Разработка технологии производства набора реагентов для выявления антител к вирусу трансмиссивного гастроэнтерита свиней иммуноферментным методом
Диссертация
С появлением (1984) и распространением природного делеционного мутанта ВТГС — респираторного коронавируса свиней (РКВС), в европейских странах значительно сократились вспышки трансмиссивного гастроэнтерита свиней и циркуляция вируса ТГС (В.А.Сергеев с соавт., 1989; D.J. Garwes et al., 1988; H.W.Moon, 1994; T.S.Mor et al., 1998; L. Kim et al., 2000; R.D.Wesley, K.M.Lager, 2003 и др.). Однако… Читать ещё >
Содержание
- 1. ВВЕДЕНИЕ
- 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Трансмиссивный гастроэнтерит свиней (ТГС): общая характеристика болезни
- 2. 2. Биологические особенности вируса ТГС
- 2. 3. Особенности иммунитета при ТГС
- 2. 4. Методы лабораторной диагностики и средства специфической профилактики ТГС
- СОБСТВЕННЫЕ ИССЛЕДОВАНИЯ
- 3. МАТЕРИАЛЫ И МЕТОДЫ
- 3. 1. Вирусный антиген
- 3. 2. Моноклональные антитела
- 3. 3. Испытуемые пробы биологического материала
- 3. 4. Получение конъюгата МкА 6С7 с пероксидазой хрена
- 3. 5. Методы иммуноферментного анализа. 3.6. Реакция нейтрализации вируса. з
- 4. 3.7. Коммерческие ИФА-наборы
- 3. 8. Электрофорез в ПААГ-ДСН
- 3. 9. Иммуноблоттинг
- 3. 10. Статистическая обработка результатов
- 4. РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЙ
- 4. 1. Разработка непрямого варианта твердофазного ИФА на основе моноклональных антител для выявления антител к вирусу трансмиссивного гастроэнтерита свиней
- 4. 1. 1. Получение и характеристика иммунологических реагентов, предназначенных для использования в ИФА
- 4. 1. 2. Разработка иммуноферментной тест-системы для выявления антител к вирусу ТГС. л 4.1.3. Оценка диагностических параметров разработанной иммуноферментной тест-системы
- 4. 1. 3. 1. Определение антител к вирусу ТГС в сыворотке крови животных
- 4. 1. 3. 2. Определение антител к вирусу ТГС в молозиве иммунных свиноматок
- 4. 1. 3. 3. Оценка возможности тест-системы дифференцировать антитела к вирусу ТГС от антител к РКВС
- 4. 1. Разработка непрямого варианта твердофазного ИФА на основе моноклональных антител для выявления антител к вирусу трансмиссивного гастроэнтерита свиней
- 4. 2. Разработка технологии изготовления ИФА-набора для выявления антител к вирусу трансмиссивного гастроэнтерита свиней «ТГС-СЕРОТЕСТ». ^
- 4. 2. 1. Определение стабильности специфических компонентов ИФА-набора при хранении
- 4. 2. 2. Разработка оптимальной комплектации ИФА-набора и инструкции по его применению
- 4. 2. 3. Применение ИФА-набора «ТГС-СЕРОТЕСТ» в практических условиях
- 4. 2. 4. Контроль воспроизводимости результатов, полученных с помощью ИФА — набора «ТГС-СЕРОТЕСТ»
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