Разработка иммуноферментной тест-системы для выявления антител к цирковирусу свиней второго типа
Диссертация
Распространение ЦВС-2 в свиноводческих хозяйствах различных стран мира оценивают по обнаружению вирус-специфических антител в сыворотке крови свиней методами иммуноферментного анализа (ИФА), непрямой имму-нофлюоресценции (РИФ) и выявлению вируса, вирусного антигена или вирусной ДНК в тканях и органах поросят методами электронной микроскопии, гибридизации in situ, полимеразной цепной реакции… Читать ещё >
Содержание
- 1. ВВЕДЕНИЕ
- 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Биологические особенности цирковируса свиней второго типа
- 2. 2. Методы выделения и культивирования ЦВС
- 2. 3. Патологии, обусловленные ЦВСцирковирусные болезни)
- 2. 4. Диагностика, профилактика и меры борьбы с ЦВС
- СОБСТВЕННЫЕ ИССЛЕДОВАНИЯ
- 3. МАТЕРИАЛЫ И МЕТОДЫ
- 4. РЕЗУЛЬТАТЫ ИССЛЕДОВАНИЙ
- 4. 1. Получение и характеристика рекомбинантного антигена ORF2 ЦВС
- 4. 1. 1. Создание генноинженерных конструкций для экспрессии генов нуклеокапсидного белка ORF
- 4. 1. Получение и характеристика рекомбинантного антигена ORF2 ЦВС
- 4. 1. 2. Очистка и иммунохимическая характеристика рекомбинатного нуклеокапсидного ORF2 белка
- 4. 2. Выбор и характеристика конъюгата
- 4. 3. Разработка непрямого твердофазного ИФА для выявления антител к цирковирусу свиней второго типа
- 4. 3. 1. Отработка условий постановки ИФА
- 4. 3. 2. Оптимизация условий проведения ИФА при исследовании проб в одном разведении
- 4. 3. 3. Оценка диагностических параметров разработанной иммуноферментной тест-системы
- 4. 4. Разработка технологии изготовления ИФА-набора для выявления антител к цирковирусу свиней второго типа («ЦИРКО-СЕРОТЕСТ»)
- 4. 4. 1. Получение и характеристика положительного и отрицательного контролей для ИФА-набора
- 4. 4. 2. Определение стабильности специфических компонентов ИФА-набора при хранении
- 4. 4. 3. Оптимизация комплектации ИФА-набора и разработка инструкции по его применению
- 4. 4. 4. Контроль воспроизводимости результатов, полученных с помощью ИФА-набора «ЦИРКО-СЕРОТЕСТ»
- 4. 5. Применение ИФА-набора «ЦИРКО-СЕРОТЕСТ» для эпизоотологического мониторинга цирковирусной инфекции в свиноводческих хозяйствах
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- Патент Fr. 9 808 777. Int C17: A 61 К 39/23, A 61 P 31/12, 37/04. Vaccin circovirus et parvovirus porcine / Allan G., Meehan В., Ellis J., et al. Date de mise a la disposition du public de la demande: 21.01.00. Bulletin 00/03.