Конструирование иммунной комбинаторной библиотеки одноцепочечных антител человека и отбор из нее вируснейтрализующих антител против ортопоксвирусов
Диссертация
Открытие Kohler и Milstein гибридомной технологии в 1975 году позволило нарабатывать моноклональные антитела грызунов разной специфичности. С тех пор гибридомы стали основным источником моноклональных антител (МАТ), использующихся в фундаментальных исследованиях и в биотехнологии при создании тест-систем. Но при использовании мышиных МАТ в терапии оказалось, что они имеют ряд недостатков, среди… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- ГЛАВА 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. СТРУКТУРА ИММУНОГЛОБУЛИНОВ И ИХ ГЕНОВ
- 1. 1. 1. Структура антител
- 1. 1. 2. Гены иммуноглобулинов
- 1. 1. 3. Реорганизация генов иммуноглобулинов в ходе дифференцировки В-лимфоцитов
- 1. 2. ПОЛУЧЕНИЕ ФРАГМЕНТОВ АНТИТЕЛ ЧЕЛОВЕКА С ПОМОЩЬЮ ФАГОВОГО ДИСПЛЕЯ
- 1. 2. 1. Фаговый дисплей
- 1. 2. 1. 1. Фрагменты антител, используемые для фагового дисплея
- 1. 2. 1. 2. Фаги, используемые для дисплея фрагментов антител
- 1. 2. 2. Комбинаторные библиотеки антител человека
- 1. 2. 2. 1. Конструирование наивных библиотек
- 1. 2. 2. 2. Конструирование синтетических библиотек
- 1. 2. 2. 3. Конструирование иммунных библиотек
- 1. 2. 1. Фаговый дисплей
- 1. 1. СТРУКТУРА ИММУНОГЛОБУЛИНОВ И ИХ ГЕНОВ
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