Роль основного стрессового белка млекопитающих (БТШ70) в защите миелоидных клеток от действия бактериального эндотоксина
Диссертация
Научная новизна. Впервые показан на клеточном уровне защитный эффект экзогенных БТШ70 (рекомбинантного и выделенного из сердечной мышцы верблюда) при действии эндотоксина. Установлено, что предварительное введение БТШ70 снижает генерацию АФК в различных типах миелоидных клеток, нормализует апоптоз нейтрофилов, ингибируемый при их стимуляции ЛПС, снижает ЛПС-индуцированный уровень экспрессии… Читать ещё >
Содержание
- Список сокращений
- ГЛАВА 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Определение и эпидемиология сепсиса
- 1. 2. Клетки врожденной иммунной системы и пути их взаимодействия с патогенами
- 1. 3. Липополисахарид грамотрицательных бактерий и его роль в развитии сепсиса
- 1. 3. 1. Строение молекулы липополисахарида и его формы
- 1. 3. 2. Взаимодействие бактериального эндотоксина с рецепторами клеток врожденной иммунной системы
- 1. 3. 3. Сигнальные каскады, запускаемые после узнавания ЛПС
- 1. 4. Характеристика семейства БТШ
- 1. 5. Использование БТШ70 в медицине
- 1. 6. Роль БТШ70 при воспалении и эндотоксемии
- 1. 6. 1. Действие эндогенного внутриклеточного БТШ70 при воспалении
- 1. 6. 2. Действие внеклеточного БТШ70 при воспалении
- 2. 1. Объекты исследования
- 2. 1. 1. Выделение нейтрофилов из периферической крови человека
- 2. 1. 1. 1. Определение жизнеспособности нейтрофилов
- 2. 1. 1. 2. Морфологический контроль клеток
- 2. 1. 2. Ведение культуры клеток мышиных макрофагов
- 2. 1. 3. Содержание животных
- 2. 1. 1. Выделение нейтрофилов из периферической крови человека
- 2. 2. Методы исследования
- 2. 2. 1. Выделение белков
- 2. 2. 1. 1. Выделение БТШ70 из сердечной мышцы верблюда
- 2. 2. 1. 2. Экспрессия и очистка рекомбинантного БТШ
- 2. 2. 2. Регистрация образования АФК нейтрофилами с помощью хемилюминесцентного метода
- 2. 2. 3. Регистрация апоптоза нейтрофилов
- 2. 2. 3. 1. Культивирование нейтрофилов для определения апоптоза
- 2. 2. 3. 2. Окрашивание клеток флуоресцентным зондом НоесЬз
- 2. 2. 4. Регистрация экспрессии р2-интегриновых рецепторов нейтрофилов
- 2. 2. 5. Измерение продукции оксида азота макрофагами (3774)
- 2. 2. 6. Диск-электрофорез белков в ПААГ с ДЦС-Иа (по Лэммли)
- 2. 2. 6. 1. Окрашивание гелей «Кумасси 11−250»
- 2. 2. 7. Двумерный электрофорез белков по О’Фарреллу
- 2. 2. 7. 1. Подготовка проб к двумерному электрофорезу
- 2. 2. 7. 2. Включение 338-метионина в белки
- 2. 2. 7. 3. Двумерное разделение белков в ПААГ
- 2. 2. 7. 4. Идентификация белков методом пептидного фингерпринта
- 2. 2. 7. 5. Подсчёт общего включения 358-метионина в белки
- 2. 2. 8. Моделирование экспериментального грамотрицательного сепсиса у крыс
- 2. 2. 9. Определение шаперонной активности БТШ70 методом ИФА
- 2. 2. 10. Статистическая обработка результатов
- 2. 2. 1. Выделение белков
- 3. 1. Исследование влияния рБТШ70 на выживаемость крыс в модели экспериментального грамотрицательного сепсиса
- 3. 2. Сравнение шаперонной активности БТШ70 млекопитающих из разных объектов
- 3. 3. Прекондиционирование с БТШ70 ослабляет ЛПС-индуцированный воспалительный ответ на клеточном уровне
- 3. 4. Прекондиционирование с БТШ70 значительно модифицирует ЛПС-индуцированную экспрессию Рг-интегриновых рецепторов нейтрофилов
- 3. 5. Предварительная обработка БТШ70 частично нормализует апоптоз нейтрофилов
- 3. 6. Прекондиционирование с препаратами БТШ70 ингибирует продукцию N
- 3. 7. Повышение температуры (ТШ) и экзогенный БТШ70 сходным образом ингибируют ЛПС-индуцированную продукцию N
- 3. 8. Анализ протеома клеток 1774 при комбинированной обработке рБТШ70, ЛПС и
Список литературы
- Брюсов П. Г., Костюченко А. Л. (1997). Воен-мед. Журнал 3, 28−34.
- Винокуров М. Г., Юринская М. М., Прохоренко И. Р., Грачев С. В. (2006). Действие различных хемотипов липополисахарида Е. coli на апоптоз и активацию PMN человека. Бюлл. эксп. биол. и мед. 147(8), 136−138.
- Владимиров Ю. А., Шерстнёв М. Р. (1989). Итоги науки и техники. Биофизика. М. ВИНИТИ 24, 176 С.
- Евдонин А. Л., Медведева Н. Д. (2009). Внеклеточный белок теплового шока 70 и его функции. Цитология 51(2), 130 137.
- Кетлинский С. А., Симбирцев А. С., Воробьев A.A. (1992) Эндогенные иммуномодуляторы. СПб.: Гиппократ.
