Регуляция экспрессии генов гуанилспецифичных рибонуклеаз Bacillus intermedius и Bacillus pumilus
Диссертация
Научная новизна работы. К началу настоящей работы публикации по биосинтезу рибонуклеаз бацилл были посвящены изучению регуляции синтеза ферментов факторами внешней среды. Новизна данной работы заключается в качественно новом уровне изучения регуляторных механизмов биосинтеза бациллярных рибонуклеаз. Впервые регуляция синтеза рибонуклеаз B. intermedius и B. pumilus экзогенным фосфатом была… Читать ещё >
Содержание
- ОБЗОР ЛИТЕРАТУРЫ
- 1. Сравнительная характеристика внеклеточных гуанилспецифичныхрибонуклеаз B. intermedius, B. pumilus, B. amyloliquefaciens
- 1. 1. Физико-химические свойства и структура белков
- 1. 2. Биосинтез рибонуклеаз. Клонирование генов
- 2. Механизмы фосфатной регуляции у бактерий
- 2. 1. РНО регулон у Escherichia col
- 2. 2. РНО регулон у Bacillus subtilis
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- Bacillus amyloliquefaciens >giI 493 894|pdbI1BRN|M
- A Bacillus amyloliquefaciens >giI 493 903|pdb11BSB|B
- A Bacillus amyloliquefaciens >gi|493 906|pdbI1BSCIB
- A Bacillus amyloliquefaciens >giI 493 909|pdbI1BSD|B
- A Bacillus amyloliquefaciens >giI 493 900 IpdbI1BSA|B
- A Bacillus amyloliquefaciens >giI 442 652|pdbI1BAN|B
- A Chain A, Barnase Mutant With lie 88 Replaced By
- A Bacillus amyloliquefaciens >giI 442 655|pdbI1BAO|B
- A Bacillus amyloliquefaciens >giI 576 012|pdb11BNS|B
- A Chain A, Barnase Mutant With Leu 14 Replaced By
- A Chain A, Barnase Mutant With He 76 Replaced By
- A Chain A, Barnase Mutant With lie 96 Replaced By
- A Chain A, Deletion Of A Buried Salt Bridge In Bar
- A Chain A, Structural Response To Mutation At A Pr
- A Chain A, Deletion Of A Buried Salt-Bridge In Bar
- A Bacillus amyloliquefaciens >gi|576 022|pdbI1BRG|B ribonuclease T2 (EC 3.1.27.1) Bacillus circul
- A Chain A, Barnase A43cS80C DISULFIDE MUTANT >gi|1
- A Bacillus amyloliquefaciens >gi|229 427|prfI I 72 194
- A Chain A, Structural Response To Mutation At A Pr
- A Chain A, Barnase T70cS92C DISULFIDE MUTANT >gi|1
- A Chain A, Deletion Of A Buried Salt Bridge In Bar
- Score = 327 bits (829), Expect = 6e-891.entities = 162/162 (100%), Positives = 162/162 (100%)1. Frame = +3
- Query: 177 MKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVHTLAVINTFD 356
- MKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVHTLAVINTFD Sbjct: 1 MKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVHTLAVINTFD 60
- Query: 357 GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG 536
- GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG Sbjct: 61 GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG 120
- Query: 537 RTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 318 bits (807), Expect = 2e-861.entities = 158/162 (97%), Positives = 160/162 (98%)1. Frame = +3
- Query: 177 MKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVHTLAVINTFD 356
- MKKISSVFTMFALIAAILFSGFIPQQAYAET LT TATN+TA+IQLTSDVHTLAVINTFD Sbjct: 1 MKKISSVFTMFALIAAILFSGFIPQQAYAETTLTPTATNKTASIQLTSDVHTLAVINTFD 60
- Query: 357 GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG 536
- GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG Sbjct: 61 GVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGRLPSASG 120
- Query: 537 RTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- RTWREADINYVSG FRNADRLVYS SDWLIYKTTDHYATFTRIR Sbjct: 121 RTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 162pdb|1BUJ| Structure Of Binase In Solution Length = 109
