Клонирование и исследование нового гена Camello в раннем развитии Xenopus laevis
Диссертация
Различные представители семейства кадгеринов были найдены у Xenopus. По времени экспрессии их разделяют на два подкласса — материнские, начинающие экспрессию до включения зародышевого генома, то есть до МВТ, и зиготические — после МВТ. Зиготическими являются Е-кадгерин, экспрессирующийся в эктодерме и N-кадгерин, появляющийся во время нейральной индукции. Недавно открытый РАРС (Paraxial… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ ОБЗОР ЛИТЕРАТУРЫ Раннее развитие Xenopus laevis
- Гены, участвующие в эмбриональной индукции
- Регуляция клеточных движений на стадии гаструлы
- Семейство ацетилтрансфераз
- 1. Функции ацетилтрансфераз в цитоплазме
- 2. Ядерные ацетилтрансферазы
- 3. Ацетилирование сиаловых кислот в комплексе Гольджи
- Комплекс Гольджи. Распределение мембранных белков между эндоплазматическим ретикулумом и комплексом Гольджи
- МАТЕРИАЛЫ И МЕТОДЫ
- 1. Биологический материал
- 2. Поиск дифференциально экспрессирующихся генов Xenopus laevis
- 3. Клонирование гена Xcml
- 4. Секвенирование, анализ последовательности кДНК
- 5. Создание генных конструктов в экспрессионных плазмидных векторах
- 6. Анализ экспрессии гена Xcml в ходе развития Xenopus laevis
- 7. Анализ пространственной картины экспрессии генаХст^
- 8. Микроинъекции мРНК Xcml, синтезированной in vitro, в эмбрионы Xenopus
- 9. Культивирование клеток COS-1, экспрессия рекомбинантного белка и прижизненное окрашивание ядер
- 10. Фракционирование COS-1 клеток
- 11. Экспрессия белка в ооцитах Xenopus, фракционирование клеточного содержимого
- 12. Исследование внутриклеточной локализации Xcml с помощью конфокальной микроскопии
- 13. Гистологическая обработка и световая микроскопия
- РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЕ Клонирование нового гена Camello Xenopus laevis
- Новое генное семейство Camello
- Исследование области и времени экспрессии гена Xcml
- Исследование функций Xcml во время раннего развития Xenopus
- Исследование внутриклеточной локализации белка Xcml
- ВЫВОДЫ
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