- Коваленко Е. И., Власкин П. А., Каневский Л. М., Стрельникова Ю. И., Сапожников A.M. (2006). Влияние белков теплового шока 70 кДа на продукцию у интерферона натуральными киллерами человека. Докл. Акад. Наук 406(1), 121 — 124.
- Козлов В. К. (2006). Сепсис: этиология, иммунопатогенез, концепция современной иммунотерапии. СПб.: Диалект, 304 с.
- Маргулис Б.А., Гужова И. В. (2000). Белки стресса в эукариотической клетке. Цитология 42(4), 323 342.
- Мурашев А. Н., Медведев О. С., Давыдова С. А. Руководство по экспериментальной физиологии кровообращения. Саратов, 1992. -47 с.
- Новоселов С. С., Новоселова Т. В., Москалева О. С., Маргулис Б. А., Гужова И. В. (2004). Динамика шаперонных комплексов Hsp70 с белками-помощниками Hdj 1 и Bag 1 в реакции клеток эритролейкемии человека К562 на тепловой стресс. Цитология 46(7), 620−627.
- Пастухов Ю. Ф., Екимова И. В. (2005). Молекулярные, клеточные и системные механизмы протективной функции белка теплового шока 70 кДа. Молекулярная и клеточная нейробиология 2(2), 3−25.
- Afanasyev, V. N., Korol, В. A., Matylevich, N. P., Pechatnikov, V. A., and Umansky, S. R. (1993). The use of flow cytometry for the investigation of cell death. Cytometry 14, 603−609.
- Akira, S., and Takeda, K. (2004). Toll-like receptor signalling. Nat Rev Immunol 4, 499−511.
- Akira, S., Uematsu, S., and Takeuchi, 0. (2006). Pathogen recognition and innate immunity. Cell 124,783−801.
- Alves-Filho, J. C., de Freitas, A., Spiller, F., Souto, F. O., and Cunha, F. Q. (2008). The role of neutrophils in severe sepsis. Shock 30 Suppl 1, 3−9.
- Andra, J., Gutsmann, T., Garidel, P., and Brandenburg, K. (2006). Mechanisms of endotoxin neutralization by synthetic cationic compounds. J Endotoxin Res 12, 261−277.
- Andrades, M. E., Ritter, C., and Dal-Pizzol, F. (2009). The role of free radicals in sepsis development. Front Biosci (Elite Ed) 1, 277−287.
- Aneja, R., Odoms, K., Dunsmore, K., Shanley, T. P., and Wong, H. R. (2006). Extracellular heat shock protein-70 induces endotoxin tolerance in THP-1 cells. J Immunol 177, 7184−7192.
- Angus, D. C., and Wax, R. S. (2001). Epidemiology of sepsis: an update. Crit Care Med 29, S109−116.
- Anwar, S., and Whyte, M. K. (2007). Neutrophil apoptosis in infectious disease. Exp Lung Res 33, 519−528.
- Asea, A. (2003). Chaperokine-induced signal transduction pathways. Exerc Immunol Rev 9, 25−33.
- Asea, A., Kabingu, E., Stevenson, M. A., and Calderwood, S.' K. (2000a). HSP70 peptidembearing and peptide-negative preparations act as chaperokines. Cell Stress Chaperones 5,425−431.
- Basu, S., Binder, R. J., Ramalingam, T., and Srivastava, P. K. (2001). CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin. Immunity 14, 303
- Bausero, M. A., Gastpar, R., Multhoff, G., and Asea, A. (2005). Alternative mechanism by which IFN-gamma enhances tumor recognition: active release of heat shock protein 72. J Immunol 175, 2900−2912.
- Becker, T., Hartl, F. U., and Wieland, F. (2002). CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J Cell Biol 158, 1277−1285.
- Benjamini E., Sunshine G., Leskowitz S. (1996) Immunology, a short course. WILEYLISS, New York, 451.
- Beutler, B., Hoebe, K., Du, X., and Ulevitch, R. J. (2003). How we detect microbes and respond to them: the Toll-like receptors and their transducers. J Leukoc Biol 74, 479 485.
- Beutler, B., Jiang, Z., Georgel, P., Crozat, K., Croker, B., Rutschmann, S., Du, X., and Hoebe, K. (2006). Genetic analysis of host resistance: Toll-like receptor signaling and immunity at large. Annu Rev Immunol 24, 353−389.
- Binder, R. J., Harris, M. L., Menoret, A., and Srivastava, P. K. (2000). Saturation, competition, and specificity in interaction of heat shock proteins (hsp)-gp96, hsp90, and hsp70 with CDllb+ cells. J Immunol 165, 2582−2587.
- Blachere, N. E., Udono, H., Janetzki, S., Li, Z., Heike, M., and Srivastava, P. K. (1993). Heat shock protein vaccines against cancer. J Immunother Emphasis Tumor Immunol 14, 352−356.
- Bomsztyk, K., Denisenko, O., and Ostrowski, J. (2004). hnRNP K: one protein multiple processes. Bioessays 26, 629−638.
- Bone, R. C. (1995). Sepsis and controlled clinical trials: the odyssey. Crit Care Med 23, 11 651 166.
- Brandenburg, K., and Wiese, A. (2004). Endotoxins: relationships between structure, function, and activity. Curr Top Med Chem 4, 1127−1146.
- Brodsky, J. L., and Chiosis, G. (2006). Hsp70 molecular chaperones: emerging roles in human disease and identification of small molecule modulators. Curr Top Med' Chem 6, 1215−1225.
- Broquet, A. H., Thomas, G., Masliah, J., Trugnan, G., and Bachelet, M. (2003). Expression of the molecular chaperone Hsp70 in detergent-resistant microdomains correlates with its membrane delivery and release. J Biol Chem 278, 21 601−21 606.