- Score = 225 bits (567), Expect = 3e-581.entities = 108/108 (100%), Positives = 108/108 (100%)1. Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 3bjct: 2 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 612uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 224 bits (566), Expect = 4e-581.entities = 108/109 (99%), Positives = 108/109 (99%)1. Frame = +3
- Query: 336 AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG 515
- AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG Sbjct: 1 AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG 60
- Query: 516 RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATF RIR Sbjct: 61 RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFARIR 109pdbI2RBI1A Chain A, Structure Of Binase Mutant His 101 Asngi|1 942 121|pdb|2RBI|B Chain B, Structure Of Binase Mutant His 101 Asn Length = 109
- Score = 224 bits (564), Expect = 7e-581.entities = 108/109 (99%), Positives = 109/109 (99%)1. Frame = +3
- Query: 336 AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG 515
- AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG Sbjct: 1 AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG 60
- Query: 516 RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTD+YATFTRIR Sbjct: 61 RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDNYATFTRIR 109pir||NRBSI ribonuclease (EC 3.1.-.-) Bacillus «intermedius» Length = 109
- Score = 217 bits (546), Expect = 9e-561.entities = 104/109 (95%), Positives = 107/109 (97%)1. Frame = +3
- Query: 336 AVINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREG 515
- AVINTFDGVADYLIRYKRLP++YITKSQASALGWVASKG+LAEVAPGKSIGGDVFSNREG Sbjct: 1 AVINTFDGVADYLIRYKRLPNDYITKSQASALGWVASKGDLAEVAPGKSIGGDVFSNREG 60
- Query: 516 RLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 202 bits (508), Expect = 3e-51 Identities = 91/108 (84%), Positives = 102/108 (94%) Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL+ Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLLTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Query: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662 LP+ SGRTWREADINY SGFRN+DR++YSSDWLIYKTTDHY TFT+IR
- Sbjct: 63 LPAKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYKTFTKIR 110pdb|lBSE|a Bacillus amyloliquefaciens >gi|4 93 912|pdb|1BSE|B Bacillus amyloliquefaciens >giI 493 913 IpdbI1BSE|C Bacillus amyloliquefaciens Length = 110
- Score = 201 bits (505), Expect = 6e-511.entities = 92/108 (85%), Positives = 100/108 (92%)1. Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 200 bits (504), Expect = 8e-511.entities = 94/112 (83%), Positives = 103/112 (91%), Gaps = 1/112 (0%)1. Frame = +3
- Query: 327 HTLA-VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFS 503
- HT A VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FS Sbjct: 37 HTEAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFS 96
- Query: 504 NREGRLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 200 bits (504), Expect = 8e-511.entities = 94/112 (83%), Positives = 103/112 (91%), Gaps = 1/112 (0%)1. Frame = +3
- Query: 327 HTLA-VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFS 503
- HT A VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FS Sbjct: 45 HTEAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFS 104
- Query: 504 NREGRLPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- NREG+LP SGRTWREADINY SGFRN+DR++YSSDWLIYKTTDHY TFT+IR Sbjct: 105 NREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR 157giI 1 439 559 (U46664) barnase synthetic construct. Length = 111
