ΠΠ»Π΅ΡΠΎΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ Π½Π΅ΠΉΡΠΎΡΠΈΠ±ΡΠΈΠ»Π»ΡΡΠ½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ° ΠΈ FTDP-17 Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ ΡΠΎΡΠΌ Π±Π΅Π»ΠΊΠ° tau
![ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ: ΠΠ»Π΅ΡΠΎΡΠ½Π°Ρ ΠΌΠΎΠ΄Π΅Π»Ρ Π½Π΅ΠΉΡΠΎΡΠΈΠ±ΡΠΈΠ»Π»ΡΡΠ½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΠ»ΡΡΠ³Π΅ΠΉΠΌΠ΅ΡΠ° ΠΈ FTDP-17 Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΡ
ΡΠΎΡΠΌ Π±Π΅Π»ΠΊΠ° tau](https://westud.ru/work/2515990/cover.png)
ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ
ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π²ΡΡΠΎΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠ΅ Π°Π³ΡΠ΅Π³Π°ΡΡ Π±Π΅Π»ΠΊΠ° tau Π½Π°Π±Π»ΡΠ΄Π°ΡΡΡΡ Π² ΡΠ°ΡΠΊΠΎΠ·ΠΈΠ»-Π½Π΅ΡΠ°ΡΡΠ²ΠΎΡΠΈΠΌΠΎΠΉ ΡΡΠ°ΠΊΡΠΈΠΈ ΡΠΎΠ»ΡΠΊΠΎ Π»ΠΈΠ½ΠΈΠΉ Π18 ΠΈ Π18/ΠΠ280 ΠΈ ΡΠΊΠΎΡΠΎΡΡΡ ΠΈΡ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ. ΠΠΎΠ»ΡΡΠ΅Π½Ρ ΠΈ ΠΎΡ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½Ρ ΡΡΠ°Π±ΠΈΠ»ΡΠ½ΡΠ΅ ΠΈΠ½Π΄ΡΡΠΈΠ±Π΅Π»ΡΠ½ΡΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ Π½Π΅ΠΉΡΠΎΠ±Π»Π°ΡΡΠΎΠΌΡ ΠΌΡΡΠΈ (N2a (tet ΠΎΠΏ)), ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΡΡΠΈΠ΅ ΠΌΡΡΠ°Π½ΡΠ½ΡΠ΅ ΡΠΎΡΠΌΡ Π±Π΅Π»ΠΊΠ° tau (Kl8- Π18/ΠΠ280- Π18/ΠΠ280/1277Π /308Π ). ΠΡΠ΅Π΄Π»ΠΎΠΆΠ΅Π½Π½Π°Ρ… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. 1. ΠΠΠΠΠΠΠ¬ ΠΠΠ¬Π¦ΠΠΠΠΠΠ Π
- 1. 2. Π€Π ΠΠΠ’Π-Π’ΠΠΠΠΠ ΠΠΠ¬ΠΠ«Π ΠΠΠΠΠΠ¦ΠΠ Π ΠΠΠ ΠΠΠΠ‘ΠΠΠΠΠ, Π‘ΠΠ―ΠΠΠΠΠ«Π Π‘ Π₯Π ΠΠΠΠ‘ΠΠΠΠ
- 1. 3. ΠΠΠΠΠ’ΠΠΠ« ΠΠΠΠ ΠΠΠΠΠ¬ΠΠΠ ΠΠΠΠΠΠΠ ΠΠ¦ΠΠ ΠΠ Π ΠΠΠΠΠΠΠ ΠΠΠ¬Π¦ΠΠΠΠΠΠ Π Π Π€Π ΠΠΠ’Π-Π’ΠΠΠΠΠ ΠΠΠ¬ΠΠ«Π₯ΠΠΠΠΠΠ¦ΠΠ―Π₯, Π‘ΠΠ―ΠΠΠΠΠ«Π₯ Π‘ Π₯Π ΠΠΠΠ‘ΠΠΠΠ
- 1. 4. ΠΠ‘Π‘ΠΠ¦ΠΠΠ ΠΠΠΠΠΠ«Π Π‘ ΠΠΠΠ ΠΠ’Π Π£ΠΠΠ§ΠΠΠΠ ΠΠΠΠΠ TAU
- 1. 4. 1. ΠΠ΅Π»ΠΊΠΈ, Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ Ρ ΠΌΠΈΠΊΡΠΎΡΡΡΠ±ΠΎΡΠΊΠ°ΠΌΠΈ
- 1. 4. 2. Π‘ΡΡΡΠΊΡΡΡΠ° Π±Π΅Π»ΠΊΠ° tau
- 1. 4. 3. Π€ΡΠ½ΠΊΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° tau
- 1. 5. ΠΠΠ ΠΠΠΠΠΠΠΠ ΠΠΠ ΠΠΠΠ’ΠΠ ΠΠΠΠ TAU
- 2. Π¦ΠΠΠ Π ΠΠΠΠΠ§Π ΠΠ‘Π‘ΠΠΠΠΠΠΠΠΠ―
- 3. ΠΠΠ’ΠΠ ΠΠΠΠ« Π ΠΠΠ’ΠΠΠ«
- 3. 1. ΠΠΠΠ ΠΠΠΠΠΠΠΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠ«
- 3. 1. 1. ΠΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ
- 3. 1. 2. ΠΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΡΠ»Π΅ΠΊΡΡΠΎΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 3. 1. 3. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΊΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠ½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ
- 3. 2. ΠΠΠΠΠΠ£ΠΠ―Π ΠΠ-ΠΠΠΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠ«
- 3. 2. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ
- 3. 2. 1. 1. ΠΠΈΠ½ΠΈΠΏΡΠ΅ΠΏ
- 3. 2. 1. 2. ΠΠ°ΠΊΡΠΈΠΏΡΠ΅ΠΏ
- 3. 2. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ Π³Π΅Π½ΠΎΠΌΠ½ΠΎΠΉ ΠΠΠ ΠΈΠ· ΡΠΊΠ°Π½ΠΈ Ρ Π²ΠΎΡΡΠ° ΠΌΡΡΠΈ
- 3. 2. 3. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠΠ
- 3. 2. 4. ΠΠ΅Π»Ρ-ΡΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅Π· ΠΠΠ
- 3. 2. 5. ΠΠΎΠ΄ΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΠΠ
- 3. 2. 5. 1. Π Π°ΡΡΠ΅ΠΏΠ»Π΅Π½ΠΈΠ΅ ΠΠΠ ΡΠ½Π΄ΠΎΠ½ΡΠΊΠ»Π΅Π°Π·Π°ΠΌΠΈ ΡΠ΅ΡΡΡΠΈΠΊΡΠΈΠΈ
- 3. 2. 5. 2. ΠΠ΅ΡΠΎΡΡΠΎΡΠΈΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ CIP-ΡΠΎΡΡΠ°ΡΠ°Π·ΠΎΠΉ
- 3. 2. 5. 3. ΠΠΌΠΏΠ»ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ Π² ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ
- 3. 2. 5. 4. Π’ΠΠ Π-ΠΊΠ»ΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 3. 2. 5. 5. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΠΠ ΠΈΠ· Π°Π³Π°ΡΠΎΠ·Π½ΡΡ Π³Π΅Π»Π΅ΠΉ
- 3. 2. 5. 6. ΠΠΈΠ³ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅
- 3. 2. 6. Π‘Π΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠΠ
- 3. 2. 1. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΠΎΠΉ ΠΠΠ
- 3. 3. ΠΠ£ΠΠ¬Π’Π£Π Π« ΠΠΠΠ’ΠΠ
- 3. 3. 1. ΠΠ»Π΅ΡΠΎΡΠ½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ
- 3. 3. 2. ΠΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΡΠ΅Π΄Ρ ΠΈ ΠΏΠ°ΡΡΠ°ΠΆΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ
- 3. 3. 3. ΠΠ°ΠΌΠΎΡΠ°ΠΆΠΈΠ²Π°Π½ΠΈΠ΅ ΠΈ ΠΎΡΡΠ°ΠΈΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ
- 3. 3. 4. ΠΠΎΠ΄ΡΡΠ΅Ρ ΠΊΠ»Π΅ΡΠΎΠΊ Π² Π³Π΅ΠΌΠΎΡΠΈΡΠΎΠΌΠ΅ΡΡΠ΅
- 3. 3. 5. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΡΡΠΈΠ½ΡΡ Π½Π΅ΠΉΡΠΎΠ½ΠΎΠ²
- 3. 3. 6. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΈ ΠΊΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΌΡΡΠΈΠ½ΡΡ ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠ²