- Brown, G. D. (2006). Dectin-1: a signalling non-TLR pattern-recognition receptor. Nat Rev Immunol 6, 33−43.
- Bruemmer-Smith, S., Stuber, F., and Schroeder, S. (2001). Protective functions of intracellular heat-shock protein (HSP) 70-expression in patients with severe sepsis. Intensive Care Med 27, 1835−1841.
- Burchardi, H., and Schneider, H. (2004). Economic aspects of severe sepsis: a review of intensive care unit costs, cost of illness and cost effectiveness of therapy. Pharmacoeconomics 22, 793−813.
- Calderwood, S. K., Mambula, S. S., and Gray, P. J., Jr. (2007a). Extracellular heat shock proteins in cell signaling and immunity. AnnN Y Acad Sci 1113, 28−39.
- Calderwood, S. K., Theriault, J., Gray, P. J., and Gong, J. (2007b). Cell surface receptors for molecular chaperones. Methods 43, 199−206.
- Campisi, J., and Fleshner, M. (2003). Role of extracellular HSP72 in acute stress-induced potentiation of innate immunity in active rats. J Appl Physiol 94, 43−52.
- Campisi, J., Leem, T. H., and Fleshner, M. (2003). Stress-induced extracellular Hsp72 is a functionally significant danger signal to the immune system. Cell Stress Chaperones 8, 272−286.
- Caroff, M., and Karibian, D. (2003). Structure of bacterial lipopolysaccharides. Carbohydr Res 338, 2431−2447.
- Carper, S. W., Duffy, J. J., and Gerner, E. W. (1987). Heat shock proteins in thermotolerance and other cellular processes. Cancer Res 47, 5249−5255.
- Chang, L., and Karin, M. (2001). Mammalian MAP kinase signalling cascades. Nature 410, 37−40.
- Chen, H., Wu, Y., Zhang, Y., Jin, L., Luo, L., Xue, B., Lu, C., Zhang, X., and Yin, Z. (2006). Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination. FEBS Lett 580, 3145−3152.
- Clayton, A., Turkes, A., Navabi, H., Mason, M. D., and Tabi, Z. (2005). Induction of heat shock proteins inB-cell exosomes. J Cell Sci 118, 3631−3638.
- Corradin, S. B., Mauel, J., Gallay, P., Heumann, D., Ulevitch, R. J., and Tobias, P. S. (1992). Enhancement of murine macrophage binding of and response to bacterial lipopolysaccharide (LPS) by LPS-binding protein. J Leukoc Biol 52, 363−368.
- Cross, A. S., and Opal, S. M. (2003). A new paradigm for the treatment of sepsis: is it time to consider combination therapy? Ann Intern Med 138, 502−505.
- Currie, R. W., Karmazyn, M., Kloc, M., and Mailer, K. (1988). Heat-shock response is associated with enhanced postischemic ventricular recovery. Circ Res 63, 543−549.
- Cuschieri, J., Billigren, J., and Maier, R. V. (2006). Endotoxin tolerance attenuates LPS-induced TLR4 mobilization to lipid rafts: a condition reversed by PKC activation. J Leukoc Biol 80, 1289−1297.
- Deguchi, Y., and Kishimoto, S. (1990). Spontaneous activation of heat shock protein gene transcription in peripheral blood mononuclear cells of individuals with active systemic lupus erythematosus. Clin Rheumatol 9, 182−185.
- Dokladny, K., Lobb, R., Wharton, W., Ma, T. Y., and -Moseley, P. L. (2010). LPS-induced ' cytokine levels are repressed by elevated’expression of HSP70 in rats: possible role of NF-kappaB. Cell Stress Chaperones 15, 153−163.
- Ensor, J. E., Wiener, S. M., McCrea, K. A., Yiscardi, R. M., Crawford, E. K., and Hasday, J. D. (1994). Differential effects of hyperthermia on macrophage interleukin-6 and tumor necrosis factor-alpha expression. Am J Physiol 266, C967−974.
- Fleshner, M., Campisi, J., Amiri, L., and Diamond, D. M. (2004). Cat exposure induces both intra- and extracellular Hsp72: the role of adrenal hormones. Psychoneuroendocrinology 29, 1142−1152.
- Floto, R. A., MacAry, P. A., Boname, J. M., Mien, T. S., Kampmann, B., Hair, J. R., Huey, O. S., Houben, E. N., Pieters, J., Day, C., et al. (2006). Dendritic cell stimulation by mycobacterial Hsp70 is mediated through CCR5. Science 314,.454−458.
- Freeman, B. C., and Morimoto, R. I. (1996). The human cytosolic molecular chaperones hsp90, hsp70 (hsc70) and hdj-1 have distinct roles in recognition of a non-native proteinand protein refolding. EMBO J 15, 2969−2979.
- Freudenberg, N., Freudenberg, M. A., Guzman, J., Mittermayer, C., Bandara, K., and Galanos, C. (1984). Identification of endotoxin-positive cells in the rat lung during shock. Virchows Arch A Pathol Anat Histopathol 404, 197−211.
- Frydman, J. (2001). Folding of newly translated proteins in vivo: the role of molecular chaperones. Annu Rev Biochem 70, 603−647.
- Fujihara, M., Muroi, M., Tanamoto, K., Suzuki, T., Azuma, H., and Ikeda, H. (2003). Molecular mechanisms of macrophage activation and deactivation by lipopolysaccharide: roles of the receptor complex. Pharmacol Ther 100, 171−194.
- Fujihara, Y., Bogard, W. C., Lei, M. G., Daddona, P. E., and Morrison, D. C. (1993). Monoclonal anti-lipid A IgM’antibodies HA-1A and E-5 recognize distinct epitopes on lipopolysaccharide and lipid A. J Infect Dis 168- 1429−1435.
