Изучение антибактериального действия низкомолекулярного поликатионного пептида варнерина на антибиотикорезистентные штаммы Staphylococcus epidermidis
Диссертация
В последние годы главенствующее место в этиологии инфекционных заболеваний вновь занимает грамположительная микрофлора, в том числе бактерии рода Staphylococcus (Бухарин и др., 2002). Применение антибиотиков как в условиях стационаров, так и при амбулаторном лечении постоянно формирует новые штаммы стафилококков, отличающиеся повышенной устойчивостью к антибактериальным химиопрепаратам. Так… Читать ещё >
Содержание
- Обзор литературы
- Глава 1. УСТОЙЧИВОСТЬ МИКРООРГАНИЗМОВ К АНТИБИОТИКАМ. АНТИБАКТЕРИАЛЬНЫЕ ПЕПТИДЫ И ПЕРСПЕКТИВА ИХ ИСПОЛЬЗОВАНИЯ ДЛЯ БОРЬБЫ С АНТИБИОТИКОРЕЗИСТЕНТНЫМИ ШТАММАМИ МИКРООРГАНИЗМОВ
- 1. 1. Проблема формирования антибиотикоустойчивости у микроорганизмов
- 1. 1. 1. Антибиотикорезистентность и механизмы ее появления у микроорганизмов
- 1. 1. 2. Механизмы устойчивости бактерий к антибактериальным средствам
- 1. 1. 3. Пути преодоления устойчивости бактериальных клеток к антибиотикам
- 1. 2. Антибактериальные пептидные соединения как природные факторы ингибирования роста микроорганизмов
- 1. 2. 1. Пептиды млекопитающих
- 1. 2. 2. Пептиды земноводных
- 1. 2. 3. Пептиды насекомых
- 1. 2. 4. Пептиды растений
- 1. 2. 5. Пептиды бактерий
- 1. 2. 6. Механизмы антибактериального действия поликатионных пептидов
- 1. 2. 7. Практическое использование поликатионных пептидов
- 1. 1. Проблема формирования антибиотикоустойчивости у микроорганизмов
- 2. 1. Бактериальные штаммы
- 2. 2. Среды и условия культивирования
- 2. 3. Определение антибиотикочувствительности исследуемых штаммов
- 2. 4. Получение антибиотикорезистентных штаммов S. epidermidis
- 2. 5. Определение интенсивности роста и количества жизнеспособных клеток
- 2. 6. Получение препарата низкомолекулярного катионного пептидного антибактериального фактора
- 2. 7. Количественное определение антибактериального пептида в использованных препаратах
- 2. 8. Определение антибактериальной активности варнерина
- 2. 9. Выделение плазмидной ДНК
- 2. 10. Анализ плазмидного спектра
- 2. 11. Анализ липидного состава цитоплазматических мембран клеток
- 2. 12. Статистическая обработка данных
- 3. 1. Получение антибиотикорезистентных штаммов S. epidermidis
- 3. 2. Бактерицидное действие антибактериального пептида варнерина на различные штаммы S. epidermidis
- 3. 3. Влияние варнерина на антибиотикочувствительность клеток различных штаммов S. epidermidis
- 3. 4. Влияние различных по механизму действия антибиотиков на чувствительность клеток S. epidermidis к варнерину
- 3. 5. Роль трансмембранного потенциала клеток S. epidermidis в реализации бактерицидного эффекта варнерина
- 3. 6. Действие варнерина на клетки S. epidermidis в комбинации с лизоцимом
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- Отдельное спасибо Александру Юрьевичу Максимову за помощь, оказанную им при оформлении диссертационной работы.
- Выражаю благодарность доктору биологических наук Олегу Николаевичу Октябрьскому за предоставление среды для определения антибиотикочувствительности бактерий.
- Большое спасибо рецензентам кандидату биологических наук Татьяне Никодимовне Каменских и кандидату биологических наук Елене Генриховне Плотниковой за ценные советы и замечания, сделанные ими при прочтении данной работы.
- Считаю своим долгом поблагодарить доктора биологических наук, профессора Ирину Борисовну Ившину за неоценимую помощь и поддержку на заключительном этапе выполнения работы.