Клеточные механизмы иммунного ответа на вакцину BCG у мышей линии CBA/N-xid
![Диссертация: Клеточные механизмы иммунного ответа на вакцину BCG у мышей линии CBA/N-xid](https://westud.ru/work/2529962/cover.png)
Диссертация
Туберкулез (ТБ) до сих пор является одним из самых распространенных инфекционных заболеваний и остается одной из основных причин гибели людей в мире, что делает проблему его изучения очень актуальной. При соблюдении баланса между собственными защитными реакциями и иммунным ответом хозяина Mycobacterium tuberculosis может, не вызывая реальной болезни, сохранять состояние латентности на протяжении… Читать ещё >
Содержание
- Список сокращений
- Глава 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Туберкулез — особенности патогенеза и иммунного ответа
- 1. 2. Противотуберкулезные вакцины (BCG, и другие живые вакцины, субъединнчные вакцины)
- 1. 3. Мутация Xid и ее роль в иммунном ответе и чувствительности к инфекциямЛ
- 1. 4. Влияние мутации xid на функции клеток иммунной системы
- 1. 4. 1. Роль мутации xid в днфференцировке и функционировании В-лимфоцитов
- 1. 4. 2. Макрофаги, их функции и роль Btk
- 1. 4. 3. Влнянне мутации xid на функции нейтрофилов
- 1. 4. 4. Влнянне мутации xid на функции дендритных клеток
- 1. 4. 5. Влияние мутации xid на проведение сигнала от TLRs
- 1. 5. Роль мутации xid в туберкулезной инфекции
- Глава 2. МАТЕРИАЛЫ И МЕТОДЫ
- 2. 1. Лабораторные животные
- 2. 2. Микобактериальные культуры
- 2. 3. Заражением tuberculosis H37Rv
- 2. 4. Вакцинация мышей
- 2. 5. Определение количества микобактерий в органах зараженных животных
- 2. 6. Антигены
- 2. 7. Среды и растворы
- 2. 8. Получение суспензии клеток легкого
- 2. 9. Получение суспензии клеток регионарных лимфатических узлов
- 2. 10. Получение суспензии клеток селезенки
- 2. 11. Получение суснензин перитонеальных макрофагов
- 2. 12. Гистопатология
- 2. 13. Фагоцитоз микобактерий перитонеальными макрофагами
- 2. 14. Окраска клеток, содержащих микобактерии
- 2. 15. Получение Т-линий
- 2. 16. Антигсн-спсцифическин пролиферативный тест
- 2. 17. Получение костномозговых химер
- 2. 18. Перенос клеток эмбриональной нечени
- 2. 19. Анализ клеток методом проточной цитофлуориметрии
- 2. 20. Выделение суммарной РНК нз суспензии клеток
- 2. 21. Получение кДНК
- 2. 22. Определение продукции цитокинов и хемокинов клетками методом ПЦР в реальном времени
- 2. 23. Определение количества Т-лимфоцитов, продуцирующих IFNy, методом IFNy secretion assay in vitro
- 2. 24. Определение количества Т-лимфоцтов, продуцирующих IFNy, методом ELISPOT in vitro
- 2. 25. Удаление нейтрофилов in vivo
- 2. 26. Статистическая обработка результатов
- Глава 3. РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЕ
- 3. 1. 1. Влияние мутации xid на клеточный состав органов до и после вакцинации
- 3. 1. 2. Влияние мутации xid на клеточный состав органов зараженных животных
- 3. 2. Оценка числа Т-лимфоцитов CD4+, продуцирующих IFNy в легких вакцинированных мышей после заражения
- 3. 3. Зависимость эффективности вакцинации мышей CBA/N-xid от дозы вводимой BCG
- 3. 4. Роль В-лимфоцитов в формировании иммунного ответа на туберкулезную инфекцию
- 3. 4. 1. Влияние переноса Т и В лимфоцитов мышей СВА мышам CBA/N на восстановление их способности к вакцинации
- 3. 4. 2. Перенос В-клеток также восстанавливает способность к вакцинации мышей CBA/N
- 3. 4. 3. Оценка способности мышей линии CBA/N формировать протективный иммунный ответ, при вакцинации соинкатом микобактерий
- 3. 5. Влияние мутации xid на функции макрофагов
- 3. 6. Оценка влияния мутации xid на способность макрофагов презентировать антиген Т-лимфоцитам
- 3. 7. Особенности миграции нейтрофилов мышей линий СВА и CBA/N-xid
- 3. 8. Оценка экспрессии генов хемокинов, участвующих в миграции нейтрофилов
- 3. 9. Эффект блокирования нейтрофилов у мышей CBA/N при вакцинации BCG на последующее заражение туберкулезом
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