- Gebauer, M., Zeiner, M., and Gehring, U. (1998). Interference between proteins Hap46 and Hop/p60, which bind to different domains of the molecular chaperone hsp70/hsc70. Mol Cell Biol 18, 6238−6244.
- Ghosh, S., May, Mc. J., and Kopp, E. B. (1998). NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol 16, 225−260.
- Guzhova, I., Kislyakova, K., Moskaliova, O., Fridlanskaya, I., Tytell, M, Cheetham, M., and
- Margulis, В. (2001). In vitro studies show that Hsp70 can be released by glia and that exogenous Hsp70 can enhance neuronal stress tolerance. Brain Res 914, 66−73.
- Guzhova, I., and Margulis, B. (2006). Hsp70 chaperone as a survival factor in cell pathology. Int Rev Cytol 254, 101−149.
- Hagiwara, S., Iwasaka, H., Matsumoto, S., and Noguchi, T. (2007). Changes in cell culture temperature alter release of inflammatory mediators in murine macrophagic RAW264.7 cells. Inflamm Res 56, 297−303.
- Hamilton, K. L., Gupta, S., and Knowlton, A. A. (2004). Estrogen and regulation of heat shock protein expression in female cardiomyocytes: cross-talk with NF kappa В signaling. J Mol Cell Cardiol 36, 577−584.
- Hampton, R. Y., Golenbock, D. Т., Penman, M., Krieger, M., and Raetz, C. R. (1991). Recognition and plasma clearance of endotoxin by scavenger receptors. Nature 352, 342−344.
- Hashimoto, C., Hudson, K. L., and Anderson, К. V. (1988). The Toll gene of Drosophila, required for dorsal-ventral embryonic polarity, appears to encode a transmembrane protein. Cell 52, 269−279.
- Haurum, J. S., Thiel, S., Jones, I. M., Fischer, P. В., Laursen, S. В., and Jensenius, J. C. (1993). Complement activation upon binding of mannan-binding protein to HIV envelope glycoproteins. AIDS 7, 1307−1313.
- He, H., Chen, C., Xie, Y., Asea, A., and Calderwood, S. K. (2000). HSP70 and heat shock factor 1 cooperate to repress Ras-induced transcriptional activation of the c-fos gene. Cell Stress Chaperones 5, 406−411.
- Herlaar, E., and Brown, Z. (1999). p38 МАРК signalling cascades in inflammatory disease. MoliMed Today 5, 439−447.
- Hofrnan, P. (2004). Molecular regulation of neutrophil apoptosis and potential targets for therapeutic strategy against the inflammatory process. Curr Drug Targets Inflamm Allergy 3, 1−9.
- Horng, T., Barton, G. M., and Medzhitov, R. (2001). TIRAP: an adapter molecule in the Toll ' signaling pathway. Nat Immunol 2, 835−841.
- Hotchkiss, R., Nunnally, I., Lindquist, S., Taulien, J., Perdrizet, G., and Karl, I. (1993). Hyperthermia protects mice against the lethal effects of endotoxin. Am J Physiol 265, R1447−1457.
- Huber, M., Kalis, C., Keck, S., Jiang, Z., Georgel, P., Du, X., Shamel, L., Sovath, S., Mudd, S., Beutler, B., et al. (2006). R-form LPS, the master key to the activation ofTLR4/MD-2-positive cells. Eur J Immunol 36, 701−711.
- Jaattela, M. (1993). Overexpression of major heat shock protein hsp70 inhibits tumor necrosis factor-induced activation of phospholipase A2. J Immunol 151, 4286−4294.
- Jaattela, M., Wissing, D., Bauer, P. A., and Li, G. C. (1992). Major heat shock protein hsp70 protects tumor cells from tumor necrosis factor cytotoxicity. EMBO J 11, 3507−3512.
- Janssens, S., and Beyaert, R. (2003). Role of Toll-like receptors in pathogen recognition. Clin
- Microbiol Rev 16, 637−646.
- Jiang, W., Sun, R., Wei, H., and Tian, Z. (2005a). Toll-like receptor 3 ligand attenuates LPS-induced liver injury by down-regulation of toll-like receptor 4 expression on macrophages. Proc Natl Acad Sci U S A 102, 17 077−17 082.
- Jiang, Z., Georgel- P., Du, X., Shamel, L., Sovath, S., Mudd, S., Huber, M., Kalis, C., Keck, S., Galanos, C., et al. (2005b). CD14 is required for MyD88-independent LPS signaling. Nat Immunol 6, 565−570.
- Kagan, J. C., and Medzhitov, R. (2006). Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling. Cell 125, 943−955.
- Kantari, C., Pederzoli-Ribeil, M., and Witko-Sarsat, V. (2008). The role of neutrophils and monocytes in innate immunity. Contrib Microbiol 15, 118−146.
- Keel, M., Ungethum, U., Steckholzer, U., Niederer, E., Hartung, T., Trentz, O., and Ertel, W. (1997). Interleukin-10 counterregulates proinflammatory cytokine-induced inhibition of neutrophil apoptosis during severe sepsis. Blood 90- 3356−3363.
- Kettritz, R., Choi, M., Salanova, B., Wellner, M., Rolle, S., and Luft, F. C. (2006). Fever-like temperatures affect neutrophil NF-kappaB signaling, apoptosis, and ANCA-antigen expression. J Am Soc Nephrol 17, 1345−1353.
- Kim, J. Y., and Ozato, K. (2009). The sequestosome l/p62 attenuates cytokine gene expression in activated macrophages by inhibiting IFN regulatory factor 8 and TNF receptor-associated factor 6/NF-kappaB activity. J Immunol 182, 2131−2140.
