Изучение функций различных доменов нового транскрипционного коактиватора SAYP (E (y) 3) Drosophila melanogaster и поиск взаимодействующих с ним белков
Диссертация
Считается, что именно первый этап экспрессии генов — транскрипция — является наиболее важным в регуляции всего процесса. У эукариот различные наборы генов транскрибируются специализированными РНК-полимеразамигены, кодирующие мРНК, транскрибируются РНК-полимеразой И. Многочисленные исследования показали, что на активацию транскрипции РНК-полимеразой II влияют несколько дискретных, контролируемых… Читать ещё >
Содержание
- ОБЗОР ЛИТЕРАТУРЫ
- 1. ИНИЦИАЦИЯ ТРАНСКРИПЦИИ РНК-ПОЛИМЕРАЗОЙIIУ ЭУКАРИОТ
- 1. 1. РНК-полимераза П
- 1. 2. Локусы контроля транскрипции генов класса II
- 1. 3. Базальная транскрипция. Общие факторы транскрипции
- 1. 4. Специфические факторы транскрипции: активаторы и репрессоры
- 1. 5. Корегуляторы
- 2. ХРОМАТИН
- 2. 1. Структура хроматина. Модификации гистонов
- 2. 2. Структурные и биохимические особенности гетерохроматина
- 2. 3. Гетерохроматин и транскрипция. Эффект положения гена
- 2. 4. Белковые комплексы, изменяющие структуру хроматина
- 3. ЯДЕРНЫЕ РЕЦЕПТОРЫ
- 3. 1. Строение и механизмы действия ядерных рецепторов
- 3. 2. Ядерные рецепторы и гормональная сигнализация у D. melanogaster
- 3. 3. Корегуляторы ядерных рецепторов
- ОБЪЕКТ И ЗАДАЧИ ИССЛЕДОВАНИЯ
- МАТЕРИАЛЫ И МЕТОДЫ
- 1. МАТЕРИАЛЫ
- 1. 1. Штаммы и вектора S. cerevisiae и Е. coli, использованные в работе
- 1. 2. Библиотека кДНК
- 1. 3. Дрожжевые и бактериальные среды
- 1. 4. Ферменты и реактивы
- 1. 5. Праймеры
- 1. 6. Конструкции для анализа активации репортерных генов в дрожжах
- 1. 7. Конструкции pBait для двухгибридного анализа
- 1. 8. Конструкция для экспрессии фрагмента белка Нг46 для получения поликлональных антител
- 1. 9. Конструкции для анализа функций доменов белка SAYP in vivo
- 2. МЕТОДЫ
- 2. 1. Работа с дрожжами. Скрининг библиотеки кДНК в двухгибридной системе
- 2. 2. Работа с ДНК
- 2. 3. Работа с белками
- 2. 4. Работа с D. melanogaster
- 2. 5. Использованное программное обеспечение
- РЕЗУЛЬТАТЫ
- 1. Изучение роли SAY-домена белка SAYP в активации транскрипции в дрожжах
- 2. Изучение влияния белка SAYP на транскрипцию в гетерохроматине
- 3. Изучение влияния SAY-домена белка SAYP на выживаемости и активацию транскрипции in vivo
- 4. Изучение роли PHD-доменов белка SAYP в репрессии транскрипции в гетерохроматине
- 5. Поиск белков, взаимодействующих с SAYP в двухгибридной системе
- 6. Подтверждение взаимодействия SAYP и Hr46(DHR3)
- ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ
- ВЫВОДЫ
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