителей Морозова В. Н. и Морозову Т. Я. за неоценимый опыт, активную помощь в работе и обсуждении результатов и вообще за все.
- Благодарю Атаулаханова Ф. И. мудрое руководство, помощь, доброту и чуткость.
- Благодарю Пичугина А. В. за помощь в освоении метода ИФА и активное участие в обсужднии результатов.
- Благодарю моих рецензентов Бутылина А. А. и Сударикова А. Б. за внимательное прочтение диссертации и ценные замечания и советы. Благодарю Реука В. Д. за ценные советы и помощь в подготовке диссертационной работы к защите.
- Благодарю всех сотрудников лаборатории физической биохимии системы крови и всего Гематологического научного центра за помощь и участие. Мне было очень приятно работать в месте с ними.