- Kim, S., Nollen, E. A., Kitagawa, K., Bindokas, V. P., and Morimoto, R. I. (2002). Polyglutamine protein aggregates are dynamic. Nat Cell Biol 4, 826−831.
- Kitchens, R. L., Wolfbauer, G., Albers, J. J., and Munford, R. S. (1999). Plasma lipoproteins promote the release of bacterial lipopolysaccharide from the monocyte cell surface. J Biol Chem 274, 34 116−34 122.
- Mackey, M. F., Barth, R. J., Jr., and Noelle, R. J. (1998). The role of CD40/CD154 interactions in the priming, differentiation, and effector function of helper and cytotoxic T cells. J Leukoc Biol 63, 418−428.
- Malhotra, R., and Bird, M. I. (1997). L-Selectin—a signalling receptor for lipopolysaccharide. ChemBiol 4, 543−547.
- Malhotra, R., Priest, R., and Bird, M. I. (1996). Role for L-selectin in lipopolysaccharide-induced activation of neutrophils. Biochem J 320 (Pt 2), 589−593.
- Malhotra, R., Priest, R., Foster, M. R., and Bird, M. I. (1998). P-selectin binds to bacterial lipopolysaccharide. Eur J Immunol 28, 983−988.
- Mambula, S. S., and Calderwood, S. K. (2006). Heat shock protein 70 is secreted from tumor cells by a nonclassical pathway involving lysosomal endosomes. J Immunol 177, 78 497 857.
- Mambula, S. S., Stevenson, M. A., Ogawa, K., and Calderwood, S. K. (2007). Mechanisms for Hsp70 secretion: crossing membranes without a leader. Methods 43, 168−175.
- Martin, G. S., Mannino, D. M., Eaton, S., and Moss, M. (2003). The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 348,1546−1554.
- Matzinger, P. (2002). The danger model: a renewed sense of self. Science 296, 301−305.
- Mayer, M. P., Brehmer, D., Gassier, C. S., and Bukau, B. (2001). Hsp70 chaperone machines. Adv Protein Chem 59, 1−44.
- Medzhitov, R. (2001). Toll-like receptors and innate immunity. Nat Rev Immunol 1, 135−145.
- Medzhitov, R., and Janeway, C. A, Jr. (1997a). Innate immunity: impact on the adaptive immune response. Curr Opin Immunol 9, 4−9.
- Medzhitov, R., and Janeway, C. A., Jr. (1997b). Innate immunity: the virtues of a nonclonal system of recognition. Cell 91, 295−298.
- Medzhitov, R., Preston-Hurlburt, P., and Janeway, C. A., Jr. (1997). A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 388, 394 397.
- Mehta, H. B., Popovich, B. K., and Dillmann, W. H. (1988). Ischemia induces changes in the level of mRNAs coding for stress protein 71 and creatine kinase M. Circ Res 63, 512 517.
- Melnick, J., Aviel, S., and Argon, Y. (1992). The endoplasmic reticulum stress protein
- GRP94, in addition to BiP, associates with unassembled immunoglobulin chains. J Biol Chem 267,21 303−21 306.
- Millar, D. G., Garza, K. M., Odermatt, B., Elford, A. R., Ono, N., Li, Z., and Ohashi, P. S. (2003). Hsp70 promotes antigen-presenting cell function and converts T-cell tolerance to autoimmunity in vivo. Nat Med 9,1469−1476.
- Minota, S., Cameron, B., Welch, W. J., and Winfield, J. B. (1988). Autoantibodies to the constitutive 73-kD member of the hsp70 family of heat shock proteins in systemic lupus erythematosus. J Exp Med 168, 1475−1480.
- Montesano Gesualdi, N., Chirico, G., Pirozzi, G., Costantino, E., Landriscina, M., and Esposito, F. (2007). Tumor necrosis factor-associated protein 1 (TRAP-1) protects cells from oxidative stress and apoptosis. Stress 10, 342−350.
- Morimoto, R. I. (1998). Regulation of the heat shock transcriptional response: cross talk between a family of heat shock factors, molecular chaperones, and negative regulators. Genes Dev 12, 3788−3796.
- Morimoto R, Tissieres A, Georgopoulos C. (1990) The Stress Response, Function of the Proteins, and Perspectives. Stress Proteins in Biology and Medicine. Cold Spring Harbor Laboratory Press. 1—32.
- Muchowski, P. J., Schaffar, G., Sittler, A., Wanker, E. E., Hayer-Hartl, M. K., and Hartl, F. U. (2000). Hsp70 and hsp40 chaperones can inhibit self-assembly of polyglutamine proteins into amyloid-like fibrils. ProcNatl Acad SciU S A 97, 7841−7846.
- Murry, C. E., Jennings, R. B., and Reimer, K. A. (1986). Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 74, 1124−1136.
- Nagai, Y., Akashi, S., Nagafuku, M., Ogata, M., Iwakura, Y., Akira, S., Kitamura, T., Kosugi, A., Kimoto, M., and Miyake, K. (2002). Essential role of MD-2 in LPS responsiveness and TLR4 distribution. Nat Immunol 3, 667−672.
- Nauseef, W. M. (2007). How human neutrophils kill and degrade microbes: an integrated view. Immunol Rev 219, 88−102.
- Nelson, R. J., Ziegelhoffer, T., Nicolet, C., Werner-Washburne, M., and Craig, E. A. (1992). The translation machinery and 70 kd heat shock protein cooperate in protein synthesis. Cell 71, 97−105.
- Neupert, W., Hartl, F. U., Craig, E. A., and Pfanner, N. (1990). How do polypeptides cross the mitochondrial membranes? Cell 63, 447−450.
