Исследование структурных состояний ферментов рестрикции-модификации и их связи с каталитической активностью
Диссертация
Эксперименты по сшивке субъединиц глутаровым альдегидом наглядно продемонстрировали, в каких условиях исследованные МТазы являются мономерами, а в каких имеют олигомерную структуру. Показано, что, в отличие от Dam Т4 МТазы, фермент BamHI МТазы в свободном состоянии в растворе в мономерном виде не существует. Его преимущественная форма — димер, а также более тяжелые формы: тримеры, тетрамеры… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- ГЛАВА 1. ЛИТЕРАТУРНЫЙ ОБЗОР. СТРУКТУРНЫЕ И ФУНКЦИОНАЛЬНЫЕ ХАРАКТЕРИСТИКИ ФЕРМЕНТОВ РЕСТРИКЦИИ-МОДИФИКАЦИИ
- 1. 1. ПРИРОДА И ФУНКЦИИ ФЕРМЕНТОВ R-M СИСТЕМ
- 1. 1. 1. Обеспечение защитной функции
- 1. 1. 2. Участие в экспрессии генов
- 1. 1. 3. Функции репликации и репарации
- 1. 1. 4. Регуляция различных клеточных процессов
- 1. 2. СУЩЕСТВУЮЩИЕ ТИПЫ R-M СИСТЕМ
- 1. 2. 1. R-M системы типа I
- 1. 2. 2. R-M системы типа II
- 1. 2. 3. R-M системы типа III
- 1. 3. МЕХАНИЗМ КАТАЛИЗА МЕТИЛИРОВАНИЯ ДНК
- 1. 3. 1. Классификация ДНК — метилтрансфераз
- 1. 3. 2. ДНК -[С5-цитозин]-метилтрансферазы
- 1. 3. 3. ДНК амино-метилтрансферазы
- 1. 4. СТЕХИОМЕТРИЧЕСКИЕ ОСОБЕННОСТИ ВЗАИМОДЕЙСТВИЯ R-M СИСТЕМ ТИПА II СО СПЕЦИФИЧЕСКОЙ ПОСЛЕДОВАТЕЛЬНОСТЬЮ ДНК
- 1. 4. 1. Взаимодействие эндонуклеаз рестрикции
- 1. 4. 2. Взаимодействие МТаз
- 1. 4. 3. Исследование ДНК-ферментных взаимодействий с использованием олигонуклеотидных дуплексов
- 1. 4. 4. Кинетические исследования МТаз
- 1. 1. ПРИРОДА И ФУНКЦИИ ФЕРМЕНТОВ R-M СИСТЕМ
- 2. 1. Подготовка олигонуклеотидов
- 4. 2. Субстрат-связывающие свойства BamHI МТазы при взаимодействии с 20-членным олигонуклеотидным дуплексом
- 4. 3. Сшивка субъединиц BamHI МТазы глутаровым альдегидом
- 5. 1. Характеристики предстационарной фазы реакции в условиях насыщения Dam МТазы фага Т4 субстратами
- 5. 2. Кинетические параметры одного оборота метилирования субстрата Dam Т4 МТазой
- 5. 3. Стационарная кинетика метилирования синтетического олигонуклеотидного дуплекса BamHI МТазой
- 5. 4. Кинетические параметры одного оборота реакции метилирования BamHI метилазой
- 5. 5. Характеристики предстационарной фазы реакции в условиях насыщения BamHI МТазы субстратами
- 5. 6. Различные комплексы Dam Т4 Мазы и BamHI МТазы с субстратами и их возможная роль в реакции
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