Аттенуация вируса энцефаломиелита мышей Тейлера путем модификации его 5-нетранслируемой области
Диссертация
Роль олигопиримидин/AUG тандема, как цис-действующего элемента, впервые была подробно исследована Пилипенко с соавторами. Было показано, что мутационные изменения (делеции, нуклеотидные замены) в области олигопиримидинового блока, вставки различной протяженности в спейсер между частями тандема существенно снижают жизнеспособность мутантных полиовирусов и матричную активность соответствующих мРНК… Читать ещё >
Содержание
- СПИСОК СОКРАЩЕНИЙ
- 1. ВВЕДЕНИЕ
- 2. ОБЗОР ЛИТЕРАТУРЫ
- 2. 1. Механизм инициации трансляции эукариотических мРНК
- 2. 2. Особенности первичной структуры генома пикорнавирусов
- 2. 3. Элементы 5-НТО пикорнавирусов, обеспечивающие внутреннее присоединение рибосом к матрице
- 2. 4. Белковый аппарат внутренней инициации трансляции
- 2. 5. Вирус энцефаломиелита мышей Тейлера. Фенотипические и структурные особенности
- 2. 6. Молекулярные основы различной биологической активности ВЭМТ
- 3. МАТЕРИАЛЫ И МЕТОДЫ
- 3. 1. Ферменты и реактивы
- 3. 2. Плазмиды
- 3. 3. Бактериальные штаммы
- 3. 4. Синтетические олигонуклеотиды
- 3. 5. Базовые методы молекулярного клонирования
- 3. 6. Определение нуклеотидной последовательности ДНК с использованием Т7 ДНК полимеразы
- 3. 7. Конструирование мутантных кДНК ВЭМТ
- 3. 8. Получение полноразмерных транскриптов
- 3. 9. Трансфекция и получение вирусных стоков
- 3. 10. Определение бляшкообразующего фенотипа
- 3. 11. Определение кинетики размножения мутантных вирусов в одноцикловом опыте
- 3. 12. Определение матричной активности мутантных РНК
- 3. 13. Исследование нейровирулентности
- 4. РЕЗУЛЬТАТЫ
- 4. 1. Геномная структура и бляшкообразующий фенотип мутантных вирусов энцефаломиелита мышей Тейлера
- 4. 2. Исследование нейровирулентности мутантных ВЭМТ с геномными перестройками в области О AT
- 4. 3. Геномная структура мутантов ВЭМТ с неинициаторным AUG в стартовом окне и их свойства in vitro
- 4. 4. Исследование нейровирулентности мутантов ВЭМТ с неинициаторным AUG в стартовом окне
- 4. 5. Структура генома вирусов, выделенных из ЦНС животных, зараженных мутантами ВЭМТ
- 5. ОБСУЖДЕНИЕ РЕЗУЛЬТАТОВ
- 5. 1. Олигопиримидин/AUG тандем является цис-активным элементом, зависимым от хозяина
- 5. 2. Функционально-структурные требования для ОАТ
- 5. 3. Точечные мутации в трансляционном стартовом окне аттенуируют вирус энцефаломиелита мышей Тейлера
- ВЫВОДЫ
- БЛАГОДАРНОСТИ
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