Ген ключевого белка деления FtsZ микоплазмы Acholeplasma laidlawii: Клонирование, экспрессия в клетках E. coli и эволюционный консерватизм
Диссертация
Деление бактериальной клетки — сложный процесс, включающий образование перегородки деления (септы) в середине клетки на определенной стадии клеточного цикла и формирование эквивалентных дочерних клеток (Addinall, Lutkenhaus, 1996). Гены клеточного деления E. coli были идентифицированы при исследовании клеток с условными летальными мутациями. Мутации, вызывавшие формирование длинных… Читать ещё >
Содержание
- I. ВВЕДЕНИЕ
- II. ОБЗОР ЛИТЕ РАТУРЫ
- 1. Размножение клеток бактерий
- 2. Формирование сайта инициации сборки кольца деления
- 3. Белок FtsZ
- 4. Ингибирование деления клеток бактерий
- 5. Цитоскелет-подобные элементы й деление клеток микоплазм.-У
- III. МАТЕРИАЛЫ И МЕТОДЫ
- 1. Штаммы и условия культивирования
- 2. Плазмиды. Трансформация
- 3. Амплификация и клонирование
- 4. Гибридизация
- 5. Скрининг бактериальных библиотек
- 6. Сиквенс
- 7. Компьютерный анализ
- 8. Получение и очистка химерного полипептида
- 9. Получение и очистка антител
- 10. Иммуноблотинг
- IV. РЕЗУЛЬТАТЫ
- 1. Клонирование гена ftsZ A. laidlawi
- 2. Получение поликлональных антител и характеристика белков FtsZ A. laidlawii и S. citr
- 3. Экспрессия белка FtsZ A. laidlawii в клетках
- Е. col
- V. ОБСУЖДЕНИЕ
- 1. Клонирование и анализ гена и белка FtsZ микоплазмы А. 1а1с11ам1±
- 2. Сравнение последовательностей белка может использоваться для филогенетического анализа прокариот
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