- Njemini, R., Lambert, M., Demanet, C., and Mets, T. (2003). Elevated serum heat-shock protein 70 levels in patients with acute infection: use of an optimized enzyme-linked immunosorbent assay. Scand J Immunol 58, 664−669.
- Nollen, E. A., and Morimoto, R. I. (2002). Chaperoning signaling pathways: molecular chaperones as stress-sensing 'heat shock' proteins. J Cell Sci 115, 2809−2816.
- O’Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250, 4007−4021.
- O’Neill, L. A., and Bowie, A. G. (2007). The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling. Nat Rev Immunol7, 353−364.
- Opal, S. M. (2007). The host response to endotoxin, antilipopolysaccharide strategies, and the management of severe sepsis. Int J Med Microbiol 297, 365−377.
- Osawa, Y., Lee, H. T., Hirshman, C. A., Xu, D., and Emala, C. W. (2006). Lipopolysaccharide-induced sensitization of adenylyl cyclase activity in murine macrophages. Am J Physiol Cell Physiol 290, C143−151.
- Pabst, M. J., Pabst, K. M, Handsman, D. B., Beranova-Giorgianni, S., and Giorgianni, F. (2008). Proteome of monocyte priming by lipopolysaccharide, including changes in interleukin-lbeta and leukocyte elastase inhibitor. Proteome Sci 6, 13.
- Panjwani, N. N., Popova, L., and Srivastava, P. K. (2002). Heat shock proteins gp96 and hsp70 activate the release of nitric oxide by APCs. J Immunol 168, 2997−3003.
- Parrillo, J. E. (1993). Pathogenetic mechanisms of septic shock. N Engl J Med 328, 14 711 477.
- Petrak, J., Ivanek, R., Toman, O., Cmejla, R., Cmejlova, J., Vyoral, D., Zivny, J., and Vulpe, C. D. (2008). Deja vu in proteomics. A hit parade of repeatedly identified differentially expressed proteins. Proteomics 8, 1744−1749.
- Pierce, S. K. (1994). Molecular chaperones in the processing and presentation of antigen to helper T cells. Experientia 50, 1026−1030.
- Pockley, A. G., Muthana, M., and Calderwood, S. K. (2008). The dual immunoregulatory roles of stress proteins. Trends Biochem Sci 33, 71−79.
- Pockley, A. G., Shepherd, J., and Corton, J. M. (1998). Detection of heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal individuals. Immunol Invest 27, 367−377.
- Poltorak, A., He, X., Smirnova, I., Liu, M. Y., Van Huffel, C., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C., et al. (1998). Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282, 2085−2088.
- Power, C., Fanning, N., and Redmond, H. P. (2002). Cellular apoptosis and organ injury in sepsis: a review. Shock 18, 197−211.
- Prestes-Carneiro, L. E., Shio, M. T., Fernandes, P. D., and Jancar, S. (2007). Cross-regulation of iNOS and COX-2 by its products in murine macrophages under stress conditions. Cell Physiol Biochem 20, 283−292.
- Radons, J., and Multhoff, G. (2005). Immunostimulatory functions of membrane-bound and exported heat shock protein 70. Exerc Immunol Rev 11, 17−33.
- Randow, F., and' Seed, B. (2001). Endoplasmic reticulum chaperone gp96 is required for innate immunity but not cell viability. Nat Cell Biol 3, 891−896.
- Raposo, G., Nijman, H. W., Stoorvogel, W., Liejendekker, R., Harding, C. V., Melief, C. J., and Geuze, H. J. (1996). B lymphocytes secrete antigen-presenting vesicles. J Exp Med 183, 1161−1172.
- Raska, M., and Weigl, E. (2005). Heat shock proteins in autoimmune diseases. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149, 243−249.
- Ribeiro, S. P., Villar, J., Downey, G. P., Edelson, J. D., and Slutsky, A. S. (1994). Sodium arsenite induces heat shock protein-72 kilodalton expression in the lungs and protects rats against sepsis. Crit Care Med 22, 922−929.
- Riedemann, N. C., Guo, R. F., and Ward, P. A. (2003a). The enigma of sepsis. J Clin Invest 112, 460−467.
- Riedemann, N. C., Guo, R. F., and Ward, P. A. (2003b). Novel strategies for the treatment of sepsis. Nat Med 9, 517−524.
- Rifkin, I. R., Leadbetter, E. A., Busconi, L., Viglianti, G., and Marshak-Rothstein, A. (2005). Toll-like receptors, endogenous ligands, and systemic autoimmune disease. Immunol Rev 204, 27−42.
- Rittirsch, D., Flierl, M. A., and Ward, P. A. (2008). Harmful molecular mechanisms in sepsis. Nat Rev Immunol 8, 776−787.
- Ryan, A. J., Flanagan, S. W., Moseley, P. L., and Gisolfi, C. V. (1992). Acute heat stress protects rats against endotoxin shock. J Appl Physiol 73, 1517−1522.
- Salomao, R., Martins, P. S., Brunialti, M. K., Fernandes Mda, L., Martos, L. S., Mendes, M.
- E., Gomes, N. E., and Rigato, O. (2008). TLR signaling pathway in patients with sepsis. Shock 30 Suppl 1, 73−77.
- Samali, A., Cai, J., Zhivotovsky, B., Jones, D. P., and Orrenius, S. (1999). Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and HsplO in the mitochondrial fraction of jurkat cells. EMBO J 18, 2040−2048.
- Sapozhnikov, A. M., Gusarova, G. A., Ponomarev, E. D., and Telford, W. G. (2002). Translocation of cytoplasmic HSP70 onto the surface of EL-4 cells during apoptosis. Cell Prolif 35, 193−206.
