Свойства, структура и функции факторов терминации трансляции eRF1 и eRF3
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Диссертация
ГТФазный цикл эуи прокариотических факторов терминации трансляции 2-го класса значительно отличаются. eRF3 не нуждается в классическом GEF, в отличие от своего прокариотического аналога RF3 (Zavialov et al., 2001), поскольку в eRF3 обмен нуклеотидов не связан с падением аффинности ГДФ к eRF3 в результате связывания eRFl. Картина совершенно иная, eRFl не нарушает способность eRF3 связывать ГДФ… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ 5 стр. ВВЕДНИЕ
- 1. Глава 1. ТЕРМИНАЦИЯ ТРАНСЛЯЦИИ (ОБЗОР ЛИТЕРАТУРЫ)
- Ы.Терминация трансляция опосредована белковыми факторами 1-го и 2-го классов
- 1. 2. Особенности структуры факторов терминации 2-го класса
- 1. 2. 1. Структурные особенности G домена ГТФаз
- 1. 2. 2. Первичная и третичная структуры RF3 и eRF
- 1. 3. ГТФазный цикл в рибосоме
- 1. 4. Функциональные свойства eRF
- 1. 4. 1. Роль eRF3 в терминации трансляции у эукариот
- 1. 4. 2. Нетерминационные функции eRF
- 1. 4. 3. Взаимодействие eRF3 и eRFl
- 1. 5. eRF3 не является функциональным аналогом RF
- 1. 6. Структурно-функциональные свойства eRFl
- 1. 2. Особенности структуры факторов терминации 2-го класса
- 2. Глава 2. МАТЕРИАЛЫ И МЕТОДЫ ИССЛЕДОВАНИЙ
- 2. 1. Штаммы бактерий, плазмиды, среды и реактивы
- 2. 2. Методы исследований
- 2. 2. 1. Выделение плазмидной ДНК из Е. col
- 2. 2. 2. Трансформация Е. coli плазмидной ДНК
- 2. 2. 3. Синтез и выделение белков
- 2. 2. 3. 1. Получение eRFl человека для исследования методом малоуглового рассеяния
- 2. 2. 3. 2. Получение еШ71 и мутантов еШ71 человека для исследования калориметрическими методами
- 2. 2. 3. 3. Получение еКБЗ человека
- 2. 2. 3. 4. Получение доменов еЮ7! человека
- 2. 2. 3. 4. 1. Получение ^ М и КМ доменов
- 2. 2. 3. 4. 2. Получение С и МС доменов
- 2. 2. 4. Электрофорез белков в денатурирующем 12% ПААГ
- 2. 2. 5. Измерение флуоресценции растворов белков
- 2. 2. 6. Измерения малоуглового рассеяния
- 2. 2. 7. Дифференциальная сканирующая калориметрия (ДСК)
- 2. 2. 8. Круговой дихроизм (КД)
- 2. 2. 9. Изотермическая титрационная калориметрия
- 3. 1. Связь между термостабильностью еШ7! и изменениями функциональной активности его мутантов
- 3. 1. 1. Тепловая денатурация еШ71 человека и его мутантов
- 3. 1. 2. Термостабильность мутантов еШ7! и его ЯР-активность
- 3. 2. Молекулярная морфология еШ7! в растворе
- 3. 3. Образование четверного комплекса еШ71*еШ73"ГТФ*
- 3. 3. 1. Взаимодействие еИРЗ с гуаниловыми нуклеотидами
- 3. 3. 2. Термодинамические параметры образование комплекса между еШ*! и еЯРЗ
- 3. 3. 3. Связывание ГТФ комплексом еИРЬеНРЗ
- 3. 3. 4. Обмен нуклеотидов в комплексе е!1Р1*е11РЗ*ГДФ/ГТФ
- 3. 3. 5. еШ71 как эффектор связывания гуаниловых нуклеотидов
- 3. 3. 6. Схема взаимодействия между еЯРЗ и его лигандами
- 3. 3. 7. Роль еШ?1 по отношению к еИРЗ
- 3. 3. 8. Роль магния в связывании гуаниловых нуклеотидов
- 3. 3. 9. Сравнение полученных результатов с данными литературы. 85 стр. 3.4.Роль индивидуальных доменов еШЧ в связывании ГТФ с еЯРЗ
- 3. 4. 1. Связывание еЯРЗ с индивидуальными доменами еИР
- 3. 4. 2. Взаимодействия между индивидуальными доменами еЯЛ
- 3. 4. 3. Связывание ГТФ с еИРЗ в присутствии М и С доменов еЯЛ
- 3. 4. 4. Роль доменов еИР1 в формировании ГТФ-связывающего центра еШ
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