Новые подходы к молекулярному моделированию трансмембранных доменов рецепторов, действие которых опосредовано G-белками
![Диссертация: Новые подходы к молекулярному моделированию трансмембранных доменов рецепторов, действие которых опосредовано G-белками](https://westud.ru/work/2526306/cover.png)
Диссертация
Предложенный нами эмпирический метод оценки качества упаковки ТМ-доменов — несомненно, лишь первый шаг к интегрированным высокоэффективным методикам моделирования структур МБ, пригодных для задач дизайна новых лекарственных соединений. Предстоит еще многое сделать, чтобы подход ОФМБ начал приносить практически важные результаты. Это и изучение новых классов МБ (например, p-структурных… Читать ещё >
Содержание
- Список сокращений
- Глава 1. Введение
- Глава 2. Методы предсказания структуры G-белоксопряжённых рецепторов, оценки качества упаковки белковых цепей и моделирования взаимодействий белок-ли-ганд (обзор литературы)
- 2. 1. Применение пространственных моделей белков на практике
- 2. II. Распространенные методы моделирования GPCR-рецепторов
- 2. 11. 1. Общая информация о GPCR-рецепторах
- 2. 11. 2. Методы моделирования ab initio
- 2. 11. 3. Методы моделирования на основании гомологии с родопсином
- 2. 11. 4. Возможные пути оптимизации моделей GPCR 19. 2.III. Существующие методы оценки качества упаковки белков
- 2. IV. Моделирование белок-лигандных взаимодействий
- 2. V. Методы количественной оценки гидрофобных взаимодействий
- 2. VI. Объект исследования: рецепторы мелатонина MTj и МТ
- Глава 3. Результаты и обсуждение
- 3. 1. Молекулярное моделирование рецепторов мелатонина MTj и МТ
- 3. 1. 1. Построение «стартовых» моделей рецепторов
- 3. 1. 2. Анализ и оптимизация «стартовых» моделей
- 3. 1. 3. Выбор оптимального угла вращения а-спирали ТМЗ
- 3. 1. 4. Структурные различия сайтов МТХ и МТ2, модель селективности
- 3. 1. 5. Выводы (I) 40 3.II. Разработка нового метода оценки качества упаковки трансмембранных а-спиральных доменов белков
- 3. 11. 1. Подготовка и характеристика набора структурных данных
- 3. 11. 2. «Оценивающая функция для мембранных белков»
- 3. 11. 3. Тестирование метода
- 3. 11. 4. Выводы (II) 57 3.III. Новый подход к улучшению результатов докинга
- 3. III.1. Комплементарность гидрофобных свойств в кристаллографических комплексах белок-лиганд
- 3. HI.2. Применение численной оценки гидрофобного соответствия для улучшения результатов докинга
- 3. III.3. Выводы (III)
- 3. 1. Молекулярное моделирование рецепторов мелатонина MTj и МТ
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