- Sato, S., Sanjo, H., Takeda, K., Ninomiya-Tsuji, J., Yamamoto, M., Kawai, T., Matsumoto, K., Takeuchi, O., and Akira, S. (2005). Essential function for the kinase TAK1 in innate and adaptive immune responses. Nat Immunol 6, 1087−1095.
- Sawamura, T., Kume, N., Aoyama, T., Moriwaki, H., Hoshikawa, H., Aiba, Y., Tanaka, T., Miwa, S., Katsura, Y., Kita, T., and Masaki, T. (1997). An endothelial receptor for oxidized low-density lipoprotein Nature 386, 73−77.
- Schell, M. T., Spitzer, A. L., Johnson, J. A., Lee, D., and Harris, H. W. (2005). Heat shock inhibits NF-kB activation in a dose- and time-dependent manner. J Surg Res 129, 90−93.
- Schumann, R. R., Leong, S. R., Flaggs, G. W., Gray, P. W., Wright, S. D., Mathison, J. C., Tobias, P. S., and Ulevitch, R. J. (1990). Structure and function of lipopolysaccharide binding protein. Science 249, 1429−1431.
- Schutt, C., Schilling, T., Grunwald, U., Schonfeld, W., and Kruger, C. (1992). Endotoxin-neutralizing capacity of soluble CD14. Res Immunol 143, 71−78.
- Serhan, C. N., and Savill, J. (2005). Resolution of inflammation: the beginning programs the end. Nat Immunol 6, 1191 -1197.
- Shanley TP, Hallstrom C, Wong HR. (2006) Sepsis. In: Fuhrman BP, Zimmerman JJ, editors. Pediatric Critical Care Medicine. St. Louis: Mosby 3, 1474−1493.
- Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V., and Mann, M. (2006). In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat Protoc 1, 28 562 860.
- Shin, H. J., Lee, H., Park, J. D., Hyun, H. C" Sohn, H. O., Lee, D. W., and Kim, Y. S. (2007). Kinetics of binding of LPS to recombinant CD 14, TLR4, and MD-2 proteins. Mol Cells 24, 119−124.
- Singleton, K. D., and Wischmeyer, P. E. (2006). Effects of HSP70.1/3 gene knockout on acute respiratory distress syndrome and the inflammatory response following sepsis. Am J Physiol Lung Cell Mol Physiol 290, L956−961.
- Stefanova, I., Horejsi, V., Ansotegui, I. J., Knapp, W., and Stockinger, H. (1991). GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science 254, 1016−1019.
- Stoorvogel, W., Kleijmeer, M. J., Geuze, H. J., and Raposo, G. (2002). The biogenesis and functions of exosomes. Traffic 3, 321−330.
- Swearingen, K. E., Loomis, W. P., Zheng, M., Cookson, B. T., and Dovichi, N. J. Proteomic profiling of lipopolysaccharide-activated macrophages by isotope coded affinity tagging. J Proteome Res 9, 2412−2421.
- Terlecky, S. R., Chiang, H. L., Olson, T. S., and Dice,.J. F. (1992). Protein and peptide binding and stimulation-of in vitro lysosomal proteolysis by the 73-kDa heat shockcognate protein. J Biol Chem 267, 9202−9209.
- Teshima, S., Rokutan, K., Takahashi, M., Nikawa, T., and Kishi, K. (1996). Induction of heat shock proteins and their possible roles in macrophages during activation by macrophage colony-stimulating factor. Biochem J 315 (Pt 2), 497−504.
- Theriault, J. R., Mambula, S. S., Sawamura, T., Stevenson, M. A., and Calderwood, S. K. (2005). Extracellular HSP70 binding to surface receptors present on antigen presenting cells and endothelial/epithelial cells. FEBS Lett 579, 1951−1960.
- Tissieres, A., Mitchell, H. K., and Tracy, U. M. (1974). Protein synthesis in salivary glands of Drosophila melanogaster: Relation to chromosome puffs. J Mol Biol 85, 389−398.
- Tobias, P. S., Soldau, K., and Ulevitch, R. J. (1986). Isolation of a lipopolysaccharide-binding acute phase reactant from rabbit serum. J Exp Med 164, 777−793.
- Tobias, P. S., and Ulevitch, R. J. (1993). Lipopolysaccharide binding protein and CD14 in LPS dependent macrophage activation. Immunobiology 187, 227−232.
- Triantafilou, K., Triantafilou, M., and Dedrick, R. L. (2001). Interactions of bacterial lipopolysaccharide and peptidoglycan with a 70 kDa and an 80 kDa protein on the cell surface of CD 14+ and CD 14- cells. Hum Immunol 62, 50−63.
- Triantafilou, Mr, and Triantafilou, K. (2002). Lipopolysaccharide recognition: CD 14, TLRs and the LPS-activation cluster. Trends Immunol 23, 301−304.
- Triantafilou, M., and Triantafilou, K. (2005). The dynamics of LPS recognition: complex orchestration of multiple receptors. J Endotoxin Res 11,5−11.
- Tsan, M. F., and Gao, B. (2004a). Cytokine function of heat shock proteins. Am J Physiol Cell Physiol 286, C739−744.
- Tsan, M. F., and" Gao, B. (2004b). Heat shock protein and innate immunity. Cell Mol Immunol 1, 274−279.
- Tsan, M. F., and Gao, B. (2009). Heat shock proteins and immune system. J Leukoc Biol 85, 905−910.
- Tytell, M., and Hooper, P. L. (2001). Heat shock proteins: new keys to the development of cytoprotective therapies. Expert Opin Ther Targets 5, 267−287.
