Π‘ΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠ΅ΡΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ² Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ
ΠΠ°ΠΎΠ±ΠΎΡΠΎΡ, ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ U3 ΠΎΠ±Π»Π°ΡΡΡ LTR ΠΈΠ· Π»ΠΎΠΊΡΡΠ° ΠΠ‘52 374 Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π² ΡΠ΅ΠΌΠΈΠ½ΠΎΠΌΠ΅ ΠΈ ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠ΅, Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ U5 ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠΉ ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡ ΡΡΠΎΠ³ΠΎ LTR Π½Π΅ Π΄Π΅ΡΠ΅ΠΊΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π½ΠΈ Π² ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½Π΅ΠΉ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π°Π½Π°Π»ΠΈΠ·Π° ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ LTR Π½Π° ΠΏΠ°Π½Π΅Π»ΠΈ ΠΊΠΠΠ ΠΈΠ· ΡΠ΅ΡΡΠΈ ΡΠΊΠ°Π½Π΅ΠΉ ΠΈ ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ½ΠΈΠΉ ΡΡΠΌΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π² ΡΠ°Π±Π»ΠΈΡΠ΅ 2.1. ΠΠ· ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ … Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠΠΠΠ 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- ΠΠ»Π°ΡΡΡ ΡΠ΅ΡΡΠΎΡΡΠ°Π½ΡΠΏΠΎΠ·ΠΎΠ½ΠΎΠ²
- 1. LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ
- 1. 1. Π‘ΡΡΡΠΊΡΡΡΠ° ΠΈ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ L1 ΡΠ»Π΅ΠΌΠ΅Π½ΡΠΎΠ²
- 1. 2. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ L1 ΠΏΠΎ Π³Π΅Π½ΠΎΠΌΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 1. 3. Π€ΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ L
- 1. LINE ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ
- 2. ΠΠ½Π΄ΠΎΠ³Π΅Π½Π½ΡΠ΅ ΡΠ΅ΡΡΠΎΠ²ΠΈΡΡΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 2. 1. Π‘ΡΡΠΎΠ΅Π½ΠΈΠ΅ HERV
- 2. 2. ΠΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ
- 2. 3. ΠΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Π½ΠΎΡΡΡ ΠΈ Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ HERV ΠΈ LTR Π² Π³Π΅Π½ΠΎΠΌΠ΅
- 2. 4. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ HERV ΠΈ LTR ΠΏΠΎ Ρ ΡΠΎΠΌΠΎΡΠΎΠΌΠ°ΠΌ
- 2. 5. ΠΠΊΡΠΈΠ²Π½ΠΎΡΡΡ HERV ΠΈ ΠΎΠ΄ΠΈΠ½ΠΎΡΠ½ΡΡ LTR
- 2. 6. Π€Π°ΠΊΡΠΎΡΡ, Π²Π»ΠΈΡΡΡΠΈΠ΅ Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ HERV
- 2. 7. ΠΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ΅ ΡΡΠ°ΡΡΠΈΠ΅ ERV Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΏΠ°ΡΠΎΡΠΈΠ·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠ°Ρ
- 2. 1. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR ΡΠ΅ΠΌΠ΅ΠΉΡΡΠ²Π° HERV-K
- 2. 2. ΠΠ½Π°Π»ΠΈΠ· ΡΡΠ°Π½ΡΠΊΡΠΈΠΏΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ LTR HERV-K Π² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΡΡ ΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΡΠΊΠ°Π½ΡΡ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 2. 3. ΠΠΎΠΈΡΠΊ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΡ Π΄Π»Ρ ΠΎΠΏΡΡ ΠΎΠ»Π΅ΠΉ ΠΈΠ½ΡΠ΅Π³ΡΠ°ΡΠΈΠΈ LTR HERV-K ΠΈ LINE
