ΠΠΈΠ΄ΠΎΠ²ΠΎΠΉ ΡΠΎΡΡΠ°Π² Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales Π² ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΠ΅ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ° Ρ Π·Π΄ΠΎΡΠΎΠ²ΡΡ Π»ΡΠ΄Π΅ΠΉ ΠΈ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ, Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΠΈΠ· B.uniformis
ΠΠΈΡΡΠ΅ΡΡΠ°ΡΠΈΡ
Π‘ΠΎΠ΄Π΅ΡΠΆΠΈΠΌΠΎΠ³ΠΎ ΡΠΎΠ»ΡΡΠΎΠΉ ΠΊΠΈΡΠΊΠΈ Π΄ΠΎΡΡΠΈΠ³Π°Π΅Ρ 10 ΠΌΠΈΠΊΡΠΎΠ±Π½ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ. ΠΡΠ΅ΠΎΠ±Π»Π°Π΄Π°ΡΡΠΈΠ΅ Π² ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ Π² ΡΡΠΎΠΉ ΡΠΊΠΎΡΠΈΡΡΠ΅ΠΌΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΈ ΠΏΡΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ°Ρ ΠΊ ΠΏΠΎΡΡΠ΄ΠΊΠ°ΠΌ Bacteroidales (ΡΠΈΠΏ Bacteroidetes, ΠΊΠ»Π°ΡΡ Bacteroidia) ΠΈ Clostridials (ΡΠΈΠΏ Firmicutes, ΠΊΠ»Π°ΡΡ Clostridia) ΠΈ ΡΠΎΠ΄Ρ Bifidobacterium (ΡΠΈΠΏ Actinobacteria, ΠΊΠ»Π°ΡΡ Actinobacteria, ΠΏΠΎΡΡΠ΄ΠΎΠΊ Bifidobacteriales). Π ΠΏΠΎΡΡΠ΄ΠΊΡ Bacteroidales ΠΎΡΠ½ΠΎΡΡΡΡΡ ΡΡΡΠΎΠ³ΠΎ Π°Π½Π°ΡΡΠΎΠ±Π½ΡΠ΅… Π§ΠΈΡΠ°ΡΡ Π΅ΡΡ >
- Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΡΠ΄Π΅ΡΠΆΠΊΠ°
- ΠΠΈΡΠ΅ΡΠ°ΡΡΡΠ°
- ΠΡΡΠ³ΠΈΠ΅ ΡΠ°Π±ΠΎΡΡ
- ΠΠΎΠΌΠΎΡΡ Π² Π½Π°ΠΏΠΈΡΠ°Π½ΠΈΠΈ
Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅
- ΠΠΠΠΠ 1. ΠΠΠΠΠ ΠΠΠ’ΠΠ ΠΠ’Π£Π Π«
- 1. 1. ΠΠ²Π΅Π΄Π΅Π½ΠΈΠ΅
- 1. 2. ΠΠ½Π°ΡΠ΅Π½ΠΈΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales Π² ΠΌΠΈΠΊΡΠΎΠ±Π½ΠΎΡΠ΅Π½ΠΎΠ·Π΅ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ°
- 1. 3. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales Π² ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΠ΅ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ°
- 1. 4. ΠΠ»Π°Π·ΠΌΠΈΠ΄Ρ Ρ Π±Π°ΠΊΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ²
- ΠΠΠΠΠ 2. ΠΠΠ’ΠΠ ΠΠΠΠ« Π ΠΠΠ’ΠΠΠ«
- 2. 1. ΠΠ±ΡΠ΅ΠΊΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ
- 2. 2. ΠΠ°ΠΊΡΠ΅ΡΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ, ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½Π½ΡΠ΅ Π΄Π»Ρ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales
- 2. 2. 1. ΠΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ
- 2. 2. 2. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ ΠΈΠ· ΡΡΠ°ΠΌΠΌΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ²
- 2. 2. 3. Π Π΅ΡΡΡΠΈΠΊΡΠΈΠΎΠ½Π½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· Π°ΠΌΠΏΠ»ΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ ΡΠΈΠ±ΠΎΡΠΎΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ (ARDRA, Amplified Ribosomal DNA Restriction Analysis)
- 2. 2. 4. Π‘Π΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° Π³Π΅Π½Π° 16S ΡΠ ΠΠ
- 2. 3. ΠΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ Π±ΠΈΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ, ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½Π½ΡΠ΅ Π΄Π»Ρ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΡ ΠΈ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄ Ρ Π±Π°ΠΊΡΠ΅ΡΠΎΠΈΠ΄ΠΎΠ²
- 2. 3. 1. ΠΡΠ»ΡΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ
- 2. 3. 2. ΠΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½Π°Ρ ΡΠ΅ΠΏΠ½Π°Ρ ΡΠ΅Π°ΠΊΡΠΈΡ
- 2. 3. 3. ΠΠ±ΡΠ°Π±ΠΎΡΠΊΠ° ΠΠΠ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΠΌΠΈ Π½ΡΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΠ΅Π½Π°
- 2. 3. 4. Π‘ΠΈΠ½ΡΠ΅Π· ΠΠΠ-ΠΎΠ»ΠΈΠ³ΠΎΠ½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄ΠΎΠ²
- 2. 3. 5. Π’ΡΠ°Π½ΡΡΠΎΡΠΌΠ°ΡΠΈΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΡ ΡΡΠ°ΠΌΠΌΠΎΠ²
- 2. 3. 6. ΠΠΎΠ½ΡΡΠ³Π°ΡΠΈΡ
- 2. 3. 7. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡΡ ΠΠΠ
- 2. 3. 8. Π‘Π΅ΠΊΠ²Π΅Π½ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ
- 2. 3. 9. ΠΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΡΠ°Π»ΡΠ½ΠΎΠΉ Π ΠΠ
- 2. 3. 10. ΠΠ’-ΠΠ¦Π Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ
- 2. 3. 11. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π°Π½ΡΠΈΠ±ΠΈΠΎΡΠΈΠΊΠΎΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΎΡΡΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π±ΡΠΌΠ°ΠΆΠ½ΡΡ Π΄ΠΈΡΠΊΠΎΠ² ΠΈ ΠΏΠΎΠΈΡΠΊ Π³Π΅Π½Π° tetQ
- 2. 4. ΠΠ΅ΡΠΎΠ΄Ρ Π±ΠΈΠΎΠΈΠΈΡΠΎΡΠΌΠ°ΡΠΈΠΊΠΈ ΠΈ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ Π΄Π°Π½Π½ΡΡ
- 2. 4. 1. ΠΠ΅ΡΠΎΠ΄Ρ Π±ΠΈΠΎΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΊΠΈ
- 2. 4. 2. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° Π΄Π°Π½Π½ΡΡ
- 3. 1. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΌΠΈΠΊΡΠΎΡΠ»ΠΎΡΡ ΠΊΠΈΡΠ΅ΡΠ½ΠΈΠΊΠ°
- 3. 2. ΠΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ Π²ΠΈΠ΄ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΎΡΡΠ°Π²Π° ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»Π΅ΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales
- 3. 3. ΠΠΎΠΈΡΠΊ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Π½ΡΡ ΠΠΠ Π² ΡΡΠ°ΠΌΠΌΠ°Ρ Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales
- 3. 4. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠΎΠ»Π½ΠΎΠΉ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pBUN
- 3. 5. ΠΠ½Π°Π»ΠΈΠ· ΠΏΠΎΠ»Π½ΠΎΠΉ Π½ΡΠΊΠ»Π΅ΠΎΡΠΈΠ΄Π½ΠΎΠΉ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pBUN
- 3. 6. ΠΠΎΠΈΡΠΊ ΠΎΡΠΊΡΡΡΡΡ ΡΠ°ΠΌΠΎΠΊ ΡΡΠΈΡΡΠ²Π°Π½ΠΈΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pBUN24 ΡΡΠ΅Π΄ΠΈ Π΄ΡΡΠ³ΠΈΡ ΡΡΠ°ΠΌΠΌΠΎΠ² Π±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΠΏΠΎΡΡΠ΄ΠΊΠ° Bacteroidales
- 3. 7. ΠΠΎΠ²ΡΠΎΡΠΎΠ΅ Π²ΡΠ΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pBUN
- 3. 8. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΎΡΠΊΡΡΡΡΡ ΡΠ°ΠΌΠΎΠΊ ΡΡΠΈΡΡΠ²Π°Π½ΠΈΡ ΠΏΠ»Π°Π·ΠΌΠΈΠ΄Ρ pBUN24 Π² ΡΡΠ°ΠΌΠΌΠ°Ρ Bacteroides vulgatus ΠΈ Parabacteroides distasonis
- 3. 9. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΡΠ²ΡΡΠ²ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΠΊ Π°Π½ΡΠΈΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ°ΠΌ
- 3. 10. ΠΠΎΠ½ΡΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅Π»Π½ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΊΠ»ΠΎΠ½ΠΈΡΡΡΡΠ΅Π³ΠΎ Π²Π΅ΠΊΡΠΎΡΠ° E. coli-Bacteroides
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