Синтез олигонуклеотидных конъюгатов с помощью реакции [3+2]-диполярного циклоприсоединения и их использование в конструировании ДНК-наноструктур
Диссертация
Существует ряд способов получения модифицированных олигонуклеотидов. Постсинтетические методы, связанные с введением реакционноспособной группы на стадии твердофазного олигонуклеотидного синтеза и ее последующей модификацией, предпочтительны для синтеза олигонуклеотидных конъюгатов с фрагментами, недостаточно стабильными в условиях олигонуклеотидного синтеза (ОНС) и деблокирования. Такими… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- САМОСОБИРАЮЩИЕСЯ ДНК-НАНОСТРУКТУРЫ (обзор литературы)
- 1. Разветвленные наноструктуры, полученные из немодифицированной ДНК
- 2. Дискретные ДНК-наноструктуры
- 3. Периодические ДНК-наноструктуры
- 4. Разветвленные наноструктуры, полученные модификацией ДНК
- 5. Молекулярные устройства на основе ДНК и обработка информации
- ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ
- 1. Синтез реагентов для модификации олигонуклеотидов
- 2. Отработка реакции [3+2]-диполярного циклоприсоединения на модельном азиде
- 3. Синтез флуоресцентно-меченых олигонуклеотидов по реакции [3+2]-диполярного циклоприсоединения
- 4. Синтез конъюгатов бисимида перилен-3,4,9,10-тетракарбоновой кислоты
- 5. Синтез олигонуклеотидных триконъюгатов и сборка наноструктур на их основе
- ЭКСПЕРИМЕНТАЛЬНАЯ ЧАСТ
- ВЫВОДЫ
- БЛАГОДАРНОСТИ
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