Механизмы формирования внутреннего центромерного домена кинетохора позвоночных животных
Диссертация
Сборка функционального кинетохора происходит заново в ходе каждого клеточного цикла. Считается, что сборка наружного кинетохора происходит в строгом иерархическом порядкет. е. связывание одних компонентов регулируется присутствием на кинетохоре других. (Johnson et al., 2004; Sharp-Baker and Chen, 2001; Vigneron et al., 2004; Wong and Fang, 2006). Несмотря на значительные успехи в изучении… Читать ещё >
Содержание
- ГЛАВА 1.
- ВВЕДЕНИЕ
- 1. 1. Актуальность проблемы
- 1. 2. Цели и задачи исследования
- ГЛАВА 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Цитоплазматические экстракты ооцитов шпорцевой лягушки Хепориз 1аеу1 $ как модельная система изучения событий клеточного цикла
- 2. 2. Строение и функция кинетохора позвоночных
- 2. 2. 1. Центромер
- 2. 2. 2. Наружный кинетохор
- 2. 2. 2. 1. Строение наружного кинетохора
- 2. 2. 2. 2. Регуляция сборки НК
- 2. 2. 2. 3. Функции наружного кинетохора 24 В настоящеем разделе мы рассмотрим механизмы, отвечающие за каждую из этих функций
- 2. 2. 3. Строение и функция внутреннего центромерного домена 33 2.2.3.1 Транзитный хромосомный комплекс
- 2. 2. 3. 1. 2.1. Роль ТХК в регуляции митотической конденсации и поддержании структуры хромосом
- 2. 2. 3. 1. 2.2 Роль ТХК в регуляции сегрегации хромосом
- 2. 2. 3. 1. 2.3 Роль ТХК в 8АС
- 2. 2. 3. 1. 2.4 Роль ТХК в формировании веретена деления
- 2. 2. 3. 1. 2.5 Роль ТХК в цитокинезе 45 2.2.3.3 Кинезин МСАК 46 2.2.3.3 Комплекс 51п^051ип/РР2А
- 2. 2. 3. Регуляция сборки ВЦЦ
- 3. 1. Рекомбипантые белки и антитела
- 3. 2. Приготовление цитоплазматического экстракта из ооцитов X. 1аеУ!
- 3. 3. Очистка хроматина 59 3.4 Клеточные линии, их культивирование, получение стабильных клеточных линий, РНК-интерференция
- 3. 5. Иммунофлуоресценция и анализ изображений
- 3. 6. Иммунопреципитация и гг-преципитация
- 3. 7. Вестерн-блотинг
- 3. 8. Киназная активность in vitro
- 4. 1. Выбор модели для изучения строения и регуляции формирования кинетохора
- 4. 2. Регуляция расположения транзитного хромосомного комплекса во внутреннем центромерном домене кинетохора
- 4. 2. 1. Белок Bubi контролирует расположение транзитного хромосомного комплекса во внутреннем центромерном домене в системе экстрактов ооцитов Xenopus iaevis
- 4. 2. 2. Нарушение расположения ТХК на кинетохоре в отсутствие Bubi не связано с задержкой в прохождении митоза
- 4. 2. 3. Bubi контролирует расположение ТХК во внутреннем центромерном домене в соматических клетках
- 4. 2. 4. Киназная активность Bubi абсолютно необходима для связывания ТХК с внутренним центромерным доменом
- 4. 2. 5. Bubi контролирует стабильность транзитного хромосомного комплекса
- 4. 2. 6. Bubi фосфорилирует INCENP 83 4.3. Регуляция расположения белка Shugoshin в ВЦД
- 4. 3. 1. Динамика xenopus Sgo в ходе клеточного цикла
- 4. 3. 2. Bubi контролирует связывание Sgo с митотическим хроматином
- 4. 3. 3. Для связывания xSgo с митотическим хроматином не требуется накопления киназы Bubi на кинетохоре 89 4.3.4 Транзитный хромосомный комплекс необходим для правильного расположения Sgo на кинетохоре
- 4. 4. Регуляция расположения кинезина МСАК в ВЦД
- 4. 5. Формирование внутреннего центромерного домена и наружного кинетохора происходит независимо от митотической конденсации хроматина
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