Влияние цитозинового металирования ДНК на биосинтез резвератрола в клеточной культуре винограда амурского Vitis amurensis Rupr
Диссертация
Результаты работы были представлены на конференции «Механизм и биология сайленсинга» (США, 2011) — на X региональной конференции студентов, аспирантов вузов и научных организаций Дальнего Востока России (Владивосток, 2011) — на конференции «Эпигеномика» (США, 2012) — на XI региональной конференции студентов, аспирантов вузов и научных организаций Дальнего Востока России (Владивосток, 2012… Читать ещё >
Содержание
- список сокращений. введение
- глава 1. обзор литературы
- 1. 1. Резвератрол и другие стильбены растений
- 1. 2. Биосинтез резвератрола в клетках растений
- 1. 3. Биологически-активные свойства резвератрола
- 1. 4. Регуляция биосинтеза резвератрола
- 1. 5. Биотехнологические методы получения резвератрола
- 1. 6. Цитозиновое метилирование ДНК
- 1. 7. Биологическая значимость цитозинового метилирования ДНК и гистонов у растений
- 1. 8. Цитозиновые метилтрансферазы растений
- 1. 9. Деметилирование ДНК в клетках растений
- глава 2. материалы и методы
- 2. 1. Растительный материал и культура клеток V. атигет
- 2. 2. Питательная среда для пассирования клеточной культуры
- 2. 3. Выделение ДНК
- 2. 4. Обработка метил чувствительной рестриктазой
- 2. 5. Реакция бисульфитной конверсии
- 2. 6. Выделение РНК и получение кДНК
- 2. 7. Анализ общей экспрессии генов УаМе1, УаСМТ, УаОЯМ, ¥-аОет с помощью вырожденных праймеров
- 2. 8. Секвенирование ДНК и анализ нуклеотидных и аминокислотных последовательностей
- 2. 9. Количественный анализ экспрессии генов УаМе1, УаСМГ, УаОЯМ, УсгБет, го1 В, УаБТБ с помощью ПЦР РВ
- 2. 9. Определение содержания стильбенов в культурах клеток V. атигет’гх
- 2. 10. Статистическая обработка полученных результатов
- глава 3. результаты
- 3. 1. Стабильность биосинтеза резвератрола в трансгенных клеточных линиях винограда V. атигет
- 3. 2. Влияние деметилирования ДНК, вызванного с помощью 5-азацитидина (5А), на биосинтез резвератрола и экспрессию генов стильбен синтаз (8Т8) в культуре клеток винограда V. атигепзгя
- 3. 3. Экспрессия метилтрансфераз в культуре клеток винограда V. атигет1 $
- 3. 4. Влияние 5А и СК на метилирование генов УоБТЯИ, УаБТ82 и УаБТБЮ
- глава 4. обсуждение
- выводы
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