Взаимодействие поверхностных рецепторов клетки с различными иммобилизованными и свободными лигандами определяет структуру актинового цитоскелета
Диссертация
Состояние цитоскелета клеток, находящихся в составе ткани многоклеточного организма, зависит от сложной комбинации биологически активных молекул, взаимодействующих с поверхностными рецепторами. В культивируемых клетках цитоскелет формируется в процессе распластывания на субстрате и может зависеть от природы субстрата, от состава питательной среды, плотности культуры и факторов сыворотки крови… Читать ещё >
Содержание
- 1. ВВЕДЕНИЕ
- 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Организация актинового цитоскелета в культивируемых клетках
- 2. 1. 1. Основные типы актиновых структур
- 2. 1. 2. Роль актин-связывающих белков в формировании актиновых структур
- 2. 1. 3. Формирование актиновых структур in vivo в процессе распластывания клетки на субстрате
- 2. 2. Реорганизация актинового цитоскелета под действием внешних факторов
- 2. 3. Малые ГТФазы Rho, Rac и Cdc42 и их роль в регуляции структуры актинового цитоскелета
- 2. 4. Комплексы адгезии. Связь актиновых структур с сигнальными молекулами
- 2. 5. Неопластическая трансформация как фактор, вызывающий реорганизацию актинового цитоскелета
- 2. 6. Транскрипционный фактор NF-кВ. Участие в процессах адгезии
- 2. 1. Организация актинового цитоскелета в культивируемых клетках
- ЗАДАЧИ ИССЛЕДОВАНИЯ
- 3. МАТЕРИАЛЫ И МЕТОДЫ
- 3. 1. Клетки и клеточные культуры
- 3. 2. Материалы
- 3. 3. Подготовка субстратов
- 3. 4. Изучение адгезии клеток на различных субстратах
- 3. 5. Изучение влияния различных иммобилизованных лигандов на структуру актинового цитоскелета
- 3. 6. Конъюгация моноклональных антител с флуорохромом
- 3. 7. Иммунофлуоресценция
- 3. 8. Приготовление цитозоля фибробластов и клеток линии А
- 3. 9. Аффинная хроматография на колонках с иммобилизованным F-актином
- 3. 10. Электрофорез и идентификация белков
- 4. РЕЗУЛЬТАТЫ
- 4. 1. Различия в морфологии и организации актинового цитоскелета в клетках А-431, распластанных на разных лигандах
- 4. 1. 1. Адгезия клеток А-431 на субстратах, покрытых разными лигандами
- 4. 1. 2. Специфичность адгезии клеток А-431 на субстрате, покрытом антителами к рецептору ЭФР
- 4. 1. 3. Различия в морфологии и организации актинового цитоскелета в клетках А-431, распластанных на разных лигандах
- 4. 2. Различия в морфологии и организации актинового цитоскелета в нормальных, иммортализованных и трансформированных фибробластах, распластанных на разных лигандах
- 4. 3. Различия в морфологии и организации актинового цитоскелета в целомоцитах полихеты Arenicola marina, распластанных на разных лигандах
- 4. 4. Распределение F-актина в клетках А-431, находящихся на ранних стадиях распластывания на разных субстратах
- 4. 5. Распределение актин-связывающих белков в клетках А-431, распластанных на разных иммобилизованных лигандах
- 4. 5. 1. Структура актинового цитоскелета в клетках А-431, распластанных на иммобилизованном конканавалине А
- 4. 5. 2. Распределение винкулина, талина, а-актинина и спектрина в клетках А-431, распластанных на разных субстратах
- 4. 6. Влияние активаторов малых ГТФаз на структуру актинового цитоскелета
- 4. 6. 1. Влияние культивирования в бессывороточной среде на структуру актинового цитоскелета в клетках А-431 и фибробластах крысы
- 4. 6. 2. Ранний ответ клеток на действие ЭФР, LPA и ВК
- 4. 6. 3. Последовательные стадии реорганизации системы микрофиламентов под действием ЭФР, LPA и ВК в клетках, распластанных на разных субстратах
- 4. 6. 4. Зависимость распределение винкулина от действия LPA и ВК
- 4. 6. 5. Распределение Р-актина в клетках А-431 на разных стадиях распластывания и его перераспределение под действием ЭФР, LP, А и ВК
- 4. 7. Зависимость распределения транскрипционного фактора NF-кВ в цитоплазме нормальных и трансформированных фибробластов крысы от состояния актинового цитоскелета
- 4. 7. 1. Распределение p65/RelA в цитоплазме нормальных фибробластов
- 4. 7. 2. Перераспределение p65/RelA в ElA+cHa-Ras-трансформированных фибробластах при распластывании их на фибронектине
- 4. 7. 3. Разборка актинового цитоскелета под действием цитохалазина D сопровождается перераспределением p65/RelA в цитоплазме
- 4. 7. 4. p65/RelA концентрируется у ведущего края ламеллы в ElA+cHa-Ras-трансформированных фибробластах, распластанных на ламинине 2/
- 4. 7. 5. Распределение p65/RelA в клетках А
- 4. 7. 6. Взаимодействие p65/RelA с фибриллярным актином in vitro
- 4. 1. Различия в морфологии и организации актинового цитоскелета в клетках А-431, распластанных на разных лигандах
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