Пространственная структура медных полиядерных оксидаз — лакказ Coriolus zonatus и Cerrena maxima
Диссертация
Термин «полимедные голубые белки» (ПМГБ) используется для группы ферментов, включающих нитритредуктазы и полимедные оксидазы (ПМО). ПМГБ состоят из тандема повторяющихся аналогичных по своей последовательности доменов, которые в некоторых аспектах гомологичны однодоменным белкам купредоксинового ряда, к которым относятся пластоцианин, азурин, псевдоазурин, рустицианин, стеллацианин и амицианин… Читать ещё >
Содержание
- 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Полимедные голубые белки
- 1. 2. Пути эволюции полимедных голубых белков (ПМГБ)
- 1. 3. Медьсвязывающие сайты ПМГБ
- 1. 4. Трехдоменные ПМГБ
- 1. 5. Лакказы
- 1. 5. 1. Белая лакказа
- 1. 5. 2. Лакказы насекомых
- 1. 5. 3. Малые грибные лакказы
- 1. 6. Реакции, катализируемые лакказами
- 1. 7. Биологическое распространение и функция лакказы
- 1. 8. Химический состав фермента
- 1. 8. 1. Выделение лакказы
- 1. 8. 2. Молекулярная масса и аминокислотная последовательность
- 1. 8. 3. Содержание углеводов
- 1. 8. 4. Содержание металла и три типа ионов меди
- 1. 8. 5. Обратимое удаление меди
- 1. 9. Спектроскопические и магнитные свойства лакказ
- 1. 9. 1. Оптические спектры
- 1. 9. 2. Исследования рентгеновской абсорбции
- 1. 9. 3. Магнитная восприимчивость
- 1. 10. Окислительно- восстановительные свойства
- 1. 11. Каталитическая реакция
- 2. МАТЕРИАЛЫ И МЕТОДЫ
- 2. 1. МАТЕРИАЛЫ
- 2. 1. 1. Биологические материалы
- 2. 1. 2. Химические материалы
- 2. 1. 3. Носители
- 2. 2. МЕТОДЫ
- 2. 2. 1. КРИСТАЛЛИЗАЦИЯ БИОМАКРОМОЛЕКУЛ
- 2. 2. 2. Метод молекулярного замещения
- 2. 2. 3. Уточнение атомной структуры
- 2. 2. 4. Корректность решённой структуры
- 2. 1. МАТЕРИАЛЫ
- 3. 1. Получение препаратов лакказ
- 3. 1. 1. Выращивание гриба Coriolus zonaius в колбах
- 3. 1. 2. Очистка лакказы Coriolus zonaius
- 3. 1. 3. Поверхностное культивирование Cerrena maxima
- 3. 1. 4. Глубинное культивирование Cerrena maxima
- 3. 1. 5. Очистка лакказы Cerrena maxima
- 3. 2. Кристаллизация лакказ
- 3. 2. 1. Кристаллизация лакказы Coriolus zonaius
- 3. 2. 2. Кристаллизация лакказы Cerrena maxima
- 3. 3. Получение наборов рентгенодифракционных данных
- 3. 3. 1. Набор интенсивностей дифрагированных кристаллом лакказы
- 3. 3. 2. Экспериментальный набор интенсивностей дифрагированных кристаллом лакказы Cerrena maxima
- 3. 4. Построение стартовой модели
- 3. 4. 1. Построение стартовой модели структуры лакказы Coriolus zonatus
- 3. 4. 2. Построение стартовой модели структуры лакказы Cerrena maxima
- 3. 5. Уточнение структуры
- 3. 5. 1. Уточнение структуры лакказы Coriolus zonatus
- 3. 5. 2. Уточнение структуры лакказы Cerrena maxima
- 4. 1. Получение биоматериала для выделения лакказы Coriolus zonatus
- 4. 2. Выделение лакказы Coriolus zonatus
- 4. 3. Получение биомассы для выделения лакказы Cerrena maxima
- 4. 3. 1. Поверхностное культивирование Cerrena maxima
- 4. 3. 2. Глубинное культивирование Cerrena maxima
- 4. 4. Очистка лакказы Cerrena maxima
- 4. 5. Кристаллизация лакказы
- 4. 5. 1. Кристаллизация лакказы Coriolus zonatus
- 4. 5. 2. Кристаллизация лакказы Cerrena maxima
- 4. 6. Получение дифракционных данных с кристаллов лакказы
- 4. 6. 1. Получение наборов дифракционных данных от кристаллов лакказы Coriolus zonatus
- 4. 7. Построение стартовой модели для решения и уточнения структуры лакказы Coriolus zonatus с разрешением 3.2 A
- 4. 8. Получение наборов дифракционных данных от кристаллов лакказы
- 4. 9. Построение стартовой модели для решения и уточнения структуры лакказы Cerrena maxima
- 4. 10. Уточнение структуры лакказы Coriolus zonatus
- 4. 11. Уточнение структуры лакказы Cerrena maxima
- 4. 12. Первичная структура молекул лакказ
- 4. 13. Пространственная организация молекул лакказ
- 4. 14. Структура активного центра
- 4. 15. Водные каналы
- 4. 16. Углеводная компонента лакказы Cerrena maxima
- 4. 17. Особенности пространственной структуры Cerrena maxima
- 4. 18. Предполагаемый механизм действия фермента
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- Автор приносит глубокую благодарность:
- Всему коллективу Лаборатории белковой кристаллографии Института кристаллографии имени A.B. Шубникова РАН за моральную поддержку.
- Е.В. Степановой и A.B. Королевой из института биохимии имени А. Н. Баха РАН за предоставленную возможность и помощь при выделении и очистке белков-
- W. Voelter, Ch. Betzel и B.C. Ламзину за предоставленную возможность работы на синхротронном источнике DEZY (Гамбург, Германия) и Качаловой Г. С. за помощь при получении наборов рентгенодифракционных интенсивностей-
- Н.Е. Жухлистовой и А. Г. Габдулхакову за неоценимую помощь на всех этапах выполнения данной работы-
- A.M. Михайлову за предложенную тему, руководство работой, неоценимую помощь и поддержку.