Изучение вариаций в организации и экспрессии оперонов рибосомных белков зубактерий

выводы.

1) Найдены существенные различия в организации генов рибосомных (р-) белков в геномах, а также в организации цистронов в оперонах р-белков ряда эубактерий: Е. coli, V. cholerae, S. Typhi, M. tuberculosis, S. aureus.

2) Идентифицированы 25 стабильных бактериальных коротких РНК (бкРНК), картированных на обоих тяжах оперонов р-белков (смысловых и антисмысловых), что расширяет возможности регуляции экспрессии.

3) Для лидерной области альфаи LlO-оперонов р-белков Е. coli, найдены смысловые бкРНК, соответствующие районам псевдоузла и мотива «kink-turn», соответственно, которые участвуют в регуляции трансляции.

4) Методом ПЦР в реальном времени (ПЦР РВ) найдена повышенная представленность концевого участка цистрона EF-G, что указывает на наличие активного внутреннего промотора sí-r-оперона Е. coli.

5) Повышенная представленность межцистрона S12-S7 .s/r-оперона Е. coli определяется его предполагаемым участием в регуляции трансляции по типу обратной связи белком S7.

6) Изменение паттерна представленности цистронов альфаи ?/г-оперонов мутантного штамма Е. coli рпр" указывает на участие деградосом в определении уровня представленности различных участков str мРНК.

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