- Score = 199 bits (502), Expect = le-501.entities = 91/108 (84%), Positives = 100/108 (92%)1. Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 4 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 63
- Query: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Structure At Ph 6.0 >gi11127179|pdb|1BNI|B Chain B,
- Barnase Wildtype Structure At Ph 6.0gi11127180 Ipdb11BNI|C Chain C, Barnase Wildtype
- Structure At Ph 6.0 >giI 112 7282Ipdbl1BNRI Barnasegi|32 122 68|pdb|1A2P|A Chain A, Barnase Wildtype
- Structure At 1.5 Angstroms Resolutiongi.32 122 69|pdb|1A2P|B Chain B, Barnase Wildtype
- Structure At 1.5 Angstroms ResolutiongiI 3 212 270 Ipdb11A2P|C Chain C, Barnase Wildtype
- Structure At 1.5 Angstroms ResolutiongiI 3 745 778 Ipdb11BV0|A Chain A, Structural Response To
- Mutation At A Protein-Protein InterfacegiI 374 5779Ipdbl1BV0IB Chain B, Structural Response To
- Mutation At A Protein-Protein InterfacegiI 374 5780Ipdbl1BV0IC Chain C, Structural Response To
- Mutation At A Protein-Protein InterfacegiI 413 9697Ipdbl1B2X|A Chain A, Barnase Wildtype
- Structure At Ph 7.5 From A Cryocooled Crystal At 100kgiI 413 9698Ipdbl1B2XIB Chain B, Barnase Wildtype
- Structure At Ph 7.5 From A Cryocooled Crystal At 100kgiI 413 9699Ipdbl1B2XIC Chain C, Barnase Wildtype
- Structure At Ph 7.5 From A Cryocooled Crystal At 100kgi|4 139 778|pdb|1B27|A Chain A, Structural Response To
- Mutation At A Protein-Protein InterfacegiI 413 9779Ipdbl1B27IB Chain B, Structural Response To
- Mutation At A Protein-Protein InterfacegiI 4 139 780 Ipdb|1B27|C Chain C, Structural Response To
- Mutation At A Protein-Protein Interfacegi|4 388 896|pdb|1YVS| Trimeric Domain Swapped BarnasegiI 39 310 IembICAA31365| (X12871) barnase Bacillusamyloliquefaciens.1.ngth = 110
- Score = 199 bits (502), Expect = le-50 Identities = 91/108 (84%), Positives = 100/108 (92%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 199 bits (501), Expect = 2e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62iuery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 199 bits (501), Expect = 2e-50 Identities = 91/108 (84%), Positives = 100/108 (92%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 199 bits (501), Expect = 2e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 199 bits (501), Expect = 2e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKS+GGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSVGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (499), Expect = 3e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (499), Expect = 3e-50 Identities = 91/108 (84%), Positives = 99/108 (91%) Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Query: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (498), Expect = 4e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- ViNTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Query: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (497), Expect = 5e-50 Identities = 90/108 (83%), Positives 99/108 (91%) Frame = +33uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYI KS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYIAKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (497), Expect = 5e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADY Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Bbjct: 3 VINTFDGVADYAQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (497), Expect = 5e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (497), Expect = 5e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +3