- Ulmer, A.J. et al. (2002). Lipopolysaccharide: structure, bioactivity, receptors, and signaltransduction. Trends in Glycoscience and Glycotechnology 14, 53−68.
- Underhill, D. M., and Ozinsky, A. (2002). Phagocytosis of microbes: complexity in action. Annu Rev Immunol 20, 825−852.
- Van Molle, W., Wielockx, B., Mahieu, T., Takada, M., Taniguchi, T., Sekikawa, K., and Libert, C. (2002). HSP70 protects against TNF-induced lethal inflammatory shock. Immunity 16, 685−695.
- Villar, J., Ribeiro, S. P., Mullen, J. B., Kuliszewski, M., Post, M., and Slutsky, A. S. (1994). Induction of the heat shock response reduces mortality rate and organ damage in a sepsis-induced acute lung injury model. Crit Care Med 22, 914−921.
- Viriyakosol, S., Tobias, P. S., Kitchens, R. L., and Kirkland, T. N. (2001). MD-2 binds to bacterial lipopolysaccharide. J Biol Chem 276, 38 044−38 051.
- Visintin, A., Mazzoni, A., Spitzer, J. A., and Segal, D. M. (2001a). Secreted MD-2 is a large polymeric protein that efficiently confers lipopolysaccharide sensitivity to Toll-like receptor 4. Proc Natl Acad Sci U S A 98, 12 156−12 161.
- Visintin, A., Mazzoni, A., Spitzer, J. H., Wyllie, D. H., Dower, S. K., and Segal, D. M. (2001b). Regulation of Toll-like receptors in human monocytes and dendritic cells. J Immunol 166, 249−255.
- Wang, Y., Li, C., Wang, X., Zhang, J., and Chang, Z. (2002). Heat shock response inhibits1.-18 expression through the JNK pathway in murine peritoneal macrophages. Biochem Biophys Res Commun 296, 742−748.
- Wawrzynow, A., and Zylicz, M. (1995). Divergent effects of ATP on the binding of the DnaK and DnaJ chaperones to each other, or to their various native and denatured protein substrates. J Biol Chem 270, 19 300−19 306.
- Welch, W. J. (1993). Heat shock proteins functioning as molecular chaperones: their roles in normal and stressed cells. Philos Trans R Soc Lond B Biol Sci 339, 327−333.
- Welch, W. J., and Suhan, J. P. (1986). Cellular and biochemical events in mammalian cells during and after recovery from physiological stress. J Cell Biol 103, 2035−2052.
- Wesche-Soldato, D. E., Swan, R. Z., Chung, C. S., and Ayala, A. (2007). The apoptotic pathway as a therapeutic target in sepsis. Curr Drug Targets 8, 493−500.
- Wong, H. R. (1998). Potential protective role of the heat shock response in sepsis. New Horiz 6, 194−200.
- Worthen, G. S., Avdi, N., Vukajlovich, S., and Tobias, P. S. (1992). Neutrophil adherence induced by lipopolysaccharide in vitro. Role of plasma component interaction with lipopolysaccharide. J Clin Invest 90, 2526−2535.
- Wright, S. D., and Jong, M. T. (1986). Adhesion-promoting receptors on human macrophages recognize Escherichia coli by binding to lipopolysaccharide. J Exp Med 164, 18 761 888.
- Wright, S. D., Ramos, R. A., Tobias, P. S., Ulevitch, R. J., and Mathison, J. C. (1990). CD14, a receptor for complexesiof lipopolysaccharide (LPS) and LPS binding protein. Science 249, 1431−1433.
- Wygrecka, M., Marsh, L. M., Morty, R. E., Henneke, I., Guenther, A., Lohmeyer, J., Markart, P., and Preissner, K. T. (2009). Enolase-l promotes plasminogen-mediated recruitmentof monocytes to the acutely inflamed lung. Blood 113, 5588−5598.
- Yamamoto, M., Sato, S., Hemmi, H., Hoshino, K., Kaisho, T., Sanjo, H., Takeuchi, O., Sugiyama, M., Okabe, M., Takeda, K., and Akira, S. (2003). Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway. Science 301, 640−643.
- Zhang, G., and Ghosh, S. (2000). Molecular mechanisms of NF-kappaB activation induced by bacterial lipopolysaccharide through Toll-like receptors. J Endotoxin Res 6, 453−457.
- Zhao, Y., Wang, W., and Qian, L. (2007). Hsp70 may protect cardiomyocytes from stress-induced injury by inhibiting Fas-mediated apoptosis. Cell Stress Chaperones 12, 83−95.
- Zheng, H., Nagaraja, G. M., Kaur, P., Asea, E. E., and Asea, A. (2010). Chaperokine function of recombinant Hsp72 produced in insect cells using a baculovirus expression system is retained. J Biol Chem 285, 349−356.
- Zheng, L., He, M., Long, M., Blomgran, R., and Stendahl, O. (2004). Pathogen-induced apoptotic neutrophils express heat shock proteins and elicit activation of human macrophages. J Immunol 173, 6319−6326.
- Zheng, Y., Im, C. N., and Seo, J. S. (2006). Inhibitory effect of Hsp70 on angiotensin II-induced vascular smooth muscle cell hypertrophy. Exp Mol Med 38, 509−518.1. БЛАГОДАРНОСТИ
- Особая благодарность моему мужу Рожкову Николаю за понимание и поддержку на всем протяжении выполнения диссертационной работы.
- Кроме того, выражаю искреннюю признательность Зеленцовой Е. С., Мяснянкиной E.H., Шостак Н. Г., Гарбузу Д. Г., Юшеновой И. А. за чуткое отношение, понимание и создание в лаборатории доброжелательной дружеской обстановки, располагающей к плодотворной работе.