Исследование экзоцитоза в нейтрофилах человека при миелопролиферативных заболеваниях
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Диссертация
Миелопролиферативные заболевания представляют собой группу опухолей кроветворной ткани, возникающих в результате злокачественной трансформации стволовой кроветворной клетки, и характеризующиеся способностью клеточных элементов к дифференцировке и созреванию (Raskind W.H., Steinmann L., Najfeld V., 1998; Tefferi A., 2001). Ранее в нейтрофилах периферической крови больных миелопролиферативными… Читать ещё >
Содержание
- Глава 1. Обзор литературы 1.1 .Миелопролиферативные заболевания
- 1. 1. 1. Хронический миелолейкоз
- 1. 1. 2. Эритремия
- 1. 1. 3. Сублейкемический миелоз (миелофиброз)
- 1. 2. Гранулы нейтрофилов человека
- 1. 2. 1. Характеристика и классификация гранул нейтрофилов
- 1. 2. 1. 1. Азурофильные (первичные) гранулы
- 1. 2. 1. 2. Специфические (вторичные) гранулы
- 1. 2. 1. 3. Желатиназные гранулы
- 1. 2. 1. 4. Секреторные везикулы
- 1. 2. 2. Экзоцитоз гранул нейтрофилов
- 1. 3. Гипотеза слипания и слияния мембран (SNARE гипотеза)
- 1. 3. 1. Характеристика основных белковых компонентов модели 1.3.1.1 .Белки семейства VAMP/синаптобревинов
- 1. 3. 1. 2. Синтаксины
- 1. 3. 1. 3. SNAP
- 1. 3. 1. 4. NSF
- 1. 3. 1. 5. SNAP
- 1. 3. 2. Механизм слипания/слияния мембран (SNARE гипотеза)
- 1. 3. 1. Характеристика основных белковых компонентов модели 1.3.1.1 .Белки семейства VAMP/синаптобревинов
- 2. 1. Объекты исследования
- 2. 2. Материалы
- 2. 3. Методы
- 2. 3. 1. Выделение нейтрофилов человека
- 2. 3. 2. Активация нейтрофилов
- 2. 3. 3. Определение активности миелопероксидазы
- 2. 3. 4. Определение активности лизоцима
- 2. 3. 5. Определение активности щелочной фосфатазы
- 2. 3. 6. Иммунофлуоресцентная микроскопия
- 2. 3. 7. Электронная микроскопия
- 2. 3. 8. Электрофорез белков
- 2. 3. 9. Иммуноблоттинг (Вестерн-блот анализ) 49 2. 3.9.1. Перенос фракций из геля на фильтр 50 2.3.9.2. Иммунное выявление антигенов на фильтре
- 2. 3. 10. Иммунопреципитация
- 3. 1. Оценка экзоцитоза внутриклеточных гранул нейтрофилов больных миелопролиферативными заболеваниями
- 3. 2. Идентификация белков-медиаторов экзоцитоза в лейкоцитах пациентов с миелопролиферативными заболеваниями
- 3. 3. Взаимодействие белков группы SNARE в нейтрофилах больных миелопролиферативными заболеваниями
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