- Query: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Query: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 198 bits (497), Expect = 5e-50 Identities = 90/108 (83%), Positives = 100/108 (92%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDN YI TKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 197 bits (496), Expect = 7e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYIT S+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITASEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 197 bits (496), Expect = 7e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjet: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 197 bits (496), Expect = 7e-50 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINT DGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ 3bjct: 1 VINTLDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 602uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 196 bits (493), Expect = 2e-49 Identities = 89/108 (82%), Positives = 100/108 (92%) Frame = +32uery: 339 VINT FDGVADYLIRYKRL PDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- Score = 196 bits (493), Expect = 2e-49 Identities = 89/108 (82%), Positives = 98/108 (90%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNL +VAPGKSIGGD+FSNREG+ sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLCDVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- SGRTWREADINY GFRN+DR++YSSDWLIYKTTDHY TFT+IR Sbjct: 63 LPGKSGRTWREADINYTCGFRNSDRILYSSDWLIYKTTDHYQTFTKIR 110pdb|1RNB|A Bacillus amyloliquefaciens >gi|229 427|prf||72 1946A RNase Bacillus amyloliquefaciens. Length = 110
- Score = 196 bits (492), Expect = 2e-49 Identities = 89/108 (82%), Positives = 100/108 (92%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LP++YITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPNDYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 196 bits (492), Expect = 2e-49 Identities = 90/108 (83%), Positives = 99/108 (91%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 195 bits (491), Expect = 3e-49 Identities = 89/108 (82%), Positives = 98/108 (90%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 622uery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 195 bits (491), Expect = 3e-491.entities = 89/108 (82%), Positives = 99/108 (91%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Juery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- Score = 195 bits (490), Expect = 3e-49 Identities = 90/108 (83%), Positives = 98/108 (90%) Frame = +32uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWVASKGNLAEVAPGKSIGGDVFSNREGR 518
- VINTFDGVADYL Y +LPDNYITKS+A ALGWVASKGNLA+VAPGKSIGGD+FSNREG+ Sbjct: 3 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGK 62
- Juery: 519 LPSASGRTWREADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRIR 662
- SGRTWREADINY SGFRN DR++YSSDWLIYKTTD Y TFT+IR Sbjct: 63 LPGKSGRTWREADINYTSGFRNCDRILYSSDWLIYKTTDCYQTFTKIR 110bbsI 139 914 ribonuclease Bci I- RNAase Bci I Length = 36
- Score =64.4 bits (154), Expect = 9e-10 Identities = 28/34 (82%), Positives = 31/34 (90%) Frame = +3uery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWV 440
- VINTFDGVADYL+ Y +LPDNYITKS+A ALGWV Sbjct: 3 VINTFDGVADYLLTYHKLPDNYITKSEAQALGWV 3 6embICAA07009I (AJ006407) barnase Bacillus intermedius. Length = 38
- Score = 62.5 bits (149), Expect = 3e-09 Identities = 28/34 (82%), Positives = 30/34 (87%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQASALGWV 440
- VINTFDGVADYL Y +LPDNYITKS+A ALGWV Sbjct: 5 VINTFDGVADYLQTYHKLPDNYITKSEAQALGWV 38bbs|139 916 ribonuclease Bci II, RNAase Bci II {N-terminal} Bacillus, BCF 247, Peptide Partial, 43 aa. Length = 43
