Роль конформационных изменений в функционировании неорганической пирофосфатазы Escherichia coli
Диссертация
Определены значения энтальпии денатурации нативной РРазы E. coli, мутантных вариантовt GlylOOAla, Glyl47Val и ряда вариантов с единичными заменами в различных областях молекулы РРазы. Показано, что в присутствии 10 мМ Mg2+ энтальпия денатурации нативной РРазы возрастает на 1140 кДж/моль. Рассмотрены две модели протекания химической денатурации нативной РРазы E. coli и мутантных РРаз GlylOOAla… Читать ещё >
Содержание
- 1. Введение
- 2. Роль петель в функционировании белков (обзор литературы)
- 2. 1. Конформационные изменения в белковых структурах
- 2. 2. Строение петель
- 2. 3. Конформационные изменения петель и их участие в функционировании белков
- 2. 4- Роль остатков глицина в формировании структуры и обеспечении г конформационной подвижности активного центра
- 3. Конформационные изменения в структуре неорганической пирофосфатазы обзор литературы)
- 3. 1. Связывание ионов металла
- 3. 2. Связывание субстрата
- 3. 3. Гидролиз субстрата
- 3. 4. Уход продуктов
- 4. Роль конформационных изменений в функционировании неорганической пирофосфатазы Escherichia coli (обсуждение результатов)
- 4. 1- Исследование мутантных вариантов Е-РРазы GlylOOAla и Glyl47Val
- 4. 2. Количественная оценка стабильности структуры РРазы
- 4. 3. Роль остатка Asp67 в функционировании РРазы Е. col
- 5. Экспериментальная часть
- Выводы
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