Регуляция нитрогеназной активности и фотообразования водорода у пурпурных несерных бактерий
Диссертация
Актуальность проблемы. В последние годы в мире резко возрос интерес к альтернативным источникам энергии. Это обусловлено, прежде всего, размерами экологических последствий использования традиционных энергетических ресурсов (угля, нефти и природного газа). Молекулярный водород является экологически чистым источником энергии, поскольку продуктом его сгорания является вода. В настоящее время… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- ЛИТЕРАТУРНЫЙ ОБЗОР
- Глава 1. Общая характеристика пурпурных несерных бактерий
- Глава 2. Азотфиксирующая система пурпурных бактерий
- 2. 1. Нитрогеназа: строение и свойства
- 2. 2. Гены, контролирующие процесс азотфиксации
- 2. 3. Регуляция нитрогеназной системы
- 2. 3. 1. Регуляция экспрессии нитрогеназы
- 2. 3. 2. Действие аммония на нитрогеназную активность in vivo
- 2. 3. 3. Молекулярные механизмы посттрансляционной регуляции нитрогеназы
- 3. 1. Факторы, влияющие на фотообразование Н2 у суспензионных культур
- 3. 1. 1. Свет
- 3. 1. 2. Доноры электронов и источники углерода
- 3. 1. 3. Источники азота
- 3. 1. 4. Температура, рН и другие факторы
- 3. 2. Иммобилизованные культуры. Особенности выделения водорода
- 4. 1. Объекты исследований
- 4. 2. Среды для культивирования
- 4. 3. Определение нитрогеназной активности клеток
- 4. 4. Определение характеристик эффекта выключения нитрогеназы аммонием
- 4. 5. Определение АДФ-рибозилирования Fe-белка нитрогеназы
- 4. 6. Определение концентраций различных соединений
- 4. 6. 1. Определение содержания лактата и пирувата
- 4. 6. 2. Определение аммония
- 4. 6. 3. Определение концентрации сухой биомассы, белка и бактериохлорофилла а
- 4. 6. 4. Определение содержания пиридиннуклеотидов
- 4. 6. 5. Измерение образования Н2 в ФБР
- 4. 7. Измерение интенсивности падающего света
- 4. 8. Активация поверхности стекла и иммобилизация фототрофных микроорганизмов
- 4. 9. Характеристика сточных вод
- 4. 10. Фотобиореакторы
- 4. 10. 1. ФБР для суспензионных культур
- 4. 10. 2. ФБР для изучения фотообразования водорода иммобилизованными культурами
- 5. 1. Влияние обеспеченности аммонием на нитрогеназную активность клеток Rba. capsulatus и Rba. sphaeroides и на эффект выключения нитрогеназы
- 5. 2. Влияние концентрации биомассы на нитрогеназную активность клеток Rba. capsulatus и на эффект выключения нитрогеназы
- 5. 3. Влияние рН на нитрогеназную активность и эффект выключения нитрогеназы у Rba. capsulatus
- 5. 4. Влияние азотного голодания и недостатка органического субстрата на нитрогеназную активность и эффект выключения нитрогеназы у Rba. capsulatus
- 6. 1. Связь эффекта выключения и АДФ-рибозилирования Fe-белка нитрогеназы
- 6. 1. 1. Наличие эффекта выключения и АДФ-рибозилирования у некоторых пурпурных несерных бактерий
- 6. 1. 2. Эффект выключения у Rba. capsulatus и ее мутантов с нарушенной системой модификации Fe-белка
- 6. 1. 3. Эффект выключения нитрогеназы и АДФ-рибозилирование у Rba. sphaeroides и ее производных с внедрёнными генами nifHDK и draT/G из Rba. capsulatus
- 6. 2. Эффект выключения нитрогеназной активности аммонием и степень восстановленности пиридиннуклеотидов
- 7. 1. Влияние концентрации аммония в подаваемой среде на выделение водорода хемостатной культурой Rba. capsulatus
- 7. 2. Влияние скорости протока на выделение водорода хемостатной культурой Rba. capsulatus
- 7. 3. Выявление факторов, снижающих скорость образования водорода хемостатной культурой Rba. capsulatus
- 7. 4. Фотообразование водорода хемостатной культурой amtB'-мутанта Rba. capsulatus, не обладающего эффектом выключения нитрогеназы аммонием
- 8. 1. Иммобилизация на стекле
- 8. 2. Получение водорода в ФБР с иммобилизованными на пористом стекле клетками пурпурных несерных бактерий
- 8. 3. Получение водорода в ФБР с иммобилизованными на пенополиуретане клетками пурпурных несерных бактерий
- 8. 4. Возможность использования сточных вод различными пурпурными несерными бактериями
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