- Score = 57.0 bits (135), Expect = le-071.entities = 28/35 (80%), Positives = 31/35 (88%), Gaps = 1/35 (2%) Frame = +3uery: 327 HTLA-VINTFDGVADYLIRYKRLPDNYITKSQASAL 431
- HT A VINTFDGVADYL+ Y +LPDNYITKS+A AL Sbjct: 8 HTEAQVINTFDGVADYLLTYHKLPDNYITKSEAQAL 43bbs|125 249 extracellular alkaline ribonuclease {N-terminal} Bacillus thuringiensis, var. subtoxicus, Peptide Partial, 25 aa. Length =25
- Score = 55.4 bits (131), Expect = 4e-07 Identities = 25/25 (100%), Positives = 25/25 (100%) Frame = +3
- Juery: 336 AVINTFDGVADYLIRYKRLPDNYIT 410
- AVINTFDGVADYLIRYKRLPDNYIT Sbjct: 1 AVINTFDGVADYLIRYKRLPDNYIT 25bbsI 148 781 alkaline ribonuclease, alkaline RNase {N-terminal} {EC 3.1.4.23} Bacillus subtilis, Kolyma lowland isolate, BCF 256, Peptide Partial, 24 aa. Length = 24
- Score = 53.5 bits (126), Expect = 2e-06 Identities = 24/24 (100%), Positives = 24/24 (100%) Frame = +32uery: 336 AVINTFDGVADYLIRYKRLPDNYI 407
- AVINTFDGVADYLIRYKRLPDNYI 3bjct: 1 AVINTFDGVADYLIRYKRLPDNYI 24bbs|179 881 Bpo 11=12.608 kda ribonuclease isoform II {N-terminal} Bacillus polymyxa, 514, Peptide Partial, 32 aa. Length = 32
- Score = 51.9 bits (122), Expect = 5e-06 Identities = 21/29 (72%), Positives = 28/29 (96%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYITKSQAS 425
- VINTF+GVADY+++Y RLPDN+ITK++AS 3bjct: 4 VINTFEGVADYIVKYGRLPDNFITKAEAS 32bbs|148 782 alkaline ribonuclease, alkaline RNase {N-terminal} {EC 3.1.4.23} Bacillus subtilis, Kolyma lowland isolate, BCF 247, Peptide Partial, 25 aa. Length = 25
- Score = 43.4 bits (100), Expect = 0.002 Identities = 19/23 (82%), Positives = 20/23 (86%) Frame = +3
- Juery: 339 VINTFDGVADYLIRYKRLPDNYI 407
- VINTFDGVADYL Y +LPDNYI Jbjct: 3 VINTFDGVADYLQTYHKLPDNYI 25bbs|179 882 Bpo 111=13.18 kda ribonuclease isoform III {N-terminal} Bacillus polymyxa, 514, Peptide Partial, 31 aa. Length = 31
- Score = 42.6 bits (98), Expect = 0.003 Identities = 17/27 (62%), Positives = 23/27 (84%) Frame = +32uery: 324 VHTLAVINTFDGVADYLIRYKRLPDNY 404
- V + VINTF+GVADY+++Y RLPDN+ >bjct: 5 VQSNEVINTFEGVADYIVKYGRLPDNF 31spIP30289|RNS3STRAU GOANYL-SPECIFIC RIBONUCLEASE SA3 PRECURSOR >gi|98 876|pir||JC1287 ribonuclease Sa (EC 3.1.27.-) precursor Streptomyces aureofaciens (strain CCM3239) Length = 141
- Score =39.1 bits (89), Expect = 0.0361.entities = 25/57 (43%), Positives = 31/57 (53%), Gaps = 2/57 (3%) Frame = +3
- Juery: 489 GDVFSNREGRLPSASGRTWREAD-INYVSGFRNADRLVYSSDWLI-YKTTDHYATFTRI 659
- Score = 34.8 bits (78), Expect = 0.711.entities = 21/54 (38%), Positives = 26/54 (47%), Gaps = 1/54 (1%) Frame = +3uery: 489 GDVFSNREGRLPSASGRTWREADINYVSG-FRNADRLVYSSDWLIYKTTDHYATF 650
- G VF NREG LP + +E+ SGRARV +T DHY +Fibjct: 41 GTVFENREGILPDCAEGYYHEYTVKTPSGDDRGARRFVVGDGGEYFYTEDHYESF 95gi11079693 (U39202) ribonuclease St synthetic construct. Length = 102
- Score =34.8 bits (78), Expect =0.711.entities = 21/54 (38%), Positives = 26/54 (47%), Gaps = 1/54 (1%) Frame = +3uery: 489 GDVFSNREGRLPSASGRTWREADINYVSG-FRNADRLVYSSDWLIYKTTDHYATF 650
- G VF NREG LP + +E + SG RARV +T DHY +Fbjct: 42 GTVFENREGILPDCAEGYYHEYTVKTPSGDDRGARRFVVGDGGEYFYTEDHYESF 96gi|3 242 716 (AC003040) hypothetical protein Arabidopsis thaliana. Length = 555
- Score = 33.2 bits (74), Expect = 2.1 Identities = 27/92 (29%), Positives = 43/92 (46%) Frame = +3luery: 153 DFLNRRMKMKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVHT 332
- D +N +K+ +1 + F +AI I + + T+ A + L+ D Ibjct: 188 DMVNMCLKLLRIQTGFVPLVAGSAIAVCAGIIKDGFQLARFTEGAED----FFLSLDCFQ 243luery: 333 LAVINTFDGVADYLIRYKRLPDNYITKSQASA 428
