Анализ трансренальной ДНК как новый подход к диагностике злокачественных новообразований
Диссертация
Присутствие ДНК в сыворотке было продемонстрировано в ряде ранних работ. Концентрация ДНК в сыворотке отражает интенсивность клеточной гибели в организме и существенно повышается при ряде патологических процессов, включая онкологические заболевания. Поскольку опухоли обычно слабо васкуляризированы, то распад входящих в их состав клеток весьма вероятен, чему есть много экспериментальных… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- 1. ОБЗОР ЛИТЕРАТУРЫ
- 1. 1. Генетическая и эпигенетическая составляющие 11 канцерогенеза
- 1. 2. Маркеры — наиболее типичные генетические дефекты
- 1. 3. Белки-маркеры и ДНК-маркеры: сопоставление
- 1. 4. Циркуляция ДНК в организме
- 1. 5. Клинические объекты и цели
- 1. 6. Практические применения и специфические проблемы
- 1. 7. ДНК-маркеры и возможности скрининга
- 2. МАТЕРИАЛЫ И МЕТОДЫ
- 2. 1. Эксперименты на лабораторных животных
- 2. 2. Клинические эксперименты
- 2. 3. Молекулярные методы 34 Детекция У-специфических последовательностей 34 (стандартная и «гнездная» ПЦР)
- Детекция микросателлитных последовательностей
- Детекция мутаций К-МБ методом ЯТЬР («обогащенная»
- Детекция мутаций К-&4Я методом 88СР
- Детекция мутаций К-/Ш методом секвенирования по
- Сэнгеру
- Метод гибридизации-элонгации фрагментов ДНК
- Плавление ДНК: «закрытый» и «открытый» формат
- Пост-амплификационная оптимизация условий плавления 41 ДНК
- Иммобилизация полинуклеотидов на магнитных гранулах
- Плавление ДНК в режиме высокого разрешения (Н1ША)
- 3. РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЯ
- 3. 1. Обнаружение трансренальной ДНК
- 3. 1. 1. ДНК преодолевает почечный барьер грызунов
- 3. 1. 2. ДНК преодолевает почечный барьер человека
- 3. 2. Трансренальная ДНК как объект генодиагностики
- 3. 2. 1. Анализ микросателлитных последовательностей
- 3. 2. 2. Выявление мутаций К -МБ в трансренальной ДНК
- 3. 3. Разработка новых методических приемов
- 3. 3. 1. Шаблон-опосредованная ПЦР
- 3. 3. 2. Метод гибридизации-элонгации фрагментов ДНК 60 3.3.3 Сканирование мутаций методом плавления ДНК: применение «открытого формата»
- 3. 1. Обнаружение трансренальной ДНК
- 3. 3. 4. Мутации К-ЯАЯ в ДНК клинических образцов: 80 сопоставление методов обнаружения
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