- 3. 1. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ
- 3. 1. 1. Π€Π΅ΡΠΌΠ΅Π½ΡΡ ΠΈ ΡΠ΅Π°ΠΊΡΠΈΠ²Ρ
- 3. 1. 2. ΠΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½ΠΈΠ΅
- 3. 1. 3. Π Π°ΡΡ ΠΎΠ΄Π½ΡΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ
- 3. 1. 4. ΠΡΡΠ΅ΡΠ½ΡΠ΅ ΠΈ Π΄ΡΡΠ³ΠΈΠ΅ ΡΠ°ΡΡΠ²ΠΎΡΡ
- 3. 1. 5. ΠΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΡΠ΅Π΄Ρ
- 3. 1. 6. Π’ΠΊΠ°Π½ΠΈ
- 3. 1. 7. ΠΡΠ°ΠΉΠΌΠ΅ΡΡ, ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ²
- 3. 2. ΠΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
- 3. 2. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π ΠΠ ΠΈ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ
- 3. 2. 2. ΠΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΠΠΠ°Π·ΠΎΠΉ
- 3. 2. 3. Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΠ΅ΡΠ²ΠΎΠΉ ΡΠ΅ΠΏΠΈ ΠΊΠΠΠ
- 3. 2. 4. PCR
- 3. 2. 5. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΡΡΡΡΠΊΡΡΡΡ ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ² ΠΠΠ ΠΈ PCR ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠΎΠ²
- 3. 2. 6. ΠΠΎΠ΄Π±ΠΎΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ² Π΄Π»Ρ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ
- 3. 2. 7. ΠΠΎΠΈΡΠΊ Π³ΠΎΠΌΠΎΠ»ΠΎΠ³ΠΈΠΉ, ΠΊΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π² Π³Π΅Π½ΠΎΠΌΠ΅ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°
- 3. 2. 8. ΠΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠΉ Π΄ΠΈΡΠΏΠ»Π΅ΠΉ SDDIR (Selective Differential Display of RNAs containing Interspersed Repeats)
- 3. 2. 9. ΠΠΎΠ΄ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠΉ TGDA (Targeted Genomic Difference Analysis)
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- ΠΠ²ΡΠΎΡ Π²ΡΡΠ°ΠΆΠ°Π΅Ρ Π³Π»ΡΠ±ΠΎΠΊΡΡ ΠΏΡΠΈΠ·Π½Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΈ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ½ΠΎΡΡΡ Π·Π° ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΡΠ²ΠΎΡΡΠ΅ΡΠΊΠΎΠΉ Π°ΡΠΌΠΎΡΡΠ΅ΡΡ, Π·Π° ΠΏΠΎΠΌΠΎΡΡ, ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΡ ΠΈ Π΄ΠΎΠ±ΡΠΎΠΆΠ΅Π»Π°ΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅ΠΌ ΡΠΎΡΡΡΠ΄Π½ΠΈΠΊΠ°ΠΌ ΠΈ Π°ΡΠΏΠΈΡΠ°Π½ΡΠ°ΠΌ ΠΠ°Π±ΠΎΡΠ°ΡΠΎΡΠΈΠΈ Π‘ΡΡΡΠΊΡΡΡΡ ΠΈ Π€ΡΠ½ΠΊΡΠΈΠΉ ΠΠ΅Π½ΠΎΠ² Π§Π΅Π»ΠΎΠ²Π΅ΠΊΠ° ΠΠΠ₯ Π ΠΠ.
- ΠΠ²ΡΠΎΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ΅Π½ Π. Π. ΠΠΎΡΠ°ΠΏΠΎΠ²Ρ ΠΈ Π. Π. Π‘ΠΊΠ°ΠΏΡΠΎΠ²ΠΎΠΉ Π·Π° ΡΠΎΡΡΡΠ΄Π½ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΈ ΡΠΈΠ½ΡΠ΅Π· ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΡΡ ΠΏΡΠ°ΠΉΠΌΠ΅ΡΠΎΠ², Π±Π΅Π· ΠΊΠΎΡΠΎΡΡΡ Π΄Π°Π½Π½Π°Ρ ΡΠ°Π±ΠΎΡΠ° Π±ΡΠ»Π° Π±Ρ Π½Π΅Π²ΠΎΠ·ΠΌΠΎΠΆΠ½Π°.
- ΠΠ²ΡΠΎΡ Π±Π»Π°Π³ΠΎΠ΄Π°ΡΠ΅Π½ Π‘Π΅Π½ΡΡΠ΅ ΠΠ°ΡΠ°Π»ΡΠ΅ ΠΠΎΡΠΈΡΠΎΠ²Π½Π΅ ΠΈ ΠΠ»Π΅ΠΉΠΌΠ°Π½ ΠΠ½Π½Π΅ Π·Π° ΡΠΎΡΡΡΠ΄Π½ΠΈΡΠ΅ΡΡΠ²ΠΎ ΠΈ ΠΏΡΠ΅Π΄ΠΎΡΡΠ°Π²Π»Π΅Π½Π½ΡΠ΅ Π΄Π»Ρ ΡΠ°Π±ΠΎΡΡ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ.