Получение рекомбинантных штаммов Aspregillus awamori — продуцентов ферментов для эффективной конверсии полисахаридов зернового сырья при производстве спирта
Диссертация
На основе новых рекомбинантных штаммов A. awamori 6804, А. awamori Т-14, A. awamori Т-29, A. awamori Xyl-15 и A. awamori EGI-73 наработаны опытные образцы концентрированных ферментных препаратов и проведены сравнительные исследования их гидролитической способности по отношению к полисахаридам зернового сырья. Установлено, что концентрация глюкозы при использовании ферментных препаратов… Читать ещё >
Содержание
- Список сокращений
- 1. Литературный обзор
- 1. 1. Технология производства пищевого спирта
- 1. 2. Ферменты, участвующие в гидролизе зернового сырья при производстве спирта
- 1. 3. Продуценты глюкоамилаз
- 1. 4. Методы получения штаммов-продуцентов промышленных ферментов
- 2. Материалы и методы исследования
- 2. 1. Штаммы грибов — продуцентов глюкоамилазы
- 2. 2. НГ-мутагенез и выделение niaD" мутантов как реципиентов для плазмидной трансформации
- 2. 3. Селекционные и ферментационные среды
- 2. 4. Получение штаммов-реципиентов A. awamor
- 2. 5. Плазмидные конструкции
- 2. 6. Получение протопластов, трансформация и селекция
- 2. 7. Условия облучения
- 2. 8. Тесты на стабильность продукции глюкоамилазы
- 2. 9. Определение активности ферментов
- 2. 10. Анализ состава ферментных препаратов
- 2. 11. Вискозиметрический метод определения общей эндодеполимеразной активности ксиланаз
- 2. 12. Исследования по биокатализу некрахмальных полисахаридов зернового сусла комплексными ферментными препаратами, полученными на основе рекомбинантных штаммов Aspergillus awamor
- 2. 13. Структура теоретических и экспериментальных исследований
- 3. Результаты и их обсуждение
- 3. 1. Выделение стабильного варианта штамма A. awamor
- 3. 2. Получение высокоэффективных штаммов A. awamori — продуцентов глюкоамилазы методом плазмидной трансформации
- 3. 3. Получение высокоэффективных штаммов A. awamori — продуцентов ферментов, гидролизующих НПС и фитиевую кислоту методом плазмидной трансформации
- 3. 4. Влияние индуктора на продуктивность рекомбинантных штаммов А. awamor
- 3. 5. Поддержание коллекции рекомбинатных штаммов A. awamor
- 3. 6. Исследование биоконверсии некрахмальных полисахаридов зернового сусла комплексными ферментными препаратами, полученными на основе высокоэффективных рекомбинантных штаммов Aspergillus awamor
- 3. 7. Масштабирование процесса культивирования рекомбинантных штаммов Aspergillus awamori в промышленных условиях
- 3. 8. Испытания ферментного препарата глюкоамилазы в спиртовом производстве
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- Я хотела бы выразить глубокую благодарность д.т.н., проф. Римаревой Любови Вячеславовне за руководство при выполнении и написании диссертационной работы.
- Выражаю глубокую и искреннюю признательность д.х.н., проф. Синицыну Аркадию Пантелеймоновичу, к.т.н. Цуриковой Нине Васильевне и к.х.н. Рожковой Александре Михайловне за помощь и руководство в выполнении диссертационной работы.