Бактериальная трансформация и иммобилизация тяжелых металлов и радионуклидов
Диссертация
Негу et al., 2003). Были изолированы 5 штаммов Okibacterium frillariae и 6 штаммов Rhodococcus fascians, и 1 штамм Microbacterium sp., устойчивые к 5−12 мМ Ni, из опада и почвы вокруг Ni-гипераккумулирующего растения Thlaspi goesingense (Idris et al., 2004). Кроме того, два штамма Arthrobacter, устойчивых к никелю, были выделены из почвы вокруг Ni-гипераккумулирующих растений (Schlegel et al… Читать ещё >
Содержание
- ЧАСТЬ 1. ВВЕДЕНИЕ
- Актуальность проблемы
- Цели и задачи работы
- Научная новизна и значимость работы
- Практическая ценность работы
- Апробация работы
- Основные защищаемые положения
- Список публикаций по теме диссертации
- ЧАСТЬ 2. ОБЗОР ЛИТЕРАТУРЫ ГЛАВА
- 2. 1. Ядерные топливные циклы
- 2. 1. 1. Типы ядерных топливных циклов
- 2. 1. 2. Преимущества и недостатки различных вариантов ЯТЦ
- 2. 1. 3. ЯТЦ в разных странах
- 2. 1. 4. Состав типичных отходов
- 2. 1. Ядерные топливные циклы
- 2. 2. Свойства исследованных радионуклидов и тяжелых 34 металлов
- 2. 2. 1. Технеций
- 2. 2. 2. Уран
- 2. 2. 3. Плутоний
- 2. 2. 4. Хром
- 2. 3. Взаимодействие микроорганизмов с долгоживущими 51 радионуклидами
- 2. 3. 1. Взаимодействие с актинидами
- 2. 3. 2. Взаимодействие технеция с микроорганизмами
- 2. 4. Микроорганизмы-редукторы металлов, металлоидов и 57 радионуклидов — современное состояние вопроса
- 3. 1. Материалы и методы
- 3. 2. Сорбция радионуклидов в нейтральных условиях
- 3. 3. Восстановление технеция сульфатредуцирующими бактериями в нейтральных условиях
- 3. 3. 1. Анаэробная ассоциация сульфатредуцирующих бактерий
- 3. 3. 2. Удаление технеция из буферных растворов чистыми 82 культурами сульфатредуцирующих бактерий
- 3. 3. 3. Удаление технеция из раствора иммобилизованной биомассой 85 сульфатредуцирующих бактерий
- 3. 4. Восстановление семивалентного технеция в кислых условиях среды
- 3. 4. 1. Удаление пертехнетата из раствора при росте A. ferrooxidans
- 3. 4. 2. Рост ацидитиобацилл с использованием элементной серы и 90 пертехнетата
- 3. 4. 3. Резистентность тиобацилл к технецию на примере 91 A. ferrooxidans
- 3. 5. Восстановление технеция в щелочных условиях
- 3. 5. 1. Исследование восстановления пертехнетата в щелочных 97 условиях оптическим и хроматографическим методами
- 3. 5. 2. Возможные доноры электронов и токсическое действие технеция 103 на процесс восстановления пертехнетата в щелочных условиях
- 3. 6. Восстановление хромата в щелочных условиях
- 3. 7. Восстановление шестивалентного урана в нейтральных условиях термофильной бактерией Carboxydothermus ferrireducens
- 3. 7. 1. Восстановление шестивалентного урана термофильной 117 бактерией при использовании различных источников углерода
- 3. 7. 2. Рост термофильной бактерии при различных концентрациях 119 урана
- 3. 7. 3. Динамика бактериального роста и восстановления 126 шестивалентного урана
- 3. 7. 4. Анализ осадков, содержащих уран, до и после восстановления 126 термофильной бактерией Thermoterrabacterium ferrireducens
- 3. 8. Влияние микроорганизмов, обитающих в пресных водоемах, 138 на технеций и некоторые трансурановые элементы в случае их попадания в водоем
- 3. 8. 1. Удаление радионуклидов илами пресноводных озер
- 3. 8. 2. Детекция зонального распределения радионуклидов между 144 илами и водной фазой пресноводных озер на примере плутония
- 3. 9. Токсическое воздействие двухвалентных катионов тяжелых 147 металлов и шестивалентных анионов металлов и радионуклидов на микроорганизмы
- 3. 9. 1. Общая характеристика сайта
- 3. 9. 2. Выделение аэробных микроорганизмов из зараженных почв и 151 характеристика их устойчивости к металлам и радионуклидам
- 3. 9. 3. Устойчивость изолятов к никелю и урану
- 3. 10. Возможные механизмы адаптации микроорганизмов к 162 высоким концентрациям тяжелых металлов
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