Структурно-функциональный анализ среднего (М) домена фактора терминации трансляции eRF1 человека
Диссертация
В данной работе впервые определена структура высокого разрешения в растворе М-домена eR. Fl человека и изучены его динамические свойства. С помощью методов ЯМР нами было исследовано взаимодействие М-домена eR. Fl с большой бОБ субъединицей рибосомы эукариот, установлена его специфичность и идентифицированы аминокислотные остатки М-домена, образующие возможный интерфейс такого взаимодействия… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- ВВЕДЕНИЕ
- Глава 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Общая схема терминации трансляции
- 1. 2. Факторы терминации трансляции первого класса
- 1. 3. Факторы терминации трансляции первого класса
- 1. 4. Декодирование стоп-кодона факторами терминации трансляции первого класса
- 1. 5. Гидролиз пептидил-тРНК в пептидилтрансферазном центре
- 1. 5. 1. Молекулярная анатомия пептидил-трансферазного центра
- 1. 5. 2. Роль рРНК в реакции гидролиза пептидил-тРНК
- 1. 5. 2. Роль факторов терминации трансляции первого класса в реакции гидролиза пептидил-тРНК
- 2. 1. Материалы
- 2. 1. 1. Штаммы бактерий и плазмиды
- 2. 1. 2. Среды
- 2. 1. 3. Реактивы
- 2. 1. 4. Ферменты
- 2. 1. 5. Олигонуклеотиды
- 2. 2. Методы исследований
- 2. 2. 1. Выделение илазмидной ДНК из E. col
- 2. 2. 2. Электрофорез ДНК в агарозном геле
- 2. 2. 3. Расщепление ДНК эндонуклеазами рестрикции и реакция лигирования ДНК
- 2. 2. 4. Трансформация Е. coli плазмидной ДНК
- 2. 2. 5. Полимеразная цепная реакция (ПЦР)
- 2. 2. 6. Сайт-направленный мутагенез методом «мегапраймера»
- 2. 2. 7. Очистка фрагментов ДНК в легкоплавкой агарозе
- 2. 2. 8. Сверхэкспрессия в Е. coli и выделение мутантных форм eRFl человека
- 2. 2. 9. Сверхэкспрессия в Е. coli н выделение изолированного М- домена eRFl человека и его мутантной формы Gl83А, меченных стабильными изотопами bN и ПС
- 2. 2. 10. Электрофорез белков в денатурирующем 12% ПААГ
- 2. 2. 11. Измерение спектров ЯМР и их анализ
- 2. 2. 12. Расчет структуры
- 2. 2. 13. Анализ динамических свойств
- 2. 2. 14. ЯМР-титрование субчастицами рибосом
- 2. 2. 15. Определение активности eRFl
- 3. 1. Определение структуры М-домена eRF’l человека в растворе
- 3. 1. 1. Отнесение сигналов атомов основной белковой цепи и боковых цепей аминокислотных остатков М-домена
- 3. 1. 2. Определение вторичной и третичной структуры 58 М-домена eRF 1 человека
- 3. 1. 3. Анализ структуры М-домена eRF 1 человека
- 3. 1. 4. Влияние замены G183A в М-домене eRFl
- 3. 1. 5. Геометрия петли GGQ
- 3. 1. 6. Динамика основной цепи М-домена eRF
- 3. 2. Изучение взаимодействия между М-доменом eRFl человека и рибосомами эукариот
- 3. 2. 1. Определение способности М-домена eRFl человека специфично связываться с 60S субчастицами рибосом
- 3. 2. 2. Аминокислотные остатки М-домена eRFl, формирующие интерфейс взаимодействия белка с 60S субъединицами рибосомы
- 3. 2. 3. Влияние замены Gl83А на связывание М-домена eRFl человека с 60S субчастицами рибосом
- 3. 3. Функциональная роль аминокислотных остатков минидомена
- 3. 3. 1. Анализ первичной структуры минидомена ООС2 факторов терминации трансляции 1-го класса
- 3. 3. 2. Связывание мутантных форм еШ^ с предтерминационным комплексом и их способность вызывать конформационные перестройки
- 3. 3. 3. Анализ КБ-активности мутантных форм еШ7!
- 3. 3. 4. Возможный механизм гидролиза пептидил-тРНК в 95 пептидилтрансферазном центре рибосомы эукариот
- 3. 4. 3. аключение
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