Новые анаэробные термофильные прокариоты и их гидролитические ферменты
Диссертация
На сегодняшний день ферменты широко используются человеком в таких областях применения как производство продуктов питания и напитков, химическая промышленность, фармакология, медицина, целлюлозобумажная промышленность, и многих других (Copeland, 2000). Большинство ферментов, нашедших применение на данный момент, было обнаружено и выделено из организмов, обитающих при средних, нейтральных в нашем… Читать ещё >
Содержание
- ВВЕДЕНИЕ
- ОБЗОР ЛИТЕРАТУРЫ
- Глава 1. Термофильные микроорганизмы и их ферменты — термозимы
- 1. 1. Экстремофилы
- 1. 2. Термальные местообитания и термофилы
- 1. 3. Термозимы — ферменты термофильных микроорганизмов
- 1. 4. Механизмы, обуславливающие стабильность белков
- 1. 5. Термостабильные гидролазы
- 1. 5. 1. Амилазы
- 1. 5. 2. Целлюлазы
- 1. 5. 3. Ксиланазы
- 1. 5. 4. Хитиназы
- 1. 5. 5. Агаразы
- 1. 5. 6. Эстеразы и липазы
- Глава 2. Протеолитики и протеазы
- 2. 1. Природные белковые полимеры как субстраты протеаз
- 2. 2. Общие сведения. Классификация
- 2. 3. Термофильные протеолитики и их протеазы
- 2. 3. 1. Протеиназы термофильных бактерий
- 2. 3. 2. Протеиназы термофильных архей
- 3. 1. Отбор образцов и полевые работы
- 3. 2. Культивирование термофильных прокариот в лабораторных условиях
- 3. 3. Выделение чистых культур
- 3. 4. Микроскопия
- 3. 5. Оценка скорости роста
- 3. 6. Определение продуктов роста исследуемых штаммов
- 4. 1. Выделение ДНК
- 4. 2. Амплификация фрагментов генов, кодирующих 16S рРНК
- 4. 3. Детекция продуктов ПЦР
- 4. 4. Денатурирующий градиентный гель электрофорез (ДГГЭ)
- 4. 5. Секвенирование ПЦР-фрагментов и анализ нуклеотидных последовательностей
- 4. 6. Филогенетический анализ исследуемых последовательностей
- 5. 1. Приготовление образцов протеиназ
- 5. 2. ДСН-ПААГ электрофорез и зимограммы
- 5. 3. Гидролиз казеина
- 5. 4. Определение характеристик исследуемых кератиназ
- 5. 5. Гидролиз B-цепи инсулина исследуемыми кератиназами
- 5. 6. Очистка кератиназы из 'Caldanaerobacterproteolyticus' штамм
- 5. 7. Определение концентрации белка Результаты и обсуждение
- 6. 1. Фенотипическое разнообразие термофильных прокариот-гидролитиков в горячих источниках кальдеры Узон
- 6. 2. Филогенетическое разнообразие термофильных прокариот-гидролитиков в горячих источниках кальдеры Узон
- 6. 3. Протеолитическая активность в накопительных культурах
- 7. 1. Dictyoglomus sp. штаммы 1521−1 и
- 7. 2. 'Caldicellulosiruptor hydrothermalis' штамм 108 и 'Caldicellulosiruptor hydrothermalis' штамм
- 7. 3. 'Caldanaerobacterproteolyticus' штамм
- 7. 4. Thermoanaerobacter sp. штамм
- 7. 5. Thermoanaerobacterium aciditolerans штамм
- 7. 6. 'Desulfurococcus kamchatkensis' штамм 122In
- 8. 1. Целлюлазы из 'Caldicellulosiruptor hydrothermalis' штамм 108, 'Caldicellulosiruptor kronotskyensis' штамм 2002 и Dictyoglomus sp. штамм
- 8. 2. Протеиназа из 'Desulfurococcus kamchatkensis' штамм 122In 8.3 Рост штаммов 1523−1 и 1004 на белках, включаяя кератины, и продукция ими внеклеточных протеиназ
- 8. 4. Определение кератинолитической активности 'Caldanaerobacter proteolyticus' штамм 1523−1 и Thermoanaerobacter sp. штамм
- 8. 5. Специфичность кератиназ 1523−1 и
- 8. 6. Зависимость активности кератиназ 1523−1 и 1004 от Т и pH
- 8. 7. Физико-химические свойства кератиназ 1523−1 и
- 8. 8. Очистка кератиназы
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