Ингибиторный анализ роли процессинга ростовых факторов в индукции пролиферации культивируемых клеток
Диссертация
В качестве возможных ингибиторов были выбраны, главным образом, амины, что обусловлено наличием большого дискуссионного литературного материала относительно способности аминов блокировать кластеризацию, интернализацию и деградацию ЭФР. Однако, несмотря на то, что исследования велись ведущими научными коллективами (Maxfield et al., 1979аKing et al, 1981), полученные данные весьма противоречивы… Читать ещё >
Содержание
- Список сокращений
- 1. ВВЕДЕНИЕ
- 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Общие сведения о ростовых факторах
- 2. 2. Биологическое действие ЭФР
- 2. 3. ЭФР и его клеточный рецептор
- 2. 4. Процессинг ЭФР
- 2. 5. Исследования роли процессинга ЭФР в индукции пролиферации клеток
- 2. 6. Начальные реакции клеток на ЭФР
- 2. 7. Фосфорилирование уридина и его стимуляция при действии ЭФР
- 3. МАТЕРИАЛЫ И МЕТОДЫ
- 3. 1. Получение и очистка ЭФР, его производных и антител к ЭФР
- 3. 2. Методы культивирования и остановки пролиферации клеток
- 3. 3. Оценка уровня пролиферативной активности клеточных культур
- 3. 4. Изучение связывания, кластеризации интернализации
- ЭФР флуоресцентными методами
- 3. 5. Изучение связывания, интернализации и деградации 1251-ЭФР
- 3. 6. Приготовление и хранение растворов
- 3. 7. Поступление и распределение ДК в клетках ЗТЗ
- 3. 8. Измерение величины рН в лизосомах
- 3. 9. Измерение степени поляризации флуоресценции ДФГТ
- 3. 10. Измерение скорости транспорта и фосфорилирования уридина
- 3. 11. Описание кинетической модели поступления уридина в клетки
- 4. ЭКСПЕРИМЕНТАЛЬНАЯ ЧАСТ
- 4. 1. Влияние ингибиторов на индукцию пролиферации клеток
- 4. 2. Экспериментальное исследование кинетики поступления уридина в клетки
- 4. 3. Стимуляция фосфорилирования уридина РФ
- 4. 4. Действие ингибиторов пролиферации на активацию уридинкиназы
- 4. 5. Влияние аминов и других ингибиторов на кластеризацию, интернализацию и деградацию ЭФР
- 4. 6. Действие аминов на величину рН в лизосомах. .. 105 '4.7. Поступление и локализация ДК в клетках ЗТЗ. .. 112 4.8. Поляризация флуоресценции ДФГТ в мембранных фрагментах и интактных клетках
- 5. ОБСУЖДЕНИЕ
- 6. ВЫВОДЫ
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- В заключение хочу поблагодарить своего научного руководителя доктора биологических наук, профессора Николая Николаевича Никольского за постоянное внимание и помощь в работе.
- Выражаю искреннюю благодарность всем сотрудникам лаборатории физиологии клеточного цикла, где была сделана эта работа.