Получение в E. coli, ренатурация и исследование внемембранных доменов холинорецепторов
Диссертация
В связи с этим, нами были поставлены задачи разработать условия экспрессии внемембранных (экстрацеллюлярных и цитоплазматических) доменов субъединиц никотинового ацетилхолинового рецептора с последующей ренатурацией очищенных продуктов и изучения физико-химических свойств доменов. В качестве объектов исследования мы выбрали экстрацеллюлярные домены мышечного АХР а-субъединиц Torpedo и человека… Читать ещё >
Содержание
- СПИСОК ИСПОЛЬЗУЕМЫХ СОКРАЩЕНИЙ
- 1. ЛИТЕРАТУРНЫЙ ОБЗОР
- 1. 1. Использование ЯМР спектроскопии на различных ядрах
- 1. 2. Биосинтез белков, содержащих фтораминокислоты
- 1. 3. I9 °F ЯМР исследования белков, содержащих остатки фторароматических аминокислот
- 2. РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЕ
- 2. 1. Экстрацеллюлярный фрагмент а-субъединицы Torpedo californica а (1 -209) и его мутант [Trp60Phe]-a (1−209)
- 2. 2. Фторсодержащие аналоги экстрацеллюлярного фрагмента а-субъединицы Torpedo californica a (l-209)F и его мутанта [Trp60Phe]-a (l-209)F
- 2. 3. Две формы экстрацеллюлярного домена а-субъединицы мышечного АХР человека
- 2. 4. Экстрацеллюлярный фрагмент а7-субъединицы крысы а7(7−208) и его мутант [Cysl 16Ser, Cysl90Ser, Cysl91Ser]-a (l-209)
- 2. 5. Цитоплазматический фрагмент 6-субъединицы Torpedo californica 8(313−455)
- 3. ЭКСПЕРИМЕНТАЛЬНАЯ ЧАСТ
- ВЫВОДЫ
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