Психрофильные морские светящиеся бактерии Aliivibrio logei: структура lux-оперона и регуляция типа «Quorum sensing»
Диссертация
Использование транскрипционных слияний индуцируемых промоторов (чувствительных к аутоиндукторам (АИ) первого типа) психрофильных бактерий, с генами термостабильных бактериальных люцифераз, в качестве репортерных, позволило создать высокочувствительные, специфически реагирующие на АИ первого типа биосенсоры. Данные биосенсоры в настоящее время применяются для работ по экологическому мониторингу… Читать ещё >
Содержание
- Список используемых сокращений. стр
- ВВЕДЕНИЕ.стр
- Глава 1. ОБЗОР ЛИТЕРАТУРЫ. стр
- 1. 1. Психрофильные светящиеся морские бактерии. Белки «холодового шока». стр
- 1. 2. Системы типа «Quorum Sensing».стр
- 1. 2. 1. LuxI/LuxR -зависимая регуляция биолюминесценции у морских бактерий. стр
- 1. 2. 2. Ацил-Ь-гомосерин лактоны (AHL) и их взаимодействие с LuxR-подобными белками. стр
- 1. 2. 3. Внутриклеточные факторы регуляции экспрессии генов, входящих в опероны систем QS. стр
- 2. 1. Бактериальные штаммы и плазмиды. стр
- 2. 2. Среды и условия культивирования. стр
- 2. 3. Генно-инженерные методы. стр
- 2. 3. 1. Препараты ферментов. стр
- 2. 3. 2. Химические реагенты. стр
- 2. 3. 3. Методы работы с бактериями. стр
- 2. 3. 4. Манипуляции с ДНК. стр
- 2. 3. 5. Полимеразная цепная реакция. стр
- 2. 3. 6. Определение нуклеотидной последовательности. стр
- 2. 3. 7. Мульти-генный анализ. стр
- 2. 3. 8. Измерение интенсивности биолюминесценции. стр
- 2. 4. Биохимическое тестирование. стр
- 2. 5. Измерение антибиотикограмм. стр
- 3. 1. Идентификация психрофильных морских светящихся бактерий, изолированных в акваториях Белого, Охотского и Берингова морей., стр
- 3. 1. 1. Определение рода по гену 16S рРНК и биохимические характеристики изолированных штаммов. стр
- 3. 1. 2. Мультигенный анализ. стр
- 3. 1. 3. Чувствительность к антибиотикам. стр
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