- 3. 3. 7. Π‘ΡΠ°Π±ΠΈΠ»ΡΠ½Π°Ρ ΡΡΠ°Π½ΡΡΠ΅ΠΊΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ Ρ ΠΏΠΎΠΌΠΎΡΡΡ DOT ΠΠ
- 3. 3. 8. ΠΠΌΠΌΡΠ½ΠΎΡΠ»ΡΠΎΡΠ΅ΡΡΠ΅Π½ΡΠΈΡ
- 3. 3. 9. Π’Π΅ΡΡ Π½Π° Π»ΡΡΠΈΡΠ΅ΡΠ°Π·Ρ
- 3. 4. ΠΠΠΠ₯ΠΠΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠ«
- 3. 4. 1. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π±Π΅Π»ΠΊΠ° ΠΏΠΎ ΠΡΡΠ΄ΡΠΎΡΠ΄Ρ
- 3. 4. 2. ΠΡΠΈΠ³ΠΎΡΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ Π»ΠΈΠ·Π°ΡΠΎΠ²
- 3. 4. 3. ΠΠΊΡΡΡΠ°ΠΊΡΠΈΡ Π±Π΅Π»ΠΊΠ° ΠΏΠΎ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΡΠ²ΠΈΡΠ°
- 3. 4. 4. ΠΠ»Π΅ΠΊΡΡΠΎΡΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΡΠ°Π·Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π±Π΅Π»ΠΊΠΎΠ² Π² ΠΏΠΎΠ»ΠΈΠ°ΠΊΡΠΈΠ»Π°ΠΌΠΈΠ΄Π½ΠΎΠΌ Π³Π΅Π»Π΅
- 3. 4. 5. ΠΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π³Π΅Π»Π΅ΠΉ Coomassie Brilliant Blue R
- 3. 4. 6. ΠΠ΅ΡΡΠ΅ΡΠ½ Π±Π»ΠΎΡ
- 3. 4. 7. ΠΠΌΠΌΡΠ½Π½ΠΎΠ΅ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ ΠΈ Π½Π΅Π³Π°ΡΠΈΠ²Π½ΠΎΠ΅ ΠΊΠΎΠ½ΡΡΠ°ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠ΅ΠΈΠ½ΠΎΠ²
- 3. 1. ΠΠΠΠ ΠΠΠΠΠΠΠΠΠ§ΠΠ‘ΠΠΠ ΠΠΠ’ΠΠΠ«
- 4. 1. ΠΠΠΠΠΠ ΠΠΠΠΠΠ
- 4. 2. Π‘Π’ΠΠΠΠΠ¬ΠΠΠ― ΠΠΠ‘ΠΠ ΠΠ‘Π‘ΠΠ― Π18, Π18/ΠΠ280, Π18/ΠΠ280/Π¨7Π /Π08Π
- 4. 2. 1. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π18 ΠΈ ΠΌΡΡΠ°Π½ΡΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
- 4. 2. 2. ΠΠ°Π²ΠΈΡΠΈΠΌΠΎΡΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ tau-rera ΠΎΡ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ Π΄ΠΎΠΊΡΠΈΡΠΈΠΊΠ»ΠΈΠ½Π°
- 4. 2. 3. ΠΠ³ΡΠ΅Π³Π°ΡΠΈΡ tau
- 4. 2. 4. ΠΠ»Π΅ΠΊΡΡΠΎΠ½Π½Π°Ρ ΠΌΠΈΠΊΡΠΎΡΠΊΠΎΠΏΠΈΡ
- 4. 2. 5. ΠΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π²ΡΡΠΎΠΊΠΎΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΡ Π°Π³ΡΠ΅Π³Π°ΡΠΎΠ² tau Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΈΠΎΡΠ»Π°Π²ΠΈΠ½Π° S
- 4. 2. 6. ΠΠ½Π³ΠΈΠ±ΠΈΡΠΎΠ²Π°Π½Π½Π΅ ΠΈΠ½Π΄ΡΠΊΡΠΈΠΈ ΠΏΡΡΠ΅ΠΌ ΡΠ΄Π°Π»Π΅Π½ΠΈΡ Π΄ΠΎΠΊΡΠΈΡΠΈΠΊΠ»ΠΈΠ½Π° ΠΈΠ· ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΡΠ΅Π΄Ρ
- 4. 3. Π‘ΠΠ ΠΠΠΠΠ ΠΠΠΠΠ Π‘Π’ΠΠΠΠΠ«Π₯ ΠΠΠ©ΠΠ‘Π’Π
- 4. 4. ΠΠΠ ΠΠΠ§ΠΠ«Π ΠΠΠΠ ΠΠΠΠ«Π ΠΠ£ΠΠ¬Π’Π£Π Π«
- 4. 5. ΠΠ«Π―ΠΠΠΠΠΠ Π‘ΠΠ ΠΠΠΠΠ-ΠΠΠ ΠΠ‘Π’ΠΠΠ ΠΠΠ«Π₯ ΠΠΠ ΠΠΠΠ’ΠΠ IN VJVO
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