- A + + VA YLI+ KR P + T SQASA Ibjct: 244 IAALG-YKSVAHYLIQTKRAPTDDTTPSQASA 274gi 1 153 424 (M88615) ribonuclease Streptomyces aureofaciens. Length = 163
- Score =32.8 bits (73), Expect =2.71.entities = 22/57 (38%), Positives = 26/57 (45%), Gaps = 1/57 (1%) Frame = +3luery: 489 GDVFSNREGRLPSASGRTWRE-ADINYVSGFRNADRLVYSSDWLIYKTTDHYATFTRI 659
- G VF NRE RLP + E + S R R+V Y + DHYATF I1. jct: 102 GVVFENRESRLPKKGNGYYHEFTVVTPGSNDRGTRRVVTGGYGEQYWSPDHYATFQEI 159gi13243108 (AF034976) T7-like RNA polymerase Pilayella littoralis. Length = 824
- Score = 31.3. bits (69), Expect = 8.11.entities = 18/67 (26%), Positives = 35/67 (51%), Gaps = 1/67 (1%) Frame = +1luery: 4 87 VEMFSLTG-RDVFLQQAAEHGVRQISTTSLASEMLTASCIQVTGSFTKQQTIMQLSHVFD 663
- FSL +D+F+ + + T+ L +TAS +Q+ G T+ ++++ VFD bjct: 478 IDDFSLHALKDIFINEGGQ-------TSQLIGLDVTASGLQIMGLITRCTKALEMTQVFD 530luery: 664 NQSKNSPI 687 NS +bjct: 531 QNETNSAV 538
- Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR
- I I I I I I I I I I I I I I I Jbjct: 7571 cttccgaaatgctgac 7586emb1X92868|BS233DEG B. subtilis 23.9kb fragment from map position 233 degrees on the chromosome
- Score =32.2 bits (16), Expect =1.4 Identities = 16/16 (100%) Strand = Plus / Plusuery: 575 cttccgaaatgctgac 590
- I I II I I I I I I I I I I I I Jbjct: 21 452 cttccgaaatgctgac 21467emb|Z99106IBSUB0003 Bacillus subtilis complete genome (section 3 of 21): from 402 751 to 6 118 501. ngth = 209 100
- Score =32.2 bits (16), Expect =1.4 Identities = 16/16 (100%) Strand = Plus / Minusuery: 212 tctgatcgctgctatt 227
- I I I II I I I I I I I I I I! bjct: 3975 tctgatcgctgctatt 3960emb|Z99123|BSUB0020 Bacillus subtilis complete genome (section 20 of 21): from 3 798 401 t 40 105 501. ngth = 212 150
- Score =32.2 bits (16), Expect = 1.4 Identities = 19/20 (95%) Strand = Plus / Minus2uery: 674 aaaacagccccatttgacgt 693
- I I I I I II I I I I I I I I I I I >bjct: 64 867 aaaaccgccccatttgacgt 64 848
- Database: Non-redundant GenBank+EMBL+DDBJ+PDB sequences
- VINTFDGVADYL Y +LPDNYI >b jct: 3 VINTFDGVADYLQTYHKLPDNYI 25pir| |A26874 cellulase (EC 3.2.1.4) precursor Bacillus subtilis (strain DLG) >gi1143008 (M16185) endo-beta-1,4-glucanase Bacillus subtilis. Length = 508
- Score = 29.7 bits (65), Expect = 0.79 Identities = 18/55 (32%), Positives = 26/55 (46%) Frame = +3uery: 165 RRMKMKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVH 329
- R MK+ S+F LIA + G+PAA TAN +1+ T V+ 3b j ct: 6 KRSDMKRSISIFITCLLIAVLTMGGLLPSPASAAGTKTPVAKNGQLSIKGTQLVN 60sp|P07983|GUN1BACSU ENDOGLUCANASE PRECURSOR (ENDO-1,4-BETA-GLUCANASE) (CELLULASE) Length = 4 99
- Score = 27,8 bits (60), Expect =3.1 Identities = 17/51 (33%), Positives = 24/51 (46%) Frame = +3
- Juery: 177 MKKISSVFTMFALIAAILFSGFIPQQAYAETPLTQTATNETATIQLTSDVH 329
- MK+ S+F LIA + G+PAA TAN +1+ T V+ 3bj ct: 1 MKRSISIFITCLLIAVLTMGGLLPSPASAAGTKTPVAKNGQLSIKGTQLVN 51
- Database: Non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PIR
- Posted date: Sep 2, 1999 2:50 PM Number of letters in database: 124,895,471 Number of sequences in database: 407,989jambda 0.3181. K H0135 0.4011. Sappedjambda K H0270 0.0470 0.2304atrix: BLOSUM62
- Sap Penalties: Existence: 11, Extension: 1
- Cumber of Hits to DB: 1 804 369
- Cumber of Sequences: 407 989
- Cumber of extensions: 33457slumber of successful extensions: 89
- Cumber of sequences better than 10.0: 8
- Cumber of HSP’s better than 10.0 without gapping: 4
- Cumber of HSP’s successfully gapped in prelim test: 0
- Cumber of HSP’s that attempted gapping in prelim test: 85